Detection of Anaplasma phagocytophilum in European brown hares
(Lepus europaeus) using three different methods

J 2021

Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods

LESICZKA, Paulina Maria; David MODRÝ; Hein SPRONG; Manoj FONVILLE; Jiri PIKULA et al.

Základní údaje

Originální název

Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods

Autoři

LESICZKA, Paulina Maria; David MODRÝ; Hein SPRONG; Manoj FONVILLE; Jiri PIKULA; Vladimir PIACEK; Tomas HEGER a Kristyna HRAZDILOVA

Vydání

Zoonoses and Public Health, Hoboken, Wiley, 2021, 1863-1959

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40300 4.3 Veterinary science

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.954

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00123529

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Anaplasma phagocytophilum; digital droplet PCR; European brown hare; Lepus europaeus; qPCR; zoonosis

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2022 09:23, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

European brown hare (Lepus europaeus Pallas 1778) is a broadly distributed lagomorph species in Europe, recognized as a host for Ixodes ricinus and reservoir of a wide range of pathogens with zoonotic potential. Even though Lepus europaeus represents an important game animal in Central Europe, the data available on Anaplasma phagocytophilum in this lagomorph are scarce. In this study, three populations of brown hare from distinct localities in the Czech Republic were analysed for the presence of Anaplasma phagocytophilum DNA. We used standard qPCR, targeting the msp2 gene and adapted the same assay also for digital droplet PCR. Out of 91 samples, these two methods identified 9 and 12 as positive, respectively. For taxonomic analysis, we amplified the groEL gene from five of six samples that were found positive by both methods. In phylogenetic analyses, this haplotype belongs to ecotype 1, and to the subclade with isolates from cervids and I. ricinus. Our findings underline the importance of correct result interpretation and positivity cut-off set-up for different detection methods of A. phagocytophilum. This bacterium is characterized by a high intraspecific variability and highly sensitive detection itself, is not enough. Detailed molecular typing is necessary to define the zoonotic potential of different strains and their natural reservoirs.

Citovat

LESICZKA, Paulina Maria; David MODRÝ; Hein SPRONG; Manoj FONVILLE; Jiri PIKULA; Vladimir PIACEK; Tomas HEGER a Kristyna HRAZDILOVA. Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods. Zoonoses and Public Health. Hoboken: Wiley, 2021, roč. 68, č. 8, s. 917-925. ISSN 1863-1959. Dostupné z: https://doi.org/10.1111/zph.12883.

@article{1817157,
   author = {Lesiczka, Paulina Maria and Modrý, David and Sprong, Hein and Fonville, Manoj and Pikula, Jiri and Piacek, Vladimir and Heger, Tomas and Hrazdilova, Kristyna},
   article_location = {Hoboken},
   article_number = {8},
   doi = {https://doi.org/10.1111/zph.12883},
   keywords = {Anaplasma phagocytophilum; digital droplet PCR; European brown hare; Lepus europaeus; qPCR; zoonosis},
   language = {eng},
   issn = {1863-1959},
   journal = {Zoonoses and Public Health},
   title = {Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods},
   url = {https://doi.org/10.1111/zph.12883},
   volume = {68},
   year = {2021}
}

TY  - JOUR
ID  - 1817157
AU  - Lesiczka, Paulina Maria - Modrý, David - Sprong, Hein - Fonville, Manoj - Pikula, Jiri - Piacek, Vladimir - Heger, Tomas - Hrazdilova, Kristyna
PY  - 2021
TI  - Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods
JF  - Zoonoses and Public Health
VL  - 68
IS  - 8
SP  - 917-925
EP  - 917-925
PB  - Wiley
SN  - 18631959
KW  - Anaplasma phagocytophilum
KW  - digital droplet PCR
KW  - European brown hare
KW  - Lepus europaeus
KW  - qPCR
KW  - zoonosis
UR  - https://doi.org/10.1111/zph.12883
N2  - European brown hare (Lepus europaeus Pallas 1778) is a broadly distributed lagomorph species in Europe, recognized as a host for Ixodes ricinus and reservoir of a wide range of pathogens with zoonotic potential. Even though Lepus europaeus represents an important game animal in Central Europe, the data available on Anaplasma phagocytophilum in this lagomorph are scarce. In this study, three populations of brown hare from distinct localities in the Czech Republic were analysed for the presence of Anaplasma phagocytophilum DNA. We used standard qPCR, targeting the msp2 gene and adapted the same assay also for digital droplet PCR. Out of 91 samples, these two methods identified 9 and 12 as positive, respectively. For taxonomic analysis, we amplified the groEL gene from five of six samples that were found positive by both methods. In phylogenetic analyses, this haplotype belongs to ecotype 1, and to the subclade with isolates from cervids and I. ricinus. Our findings underline the importance of correct result interpretation and positivity cut-off set-up for different detection methods of A. phagocytophilum. This bacterium is characterized by a high intraspecific variability and highly sensitive detection itself, is not enough. Detailed molecular typing is necessary to define the zoonotic potential of different strains and their natural reservoirs.
ER  -

LESICZKA, Paulina Maria; David MODRÝ; Hein SPRONG; Manoj FONVILLE; Jiri PIKULA; Vladimir PIACEK; Tomas HEGER a Kristyna HRAZDILOVA. Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods. \textit{Zoonoses and Public Health}. Hoboken: Wiley, 2021, roč.~68, č.~8, s.~917-925. ISSN~1863-1959. Dostupné z: https://doi.org/10.1111/zph.12883.

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