Elegant, rapid and simple procedure for labeling of antibodies
with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS
detection

D 2021

Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

ASSI, Navid; Kristyna PAVELICOVA; Marketa VACULOVICOVA a Tomáš VACULOVIČ

Základní údaje

Originální název

Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Autoři

ASSI, Navid; Kristyna PAVELICOVA; Marketa VACULOVICOVA a Tomáš VACULOVIČ

Vydání

Ostrava, 12th International Conference on Nanomaterials - Research and Application, NANOCON 2020, od s. 395-398, 4 s. 2021

Nakladatel

TANGER Ltd.

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00123819

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-87294-98-7

ISSN

Klíčová slova anglicky

Antibody; antigen; quantum dots; cleavage disulfide bonds; fluorescent

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 1. 2022 18:10, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).

Citovat

ASSI, Navid; Kristyna PAVELICOVA; Marketa VACULOVICOVA a Tomáš VACULOVIČ. Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection. Online. In 12th International Conference on Nanomaterials - Research and Application, NANOCON 2020. Ostrava: TANGER Ltd., 2021, s. 395-398. ISBN 978-80-87294-98-7. Dostupné z: https://doi.org/10.37904/nanocon.2020.3752.

@inproceedings{1823518,
   author = {Assi, Navid and Pavelicova, Kristyna and Vaculovicova, Marketa and Vaculovič, Tomáš},
   address = {Ostrava},
   booktitle = {12th International Conference on Nanomaterials - Research and Application, NANOCON 2020},
   doi = {https://doi.org/10.37904/nanocon.2020.3752},
   keywords = {Antibody; antigen; quantum dots; cleavage disulfide bonds; fluorescent},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Ostrava},
   isbn = {978-80-87294-98-7},
   pages = {395-398},
   publisher = {TANGER Ltd.},
   title = {Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection},
   url = {https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection},
   year = {2021}
}

TY  - CONF
ID  - 1823518
AU  - Assi, Navid - Pavelicova, Kristyna - Vaculovicova, Marketa - Vaculovič, Tomáš
PY  - 2021
TI  - Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection
PB  - TANGER Ltd.
CY  - Ostrava
SN  - 9788087294987
KW  - Antibody
KW  - antigen
KW  - quantum dots
KW  - cleavage disulfide bonds
KW  - fluorescent
UR  - https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection
N2  - Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).
ER  -

ASSI, Navid; Kristyna PAVELICOVA; Marketa VACULOVICOVA a Tomáš VACULOVIČ. Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection. Online. In \textit{12th International Conference on Nanomaterials - Research and Application, NANOCON 2020}. Ostrava: TANGER Ltd., 2021, s.~395-398. ISBN~978-80-87294-98-7. Dostupné z: https://doi.org/10.37904/nanocon.2020.3752.

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