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@article{1834920, author = {Levien, Monique and Farka, Zdeněk and Pastucha, Matěj and Skládal, Petr and Nasri, Zahra and Weltmann, KlausandDieter and Fricke, Katja}, article_number = {May}, doi = {http://dx.doi.org/10.1016/j.apsusc.2022.152511}, keywords = {Hydrogel coating; Functional surface; Atmospheric pressure plasma polymerization; Electrochemical biosensor; Amperometry; Glucose oxidase; Acetylcholinesterase}, language = {eng}, issn = {0169-4332}, journal = {Applied Surface Science}, title = {Functional plasma-polymerized hydrogel coatings for electrochemical biosensing}, url = {https://www.sciencedirect.com/science/article/abs/pii/S0169433222000964}, volume = {584}, year = {2022} }
TY - JOUR ID - 1834920 AU - Levien, Monique - Farka, Zdeněk - Pastucha, Matěj - Skládal, Petr - Nasri, Zahra - Weltmann, Klaus-Dieter - Fricke, Katja PY - 2022 TI - Functional plasma-polymerized hydrogel coatings for electrochemical biosensing JF - Applied Surface Science VL - 584 IS - May SP - 1-8 EP - 1-8 PB - Elsevier SN - 01694332 KW - Hydrogel coating KW - Functional surface KW - Atmospheric pressure plasma polymerization KW - Electrochemical biosensor KW - Amperometry KW - Glucose oxidase KW - Acetylcholinesterase UR - https://www.sciencedirect.com/science/article/abs/pii/S0169433222000964 N2 - Acrylate-based hydrogels with multifunctional properties have proven to be suitable candidates for the development of sensor systems. They gained popularity especially in combination with bioelectronics, as there is a need to understand and control the interactions of bionic devices with the human body and other environments. In this study, we present results on the biointeraction capability of plasma-polymerized (pp) hydrogels made of hydroxyethyl methacrylate (HEMA) and 2-(diethylamino)ethyl methacrylate (DEAEMA) mixtures on gold screen-printed electrodes (SPE). The hydrogels were generated by an atmospheric pressure plasma jet, and their chemical composition was characterized via FT-IR. The FT-IR analysis revealed several functional groups suitable for biomolecule immobilization, whereas the amount of -C-N, –OH, and -C-O-C groups differs depending on the mixture ratios. The pp HEMA:DEAEMA (HD) hydrogel coatings provide alternative interfacing materials for electrochemical biosensing. The enzymes glucose oxidase (GOx) and acetylcholinesterase (AChE) were coupled to the hydrogel-based surfaces, and the effects of the mixture ratios on the biomolecule immobilization were investigated. It is possible to address different functional groups of the mixtures with different immobilization strategies; thus, the sensor response can be optimized. Finally, glucose as GOx substrate and eserine as AChE inhibitor were detected by amperometry to demonstrate the practical biosensing applicability of the coatings. ER -
LEVIEN, Monique, Zdeněk FARKA, Matěj PASTUCHA, Petr SKLÁDAL, Zahra NASRI, Klaus-Dieter WELTMANN a Katja FRICKE. Functional plasma-polymerized hydrogel coatings for electrochemical biosensing. \textit{Applied Surface Science}. Elsevier, 2022, roč.~584, May, s.~1-8. ISSN~0169-4332. Dostupné z: https://dx.doi.org/10.1016/j.apsusc.2022.152511.
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