Hierarchically Structured Polystyrene-Based Surfaces Amplifying
Fluorescence Signals: Cytocompatibility with Human Induced
Pluripotent Stem Cell

J 2021

Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell

SKOPALOVÁ, Kateřina; Katarzyna Anna RADASZKIEWICZ; Markéta KADLEČKOVÁ; Jiří PACHERNÍK; Antonín MINAŘÍK et al.

Základní údaje

Originální název

Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell

Autoři

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20501 Materials engineering

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00119562

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

biomimetic; surfaces; human-induced pluripotent stem cells; fluorescence signal; cardiomyogenesis

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2022 10:48, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm2; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm2; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm2) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca2+ flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.

Návaznosti

GA18-18235S, projekt VaV

Název: Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk (Akronym: KarPKB)

Investor: Grantová agentura ČR, Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk

GA19-16861S, projekt VaV

Název: Interakce biomateriálů s kmenovými buňkami v simulovaných in vivo podmínkách (Akronym: IBMSKB)

Investor: Grantová agentura ČR, Interakce biomateriálů s kmenovými buňkami v simulovaných in vivo podmínkách

Citovat

SKOPALOVÁ, Kateřina; Katarzyna Anna RADASZKIEWICZ; Markéta KADLEČKOVÁ; Jiří PACHERNÍK; Antonín MINAŘÍK; Zdenka CAPÁKOVÁ; Věra KAŠPÁRKOVÁ; Aleš MRÁČEK; Eliška DAĎOVÁ a Petr HUMPOLÍČEK. Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč. 22, č. 21, s. 11943-11954. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms222111943.

@article{1820730,
   author = {Skopalová, Kateřina and Radaszkiewicz, Katarzyna Anna and Kadlečková, Markéta and Pacherník, Jiří and Minařík, Antonín and Capáková, Zdenka and Kašpárková, Věra and Mráček, Aleš and Daďová, Eliška and Humpolíček, Petr},
   article_location = {Basel},
   article_number = {21},
   doi = {https://doi.org/10.3390/ijms222111943},
   keywords = {biomimetic; surfaces; human-induced pluripotent stem cells; fluorescence signal; cardiomyogenesis},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell},
   url = {https://www.mdpi.com/1422-0067/22/21/11943},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1820730
AU  - Skopalová, Kateřina - Radaszkiewicz, Katarzyna Anna - Kadlečková, Markéta - Pacherník, Jiří - Minařík, Antonín - Capáková, Zdenka - Kašpárková, Věra - Mráček, Aleš - Daďová, Eliška - Humpolíček, Petr
PY  - 2021
TI  - Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 21
SP  - 11943
EP  - 11943
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - biomimetic
KW  - surfaces
KW  - human-induced pluripotent stem cells
KW  - fluorescence signal
KW  - cardiomyogenesis
UR  - https://www.mdpi.com/1422-0067/22/21/11943
N2  - An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm2; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm2; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm2) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca2+ flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.
ER  -

SKOPALOVÁ, Kateřina; Katarzyna Anna RADASZKIEWICZ; Markéta KADLEČKOVÁ; Jiří PACHERNÍK; Antonín MINAŘÍK; Zdenka CAPÁKOVÁ; Věra KAŠPÁRKOVÁ; Aleš MRÁČEK; Eliška DAĎOVÁ a Petr HUMPOLÍČEK. Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč.~22, č.~21, s.~11943-11954. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms222111943.

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