The comprehensive interactomes of human adenosine RNA
methyltransferases and demethylases reveal distinct functional
and regulatory features

J 2021

The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features

COVELO MOLARES, Helena; Aleš OBRDLÍK; I. POSTULKOVA; Michaela DOHNÁLKOVÁ; Pavlína GREGOROVÁ et al.

Základní údaje

Originální název

The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features

Autoři

COVELO MOLARES, Helena; Aleš OBRDLÍK; I. POSTULKOVA; Michaela DOHNÁLKOVÁ; Pavlína GREGOROVÁ; Sri Ranjani GANJI; David POTĚŠIL; L. GAWRIYSKI; M. VARJOSALO a Štěpánka VAŇÁČOVÁ

Vydání

Nucleic acids research, Oxford, Oxford University Press, 2021, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 19.160

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/21:00119659

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

PRE-MESSENGER-RNA; SINGLE-STRANDED-DNA; POLYMERASE-II; NUCLEAR-RNA; M(6)A METHYLTRANSFERASE; GENE-EXPRESSION; 7SK SNRNP; P-TEFB; METHYLATION; PROTEIN

Štítky

CF CELLIM, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:47, Ing. Martina Blahová

Anotace

V originále

N6-methyladenosine (m(6)A) and N6,2 '-O-dimethyladenosine (m(6)Am) are two abundant modifications found in mRNAs and ncRNAs that can regulate multiple aspects of RNA biology. They function mainly by regulating interactions with specific RNA-binding proteins. Both modifications are linked to development, disease and stress response. To date, three methyltransferases and two demethylases have been identified that modify adenosines in mammalian mRNAs. Here, we present a comprehensive analysis of the interactomes of these enzymes. PCIF1 protein network comprises mostly factors involved in nascent RNA synthesis by RNA polymerase II, whereas ALKBH5 is closely linked with most aspects of pre-mRNA processing and mRNA export to the cytoplasm. METTL16 resides in subcellular compartments co-inhabited by several other RNA modifiers and processing factors. FTO interactome positions this demethylase at a crossroad between RNA transcription, RNA processing and DNA replication and repair. Altogether, these enzymes share limited spatial interactomes, pointing to specific molecular mechanisms of their regulation.

Návaznosti

EF19_074/0012727, projekt VaV

Název: MSCAfellow3@MUNI

GA19-21829S, projekt VaV

Název: Charakterizace molekulárních mechanismů lidských adenosinových demetyláz

Investor: Grantová agentura ČR, Charakterizace molekulárních mechanismů lidských adenosinových demetyláz

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

LTC18052, projekt VaV

Název: Integrativní studium molekulárních mechanismů epitranskriptomu savčích buněk

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Integrativní studium molekulárních mechanismů epitranskriptomu savčích buněk, INTER-COST

90127, velká výzkumná infrastruktura

Název: CIISB II

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Přiložené soubory

The_comprehensive_interactomes_of_human.pdf

Citovat

COVELO MOLARES, Helena; Aleš OBRDLÍK; I. POSTULKOVA; Michaela DOHNÁLKOVÁ; Pavlína GREGOROVÁ; Sri Ranjani GANJI; David POTĚŠIL; L. GAWRIYSKI; M. VARJOSALO a Štěpánka VAŇÁČOVÁ. The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features. Nucleic acids research. Oxford: Oxford University Press, 2021, roč. 49, č. 19, s. 10895-10910. ISSN 0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkab900.

@article{1835965,
   author = {Covelo Molares, Helena and Obrdlík, Aleš and Postulkova, I. and Dohnálková, Michaela and Gregorová, Pavlína and Ganji, Sri Ranjani and Potěšil, David and Gawriyski, L. and Varjosalo, M. and Vaňáčová, Štěpánka},
   article_location = {Oxford},
   article_number = {19},
   doi = {https://doi.org/10.1093/nar/gkab900},
   keywords = {PRE-MESSENGER-RNA; SINGLE-STRANDED-DNA; POLYMERASE-II; NUCLEAR-RNA; M(6)A METHYLTRANSFERASE; GENE-EXPRESSION; 7SK SNRNP; P-TEFB; METHYLATION; PROTEIN},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic acids research},
   title = {The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features},
   url = {https://watermark.silverchair.com/gkab900.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAtowggLWBgkqhkiG9w0BBwagggLHMIICwwIBADCCArwGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMYI0atmVmxuSJtvGtAgEQgIICjTOr8naIxPbW6AIOXEA-BqaQ4jGkEwiuhTJ9NCzQmkhQdI},
   volume = {49},
   year = {2021}
}

TY  - JOUR
ID  - 1835965
AU  - Covelo Molares, Helena - Obrdlík, Aleš - Postulkova, I. - Dohnálková, Michaela - Gregorová, Pavlína - Ganji, Sri Ranjani - Potěšil, David - Gawriyski, L. - Varjosalo, M. - Vaňáčová, Štěpánka
PY  - 2021
TI  - The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features
JF  - Nucleic acids research
VL  - 49
IS  - 19
SP  - 10895-10910
EP  - 10895-10910
PB  - Oxford University Press
SN  - 03051048
KW  - PRE-MESSENGER-RNA
KW  - SINGLE-STRANDED-DNA
KW  - POLYMERASE-II
KW  - NUCLEAR-RNA
KW  - M(6)A METHYLTRANSFERASE
KW  - GENE-EXPRESSION
KW  - 7SK SNRNP
KW  - P-TEFB
KW  - METHYLATION
KW  - PROTEIN
UR  - https://watermark.silverchair.com/gkab900.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAtowggLWBgkqhkiG9w0BBwagggLHMIICwwIBADCCArwGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMYI0atmVmxuSJtvGtAgEQgIICjTOr8naIxPbW6AIOXEA-BqaQ4jGkEwiuhTJ9NCzQmkhQdI
N2  - N6-methyladenosine (m(6)A) and N6,2 '-O-dimethyladenosine (m(6)Am) are two abundant modifications found in mRNAs and ncRNAs that can regulate multiple aspects of RNA biology. They function mainly by regulating interactions with specific RNA-binding proteins. Both modifications are linked to development, disease and stress response. To date, three methyltransferases and two demethylases have been identified that modify adenosines in mammalian mRNAs. Here, we present a comprehensive analysis of the interactomes of these enzymes. PCIF1 protein network comprises mostly factors involved in nascent RNA synthesis by RNA polymerase II, whereas ALKBH5 is closely linked with most aspects of pre-mRNA processing and mRNA export to the cytoplasm. METTL16 resides in subcellular compartments co-inhabited by several other RNA modifiers and processing factors. FTO interactome positions this demethylase at a crossroad between RNA transcription, RNA processing and DNA replication and repair. Altogether, these enzymes share limited spatial interactomes, pointing to specific molecular mechanisms of their regulation.
ER  -

COVELO MOLARES, Helena; Aleš OBRDLÍK; I. POSTULKOVA; Michaela DOHNÁLKOVÁ; Pavlína GREGOROVÁ; Sri Ranjani GANJI; David POTĚŠIL; L. GAWRIYSKI; M. VARJOSALO a Štěpánka VAŇÁČOVÁ. The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2021, roč.~49, č.~19, s.~10895-10910. ISSN~0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkab900.

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