Conductive Polymer PEDOT:PSS-Based Platform for Embryonic
Stem-Cell Differentiation

J 2022

Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation

VOJTKOVÁ, Eva; Jiří EHLICH; Stanislav STŘÍTESKÝ; Martin VALA; Martin WEITER et al.

Základní údaje

Originální název

Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation

Autoři

VOJTKOVÁ, Eva; Jiří EHLICH; Stanislav STŘÍTESKÝ; Martin VALA; Martin WEITER; Jiří PACHERNÍK; Lukáš KUBALA a Jan VÍTEČEK

Vydání

International Journal of Molecular Sciences, MDPI, 2022, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00119641

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

conductive polymer; PEDOT:PSS; screen print; embryonic stem cells; electrostimulation

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 11. 2024 14:10, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.

Návaznosti

GA18-18235S, projekt VaV

Název: Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk (Akronym: KarPKB)

Investor: Grantová agentura ČR, Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk

Citovat

VOJTKOVÁ, Eva; Jiří EHLICH; Stanislav STŘÍTESKÝ; Martin VALA; Martin WEITER; Jiří PACHERNÍK; Lukáš KUBALA a Jan VÍTEČEK. Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation. International Journal of Molecular Sciences. MDPI, 2022, roč. 23, č. 3, s. 1-16. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms23031107.

@article{1832630,
   author = {Vojtková, Eva and Ehlich, Jiří and Stříteský, Stanislav and Vala, Martin and Weiter, Martin and Pacherník, Jiří and Kubala, Lukáš and Víteček, Jan},
   article_number = {3},
   doi = {https://doi.org/10.3390/ijms23031107},
   keywords = {conductive polymer; PEDOT:PSS; screen print; embryonic stem cells; electrostimulation},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation},
   url = {https://www.mdpi.com/1422-0067/23/3/1107},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 1832630
AU  - Vojtková, Eva - Ehlich, Jiří - Stříteský, Stanislav - Vala, Martin - Weiter, Martin - Pacherník, Jiří - Kubala, Lukáš - Víteček, Jan
PY  - 2022
TI  - Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 3
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 14220067
KW  - conductive polymer
KW  - PEDOT:PSS
KW  - screen print
KW  - embryonic stem cells
KW  - electrostimulation
UR  - https://www.mdpi.com/1422-0067/23/3/1107
N2  - Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.
ER  -

VOJTKOVÁ, Eva; Jiří EHLICH; Stanislav STŘÍTESKÝ; Martin VALA; Martin WEITER; Jiří PACHERNÍK; Lukáš KUBALA a Jan VÍTEČEK. Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation. \textit{International Journal of Molecular Sciences}. MDPI, 2022, roč.~23, č.~3, s.~1-16. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms23031107.

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