The yeast mitochondrial succinylome: Implications for
regulation of mitochondrial nucleoids

J 2021

The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids

FRANKOVSKÝ, Ján, Barbora KERESZTESOVÁ, Jana BELLOVÁ, Nina KUNOVÁ, Nikola ČANIGOVÁ et. al.

Základní údaje

Originální název

The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids

Autoři

FRANKOVSKÝ, Ján (703 Slovensko), Barbora KERESZTESOVÁ (703 Slovensko), Jana BELLOVÁ (703 Slovensko), Nina KUNOVÁ (703 Slovensko), Nikola ČANIGOVÁ (703 Slovensko), Kateřina HANÁKOVÁ (203 Česká republika, domácí), Jacob A. BAUER (703 Slovensko), Gabriela ONDROVIČOVÁ (703 Slovensko), Veronika LUKÁČOVÁ (703 Slovensko), Barbara SIVÁKOVÁ (703 Slovensko), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí), Vladimír PEVALA (703 Slovensko), Katarína PROCHÁZKOVÁ (703 Slovensko), Jozef NOSEK (703 Slovensko), Peter BARÁTH (703 Slovensko), Eva KUTEJOVÁ (703 Slovensko) a Ľubomír TOMÁŠKA (703 Slovensko)

Vydání

The Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2021, 0021-9258

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 5.157 v roce 2020

Kód RIV

RIV/00216224:14740/21:00123803

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.jbc.2021.101155

UT WoS

000713011000011

Klíčová slova anglicky

DNA–protein interaction lysine succinylation mitochondria mitochondrial DNA mitochondrial nucleoid post-translational modification (PTM) proteomics succinylome yeast

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:41, Ing. Martina Blahová

Anotace

V originále

Acylation modifications, such as the succinylation of lysine, are post-translational modifications and a powerful means of regulating protein activity. Some acylations occur non enzymatically, driven by an increase in the concentration of acyl group donors. Lysine succinylation has a profound effect on the corresponding site within the protein, as it dramatically changes the charge of the residue. In eukaryotes, it predominantly affects mitochondrial proteins because the donor of succinate, succinyl-CoA, is primarily generated in the tricarboxylic acid cycle. Although numerous succinylated mitochondrial proteins have been identified in Saccharomyces cerevisiae, a more detailed characterization of the yeast mitochondrial succinylome is still lacking. Here, we performed a proteomic MS analysis of purified yeast mitochondria and detected 314 succinylated mitochondrial proteins with 1763 novel succinylation sites. The mitochondrial nucleoid, a complex of mitochondrial DNA and mitochondrial proteins, is one of the structures whose protein components are affected by succinylation. We found that Abf2p, the principal component of mitochondrial nucleoids responsible for compacting mitochondrial DNA in S. cerevisiae, can be succinylated in vivo on at least thirteen lysine residues. Abf2p succinylation in vitro inhibits its DNA-binding activity and reduces its sensitivity to digestion by the ATP-dependent ScLon protease. We conclude that changes in the metabolic state of a cell resulting in an increase in the concentration of tricarboxylic acid intermediates may affect mitochondrial functions.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

FRANKOVSKÝ, Ján, Barbora KERESZTESOVÁ, Jana BELLOVÁ, Nina KUNOVÁ, Nikola ČANIGOVÁ, Kateřina HANÁKOVÁ, Jacob A. BAUER, Gabriela ONDROVIČOVÁ, Veronika LUKÁČOVÁ, Barbara SIVÁKOVÁ, Zbyněk ZDRÁHAL, Vladimír PEVALA, Katarína PROCHÁZKOVÁ, Jozef NOSEK, Peter BARÁTH, Eva KUTEJOVÁ a Ľubomír TOMÁŠKA. The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids. The Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 2021, roč. 297, č. 4, s. "101155", 16 s. ISSN 0021-9258. Dostupné z: https://dx.doi.org/10.1016/j.jbc.2021.101155.

@article{1823286,
   author = {Frankovský, Ján and Keresztesová, Barbora and Bellová, Jana and Kunová, Nina and Čanigová, Nikola and Hanáková, Kateřina and Bauer, Jacob A. and Ondrovičová, Gabriela and Lukáčová, Veronika and Siváková, Barbara and Zdráhal, Zbyněk and Pevala, Vladimír and Procházková, Katarína and Nosek, Jozef and Baráth, Peter and Kutejová, Eva and Tomáška, Ľubomír},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.jbc.2021.101155},
   keywords = {DNA–protein interaction lysine succinylation mitochondria mitochondrial DNA mitochondrial nucleoid post-translational modification (PTM) proteomics succinylome yeast},
   language = {eng},
   issn = {0021-9258},
   journal = {The Journal of Biological Chemistry},
   title = {The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids},
   url = {https://www.sciencedirect.com/science/article/pii/S0021925821009571?via%3Dihub},
   volume = {297},
   year = {2021}
}

TY  - JOUR
ID  - 1823286
AU  - Frankovský, Ján - Keresztesová, Barbora - Bellová, Jana - Kunová, Nina - Čanigová, Nikola - Hanáková, Kateřina - Bauer, Jacob A. - Ondrovičová, Gabriela - Lukáčová, Veronika - Siváková, Barbara - Zdráhal, Zbyněk - Pevala, Vladimír - Procházková, Katarína - Nosek, Jozef - Baráth, Peter - Kutejová, Eva - Tomáška, Ľubomír
PY  - 2021
TI  - The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids
JF  - The Journal of Biological Chemistry
VL  - 297
IS  - 4
SP  - "101155"
EP  - "101155"
PB  - American Society for Biochemistry and Molecular Biology
SN  - 00219258
KW  - DNA–protein interaction lysine succinylation mitochondria mitochondrial DNA mitochondrial nucleoid post-translational modification (PTM) proteomics succinylome yeast
UR  - https://www.sciencedirect.com/science/article/pii/S0021925821009571?via%3Dihub
N2  - Acylation modifications, such as the succinylation of lysine, are post-translational modifications and a powerful means of regulating protein activity. Some acylations occur non enzymatically, driven by an increase in the concentration of acyl group donors. Lysine succinylation has a profound effect on the corresponding site within the protein, as it dramatically changes the charge of the residue. In eukaryotes, it predominantly affects mitochondrial proteins because the donor of succinate, succinyl-CoA, is primarily generated in the tricarboxylic acid cycle. Although numerous succinylated mitochondrial proteins have been identified in Saccharomyces cerevisiae, a more detailed characterization of the yeast mitochondrial succinylome is still lacking. Here, we performed a proteomic MS analysis of purified yeast mitochondria and detected 314 succinylated mitochondrial proteins with 1763 novel succinylation sites. The mitochondrial nucleoid, a complex of mitochondrial DNA and mitochondrial proteins, is one of the structures whose protein components are affected by succinylation. We found that Abf2p, the principal component of mitochondrial nucleoids responsible for compacting mitochondrial DNA in S. cerevisiae, can be succinylated in vivo on at least thirteen lysine residues. Abf2p succinylation in vitro inhibits its DNA-binding activity and reduces its sensitivity to digestion by the ATP-dependent ScLon protease. We conclude that changes in the metabolic state of a cell resulting in an increase in the concentration of tricarboxylic acid intermediates may affect mitochondrial functions.
ER  -

FRANKOVSKÝ, Ján, Barbora KERESZTESOVÁ, Jana BELLOVÁ, Nina KUNOVÁ, Nikola ČANIGOVÁ, Kateřina HANÁKOVÁ, Jacob A. BAUER, Gabriela ONDROVIČOVÁ, Veronika LUKÁČOVÁ, Barbara SIVÁKOVÁ, Zbyněk ZDRÁHAL, Vladimír PEVALA, Katarína PROCHÁZKOVÁ, Jozef NOSEK, Peter BARÁTH, Eva KUTEJOVÁ a Ľubomír TOMÁŠKA. The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids. \textit{The Journal of Biological Chemistry}. American Society for Biochemistry and Molecular Biology, 2021, roč.~297, č.~4, s.~''101155'', 16 s. ISSN~0021-9258. Dostupné z: https://dx.doi.org/10.1016/j.jbc.2021.101155.

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