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@article{1818481,
author = {Pásek, Michal and Šimurda, Jiří and Bébarová, Markéta and Christe, Georges},
article_location = {Cambridge},
article_number = {14},
doi = {http://dx.doi.org/10.1242/jcs.258228},
keywords = {Cardiac myocyte; t-tubules; Na-Ca exchanger; Membrane current density; Log-normal distribution; Monte Carlo simulation},
language = {eng},
issn = {0021-9533},
journal = {Journal of Cell Science},
title = {Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes},
url = {https://journals.biologists.com/jcs/article-abstract/134/14/jcs258228/271058/Divergent-estimates-of-the-ratio-between-Na-Ca2?redirectedFrom=fulltext},
volume = {134},
year = {2021}
}
TY - JOUR
ID - 1818481
AU - Pásek, Michal - Šimurda, Jiří - Bébarová, Markéta - Christe, Georges
PY - 2021
TI - Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes
JF - Journal of Cell Science
VL - 134
IS - 14
SP - 1-7
EP - 1-7
PB - Company of Biologists Ltd.
SN - 00219533
KW - Cardiac myocyte
KW - t-tubules
KW - Na-Ca exchanger
KW - Membrane current density
KW - Log-normal distribution
KW - Monte Carlo simulation
UR - https://journals.biologists.com/jcs/article-abstract/134/14/jcs258228/271058/Divergent-estimates-of-the-ratio-between-Na-Ca2?redirectedFrom=fulltext
N2 - The ratio between Na+-Ca2+ exchange current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes (J(NaCa)-ratio) estimated from electrophysiological data published to date yields strikingly different values between 1.7 and nearly 40. Possible reasons for such divergence were analysed by Monte Carlo simulations assuming both normal and log-normal distribution of the measured data. The confidence intervals CI95 of themean J(NaCa)-ratios computed from the reported data showed an overlap of values between 1 and 3, and between 0.3 and 4.3 in the case of normal and log-normal distribution, respectively. Further analyses revealed that the published high values likely result from a large scatter of data due to transmural differences in J(NaCa), dispersion of cell membrane capacitances and variability in incomplete detubulation. Taking into account the asymmetric distribution of the measured data, the reduction of mean current densities after detubulation and the substantiallysmaller CI95 of lower values of the mean J(NaCa)-ratio, the values between 1.6 and 3.2 may be considered as the most accurate estimates. This implies that 40 to 60% of Na+-Ca2+ exchanger is located at the t-tubular membrane of adult rat ventricular cardiomyocytes.
ER -
PÁSEK, Michal, Jiří ŠIMURDA, Markéta BÉBAROVÁ and Georges CHRISTE. Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., 2021, vol.~134, No~14, p.~1-7. ISSN~0021-9533. Available from: https://dx.doi.org/10.1242/jcs.258228.
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