Expression of the apoptosis regulatory gene family in the
long-term in vitro cultured human cumulus cells

J 2021

Expression of the apoptosis regulatory gene family in the long-term in vitro cultured human cumulus cells

SIBIAK, Rafał; Rut BRYL; Katarzyna STEFAŃSKA; Błażej CHERMUŁA; Wojciech PIEŃKOWSKI et al.

Základní údaje

Originální název

Expression of the apoptosis regulatory gene family in the long-term in vitro cultured human cumulus cells

Autoři

SIBIAK, Rafał; Rut BRYL; Katarzyna STEFAŃSKA; Błażej CHERMUŁA; Wojciech PIEŃKOWSKI; Michal JEŠETA; Leszek PAWELCZYK; Paul MOZDZIAK; Robert Z SPACZYŃSKI a Bartosz KEMPISTY

Vydání

Medical Journal of Cell Biology, Warsaw, Walter de Gruyter, 2021, 2544-3577

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/21:00123324

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.2478/acb-2021-0002

EID Scopus

2-s2.0-85104466318

Klíčová slova anglicky

apoptosis; BAX; CASP9; cumulus cells; oocyte; TP53

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 12. 2021 09:14, Mgr. Tereza Miškechová

Anotace

V originále

Human cumulus cells (CCs) play a key role in the regulation of ovarian follicle maturation and oocyte fertilization. They influence the oocyte development by transferring the various molecules via the specific gap junction proteins, also known as the connexins, which provide a direct transmembrane connection between the oocyte and CCs. The human CCs were obtained in the patients diagnosed with infertility, who underwent the procedure of the controlled ovarian stimulation, and the following in vitro fertilization to elucidate the possible involvement of the CCs in the regulation of the fertilization and oocyte aging. Collected samples were long-term cultured and harvested after 7, 15, and 30 days of cultivation. Afterward, we assessed the relative expression of the following apoptosis regulatory genes - BAX, CASP9, and TP53 - using the RT-qPCR method. We noted a decrease in the expression of all above-mentioned genes in the samples harvested after 15 and 30 days, in reference to 7 days in vitro cultured CCs. In summary, our results provide precious insight into the dynamics of changes and confirm the continuous expression of the proapoptotic genes – BAX, CASP9, and TP53 in the long-term cultured CCs.

Citovat

SIBIAK, Rafał; Rut BRYL; Katarzyna STEFAŃSKA; Błażej CHERMUŁA; Wojciech PIEŃKOWSKI; Michal JEŠETA; Leszek PAWELCZYK; Paul MOZDZIAK; Robert Z SPACZYŃSKI a Bartosz KEMPISTY. Expression of the apoptosis regulatory gene family in the long-term in vitro cultured human cumulus cells. Medical Journal of Cell Biology. Warsaw: Walter de Gruyter, 2021, roč. 9, č. 1, s. 8-13. ISSN 2544-3577. Dostupné z: https://doi.org/10.2478/acb-2021-0002.

@article{1812458,
   author = {Sibiak, Rafał and Bryl, Rut and Stefańska, Katarzyna and Chermuła, Błażej and Pieńkowski, Wojciech and Ješeta, Michal and Pawelczyk, Leszek and Mozdziak, Paul and Spaczyński, Robert Z and Kempisty, Bartosz},
   article_location = {Warsaw},
   article_number = {1},
   doi = {https://doi.org/10.2478/acb-2021-0002},
   keywords = {apoptosis; BAX; CASP9; cumulus cells; oocyte; TP53},
   language = {eng},
   issn = {2544-3577},
   journal = {Medical Journal of Cell Biology},
   title = {Expression of the apoptosis regulatory gene family in the long-term in vitro cultured human cumulus cells},
   url = {https://www.sciendo.com/article/10.2478/acb-2021-0002},
   volume = {9},
   year = {2021}
}

TY  - JOUR
ID  - 1812458
AU  - Sibiak, Rafał - Bryl, Rut - Stefańska, Katarzyna - Chermuła, Błażej - Pieńkowski, Wojciech - Ješeta, Michal - Pawelczyk, Leszek - Mozdziak, Paul - Spaczyński, Robert Z - Kempisty, Bartosz
PY  - 2021
TI  - Expression of the apoptosis regulatory gene family in the long-term in vitro cultured human cumulus cells
JF  - Medical Journal of Cell Biology
VL  - 9
IS  - 1
SP  - 8-13
EP  - 8-13
PB  - Walter de Gruyter
SN  - 25443577
KW  - apoptosis
KW  - BAX
KW  - CASP9
KW  - cumulus cells
KW  - oocyte
KW  - TP53
UR  - https://www.sciendo.com/article/10.2478/acb-2021-0002
N2  - Human cumulus cells (CCs) play a key role in the regulation of ovarian follicle maturation and oocyte fertilization. They influence the oocyte development by transferring the various molecules via the specific gap junction proteins, also known as the connexins, which provide a direct transmembrane connection between the oocyte and CCs. The human CCs were obtained in the patients diagnosed with infertility, who underwent the procedure of the controlled ovarian stimulation, and the following in vitro fertilization to elucidate the possible involvement of the CCs in the regulation of the fertilization and oocyte aging. Collected samples were long-term cultured and harvested after 7, 15, and 30 days of cultivation. Afterward, we assessed the relative expression of the following apoptosis regulatory genes - BAX, CASP9, and TP53 - using the RT-qPCR method. We noted a decrease in the expression of all above-mentioned genes in the samples harvested after 15 and 30 days, in reference to 7 days in vitro cultured CCs. In summary, our results provide precious insight into the dynamics of changes and confirm the continuous expression of the proapoptotic genes – BAX, CASP9, and TP53 in the long-term cultured CCs.
ER  -

SIBIAK, Rafał; Rut BRYL; Katarzyna STEFA$\backslash$'NSKA; Bła$\backslash$.zej CHERMUŁA; Wojciech PIE$\backslash$'NKOWSKI; Michal JEŠETA; Leszek PAWELCZYK; Paul MOZDZIAK; Robert Z SPACZY$\backslash$'NSKI a Bartosz KEMPISTY. Expression of the apoptosis regulatory gene family in the long-term in vitro cultured human cumulus cells. \textit{Medical Journal of Cell Biology}. Warsaw: Walter de Gruyter, 2021, roč.~9, č.~1, s.~8-13. ISSN~2544-3577. Dostupné z: https://doi.org/10.2478/acb-2021-0002.

Přehled o publikaci