Species-specific PCR assay for the detection of Babesia
odocoilei

J 2021

Species-specific PCR assay for the detection of Babesia odocoilei

BURGESS, Hilary J.; Betty P. LOCKERBIE; Lisanework E. AYALEW; Antonia DIBERNARDO; Kristýna HRAZDILOVÁ et al.

Základní údaje

Originální název

Species-specific PCR assay for the detection of Babesia odocoilei

Autoři

BURGESS, Hilary J.; Betty P. LOCKERBIE; Lisanework E. AYALEW; Antonia DIBERNARDO; Kristýna HRAZDILOVÁ; David MODRÝ a Trent K. BOLLINGER

Vydání

Journal of Veterinary Diagnostic Investigation, SAGE Publications Inc. 2021, 1040-6387

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40300 4.3 Veterinary science

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.569

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00123524

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Babesia odocoilei; cervids; PCR; specificity

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 1. 2022 11:55, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

BURGESS, Hilary J.; Betty P. LOCKERBIE; Lisanework E. AYALEW; Antonia DIBERNARDO; Kristýna HRAZDILOVÁ; David MODRÝ a Trent K. BOLLINGER. Species-specific PCR assay for the detection of Babesia odocoilei. Journal of Veterinary Diagnostic Investigation. SAGE Publications Inc., 2021, roč. 33, č. 6, s. 1188-1192. ISSN 1040-6387. Dostupné z: https://doi.org/10.1177/10406387211032927.

@article{1817118,
   author = {Burgess, Hilary J. and Lockerbie, Betty P. and Ayalew, Lisanework E. and Dibernardo, Antonia and Hrazdilová, Kristýna and Modrý, David and Bollinger, Trent K.},
   article_number = {6},
   doi = {https://doi.org/10.1177/10406387211032927},
   keywords = {Babesia odocoilei; cervids; PCR; specificity},
   language = {eng},
   issn = {1040-6387},
   journal = {Journal of Veterinary Diagnostic Investigation},
   title = {Species-specific PCR assay for the detection of Babesia odocoilei},
   url = {https://doi.org/10.1186/s13071-017-2064-1},
   volume = {33},
   year = {2021}
}

TY  - JOUR
ID  - 1817118
AU  - Burgess, Hilary J. - Lockerbie, Betty P. - Ayalew, Lisanework E. - Dibernardo, Antonia - Hrazdilová, Kristýna - Modrý, David - Bollinger, Trent K.
PY  - 2021
TI  - Species-specific PCR assay for the detection of Babesia odocoilei
JF  - Journal of Veterinary Diagnostic Investigation
VL  - 33
IS  - 6
SP  - 1188-1192
EP  - 1188-1192
PB  - SAGE Publications Inc.
SN  - 10406387
KW  - Babesia odocoilei
KW  - cervids
KW  - PCR
KW  - specificity
UR  - https://doi.org/10.1186/s13071-017-2064-1
N2  - We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.
ER  -

BURGESS, Hilary J.; Betty P. LOCKERBIE; Lisanework E. AYALEW; Antonia DIBERNARDO; Kristýna HRAZDILOVÁ; David MODRÝ a Trent K. BOLLINGER. Species-specific PCR assay for the detection of Babesia odocoilei. \textit{Journal of Veterinary Diagnostic Investigation}. SAGE Publications Inc., 2021, roč.~33, č.~6, s.~1188-1192. ISSN~1040-6387. Dostupné z: https://doi.org/10.1177/10406387211032927.

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