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@proceedings{1834178,
author = {Ilgová, Jana and Škorpíková, Lucie and Vorel, Jiří and Roudnický, Pavel and Horn, Martin and Kašný, Martin},
booktitle = {XXVI. Helmintologické dny},
keywords = {Fasciola hepatica; eggs;embryonation; peptidases; omics},
language = {eng},
isbn = {978-80-906644-9-4},
title = {Changes of peptidase profile during Fasciola hepatica embryonation},
year = {2021}
}
TY - CONF
ID - 1834178
AU - Ilgová, Jana - Škorpíková, Lucie - Vorel, Jiří - Roudnický, Pavel - Horn, Martin - Kašný, Martin
PY - 2021
TI - Changes of peptidase profile during Fasciola hepatica embryonation
SN - 9788090664494
KW - Fasciola hepatica
KW - eggs;embryonation
KW - peptidases
KW - omics
N2 - Fasciola hepatica is a widespread foodborne trematode that parasitizes a broad range of mammals, including humans. Adults of this cosmopolitan species lay their propagation stages - unsegmented operculated eggs, into the bile duct of their mammalian host. Crucial aspects of F. hepatica life cycle, such as migration, reproduction and immune evasion, are directly dependent on the activity of proteolytic enzymes, also known as peptidases. Expression of these molecules have been intensely studied in metacercariae, newly excysted juveniles and adults, however embryonic stages of F. hepatica, have not yielded much scientific attention in this context. We used combined transcriptomic/proteomic approach which led to the identification of expressed and translated peptidases in developing eggs of different age. Additionally, activity profiling with the substrate library and selective inhibitors identified presence of different peptidase classes in egg extracts. Our results indicate that F. hepatica egg transcriptome remains mostly constant, while the proteome is being considerably adjusted throughout the embryonation. Threonine catalytic subunits of proteasome are constantly produced, while the cysteine peptidases prevailing in freshly laid eggs are supplemented by aspartic peptidases and metallopeptidases in the later stages of egg development.
ER -
ILGOVÁ, Jana, Lucie ŠKORPÍKOVÁ, Jiří VOREL, Pavel ROUDNICKÝ, Martin HORN a Martin KAŠNÝ. Changes of peptidase profile during Fasciola hepatica embryonation. In \textit{XXVI. Helmintologické dny}. 2021. ISBN~978-80-906644-9-4.
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