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@article{1811684,
author = {Śliwińska, Elwira and Loureiro, Joao and Leitch, Ilia J. and Šmarda, Petr and Bainard, Jillian and Bureš, Petr and Chumová, Zuzana and Horová, Lucie and Koutecký, Petr and Lučanová, Magdaléna and Travníček, Pavel and Galbraith, David W.},
article_location = {Hoboken},
article_number = {9},
doi = {http://dx.doi.org/10.1002/cyto.a.24499},
keywords = {cell cycle; DNA base composition; DNA content; endoreduplication; flow cytometric seed screening; genome size; in vitro cultures; intraspecific variation; ploidy},
language = {eng},
issn = {1552-4922},
journal = {Cytometry: Part A},
title = {Application-based guidelines for best practices in plant flow cytometry},
url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.a.24499},
volume = {101},
year = {2022}
}
TY - JOUR
ID - 1811684
AU - Śliwińska, Elwira - Loureiro, Joao - Leitch, Ilia J. - Šmarda, Petr - Bainard, Jillian - Bureš, Petr - Chumová, Zuzana - Horová, Lucie - Koutecký, Petr - Lučanová, Magdaléna - Travníček, Pavel - Galbraith, David W.
PY - 2022
TI - Application-based guidelines for best practices in plant flow cytometry
JF - Cytometry: Part A
VL - 101
IS - 9
SP - 749-781
EP - 749-781
PB - Wiley
SN - 15524922
KW - cell cycle
KW - DNA base composition
KW - DNA content
KW - endoreduplication
KW - flow cytometric seed screening
KW - genome size
KW - in vitro cultures
KW - intraspecific variation
KW - ploidy
UR - https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.a.24499
N2 - Flow cytometry (FCM) is currently the most widely-used method to establish nuclearDNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are suffi-cient for most plant applications, are commercially available at a reasonable price, thenumber of laboratories equipped with these instruments, and consequently newFCM users, has greatly increased over the last decade. This paper meets an urgentneed for comprehensive recommendations for best practices in FCM for differentplant science applications. We discuss advantages and limitations of establishing plantploidy, genome size, DNA base composition, cell cycle activity, and level of endo-reduplication. Applications of such measurements in plant systematics, ecology,molecular biology research, reproduction biology, tissue cultures, plant breeding, andseed sciences are described. Advice is included on how to obtain accurate and reliableresults, as well as how to manage troubleshooting that may occur during samplepreparation, cytometric measurements, and data handling. Each section is followedby best practice recommendations; tips as to what specific information should beprovided in FCM papers are also provide
ER -
$\backslash$'SLIWI$\backslash$'NSKA, Elwira, Joao LOUREIRO, Ilia J. LEITCH, Petr ŠMARDA, Jillian BAINARD, Petr BUREŠ, Zuzana CHUMOVÁ, Lucie HOROVÁ, Petr KOUTECKÝ, Magdaléna LUČANOVÁ, Pavel TRAVNÍČEK a David W. GALBRAITH. Application-based guidelines for best practices in plant flow cytometry. \textit{Cytometry: Part A}. Hoboken: Wiley, roč.~101, č.~9, s.~749-781. ISSN~1552-4922. doi:10.1002/cyto.a.24499. 2022.
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