Application-based guidelines for best practices in plant flow
cytometry

J 2022

Application-based guidelines for best practices in plant flow cytometry

ŚLIWIŃSKA, Elwira; Joao LOUREIRO; Ilia J. LEITCH; Petr ŠMARDA; Jillian BAINARD et al.

Základní údaje

Originální název

Application-based guidelines for best practices in plant flow cytometry

Autoři

Vydání

Cytometry: Part A, Hoboken, Wiley, 2022, 1552-4922

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00125097

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

cell cycle; DNA base composition; DNA content; endoreduplication; flow cytometric seed screening; genome size; in vitro cultures; intraspecific variation; ploidy

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 1. 2023 12:17, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Flow cytometry (FCM) is currently the most widely-used method to establish nuclearDNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are suffi-cient for most plant applications, are commercially available at a reasonable price, thenumber of laboratories equipped with these instruments, and consequently newFCM users, has greatly increased over the last decade. This paper meets an urgentneed for comprehensive recommendations for best practices in FCM for differentplant science applications. We discuss advantages and limitations of establishing plantploidy, genome size, DNA base composition, cell cycle activity, and level of endo-reduplication. Applications of such measurements in plant systematics, ecology,molecular biology research, reproduction biology, tissue cultures, plant breeding, andseed sciences are described. Advice is included on how to obtain accurate and reliableresults, as well as how to manage troubleshooting that may occur during samplepreparation, cytometric measurements, and data handling. Each section is followedby best practice recommendations; tips as to what specific information should beprovided in FCM papers are also provide

Návaznosti

GA19-13231S, projekt VaV

Název: Růstové strategie trvalek: od buněk k celým rostlinám

Investor: Grantová agentura ČR, Růstové strategie trvalek: od buněk k celým rostlinám

GA19-18545S, projekt VaV

Název: Eko-geografická limitace rostlinných polyploidů: experimentální testování nových hypotéz souvisejících s velikostí buněk

Investor: Grantová agentura ČR, Eko-geografická limitace rostlinných polyploidů: experimentální testování nových hypotéz souvisejících s velikostí buněk

GA20-15989S, projekt VaV

Název: Evoluce velikosti genomu - centromerický drajv v nové roli (Akronym: Centrogenomtah)

Investor: Grantová agentura ČR, Evolution of genome size - a new role for the centromere drive

Citovat

ŚLIWIŃSKA, Elwira; Joao LOUREIRO; Ilia J. LEITCH; Petr ŠMARDA; Jillian BAINARD; Petr BUREŠ; Zuzana CHUMOVÁ; Lucie HOROVÁ; Petr KOUTECKÝ; Magdaléna LUČANOVÁ; Pavel TRAVNÍČEK a David W. GALBRAITH. Application-based guidelines for best practices in plant flow cytometry. Cytometry: Part A. Hoboken: Wiley, 2022, roč. 101, č. 9, s. 749-781. ISSN 1552-4922. Dostupné z: https://doi.org/10.1002/cyto.a.24499.

@article{1811684,
   author = {Śliwińska, Elwira and Loureiro, Joao and Leitch, Ilia J. and Šmarda, Petr and Bainard, Jillian and Bureš, Petr and Chumová, Zuzana and Horová, Lucie and Koutecký, Petr and Lučanová, Magdaléna and Travníček, Pavel and Galbraith, David W.},
   article_location = {Hoboken},
   article_number = {9},
   doi = {https://doi.org/10.1002/cyto.a.24499},
   keywords = {cell cycle; DNA base composition; DNA content; endoreduplication; flow cytometric seed screening; genome size; in vitro cultures; intraspecific variation; ploidy},
   language = {eng},
   issn = {1552-4922},
   journal = {Cytometry: Part A},
   title = {Application-based guidelines for best practices in plant flow cytometry},
   url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.a.24499},
   volume = {101},
   year = {2022}
}

TY  - JOUR
ID  - 1811684
AU  - Śliwińska, Elwira - Loureiro, Joao - Leitch, Ilia J. - Šmarda, Petr - Bainard, Jillian - Bureš, Petr - Chumová, Zuzana - Horová, Lucie - Koutecký, Petr - Lučanová, Magdaléna - Travníček, Pavel - Galbraith, David W.
PY  - 2022
TI  - Application-based guidelines for best practices in plant flow cytometry
JF  - Cytometry: Part A
VL  - 101
IS  - 9
SP  - 749-781
EP  - 749-781
PB  - Wiley
SN  - 15524922
KW  - cell cycle
KW  - DNA base composition
KW  - DNA content
KW  - endoreduplication
KW  - flow cytometric seed screening
KW  - genome size
KW  - in vitro cultures
KW  - intraspecific variation
KW  - ploidy
UR  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.a.24499
N2  - Flow cytometry (FCM) is currently the most widely-used method to establish nuclearDNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are suffi-cient for most plant applications, are commercially available at a reasonable price, thenumber of laboratories equipped with these instruments, and consequently newFCM users, has greatly increased over the last decade. This paper meets an urgentneed for comprehensive recommendations for best practices in FCM for differentplant science applications. We discuss advantages and limitations of establishing plantploidy, genome size, DNA base composition, cell cycle activity, and level of endo-reduplication. Applications of such measurements in plant systematics, ecology,molecular biology research, reproduction biology, tissue cultures, plant breeding, andseed sciences are described. Advice is included on how to obtain accurate and reliableresults, as well as how to manage troubleshooting that may occur during samplepreparation, cytometric measurements, and data handling. Each section is followedby best practice recommendations; tips as to what specific information should beprovided in FCM papers are also provide
ER  -

$\backslash$'SLIWI$\backslash$'NSKA, Elwira; Joao LOUREIRO; Ilia J. LEITCH; Petr ŠMARDA; Jillian BAINARD; Petr BUREŠ; Zuzana CHUMOVÁ; Lucie HOROVÁ; Petr KOUTECKÝ; Magdaléna LUČANOVÁ; Pavel TRAVNÍČEK a David W. GALBRAITH. Application-based guidelines for best practices in plant flow cytometry. \textit{Cytometry: Part A}. Hoboken: Wiley, 2022, roč.~101, č.~9, s.~749-781. ISSN~1552-4922. Dostupné z: https://doi.org/10.1002/cyto.a.24499.

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