The fish pathogen Flavobacterium columnare represents four
distinct species: Flavobacterium columnare, Flavobacterium
covae sp. nov., Flavobacterium davisii sp. nov. and
Flavobacterium oreochromis sp. nov., and emended description of
Flavobacterium columnare

J 2022

The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare

LAFRENTZ, Benjamin R.; Stanislava KRÁLOVÁ; Claire R. BURBICK; Trevor L. ALEXANDER; Conner W. PHILLIPS et. al.

Základní údaje

Originální název

The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare

Autoři

LAFRENTZ, Benjamin R. (garant); Stanislava KRÁLOVÁ (703 Slovensko, domácí); Claire R. BURBICK; Trevor L. ALEXANDER; Conner W. PHILLIPS; Matt J. GRIFFIN; Geoffrey C. WALDBIESER; Julio C. GARCÍA; Fernanda DE ALEXANDRE SEBASTIÃO; Esteban SOTO; Thomas P. LOCH; Mark R. LILES a Kevin R. SNEKVIK

Vydání

Systematic and Applied Microbiology, Elsevier GmbH, 2022, 0723-2020

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.400

Kód RIV

RIV/00216224:14310/22:00125420

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.syapm.2021.126293

UT WoS

000788742800002

EID Scopus

2-s2.0-85122518462

Klíčová slova anglicky

Columnaris disease; Genetic groups; Polyphasic; Taxonomy; MALDI-TOF

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 5. 2022 15:17, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Flavobacterium columnare is the causative agent of columnaris disease in freshwater fish and four discrete genetic groups exist within the species, suggesting that the species designation requires revision. The present study determined the taxonomic status of the four genetic groups of F. columnare using polyphasic and phylogenomic approaches and included five representative isolates from each genetic group (including type strain ATCC 23463T; genetic group 1). 16S rRNA gene sequence analysis revealed genetic group 2 isolate AL-02-36T, genetic group 3 isolate 90-106T, and genetic group 4 isolate Costa Rica 04-02-TNT shared less than <98.8 % sequence identity to F. columnare ATCC 23463T. Phylogenetic analyses of 16S rRNA and gyrB genes using different methodologies demonstrated the four genetic groups formed well-supported and distinct clades within the genus Flavobacterium. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (GGDC) values between F. columnare ATCC 23463T, genetic group 2 isolate AL-02-36T, genetic group 3 isolate 90-106T, and genetic group 4 isolate Costa Rica 04-02-TNT were less than 90.84% and 42.7%, respectively. Biochemical and physiological characteristics were similar among the four genetic groups; however, quantitative differences in fatty acid profiles were detected and MALDI-TOF analyses demonstrated numerous distinguishing peaks unique to each genetic group. Chemotaxonomic, MALDI-TOF characterization and ANI/GGDC calculations afforded differentiation between the genetic groups, indicating each group is a discrete species. Herein, the names F. covae sp. nov. (AL-02-36T), F. davisii sp. nov. (90-106T), and F. oreochromis sp. nov. (Costa Rica 04-02-TNT) are proposed to represent genetic groups 2, 3, and 4, respectively.

Citovat

LAFRENTZ, Benjamin R.; Stanislava KRÁLOVÁ; Claire R. BURBICK; Trevor L. ALEXANDER; Conner W. PHILLIPS; Matt J. GRIFFIN; Geoffrey C. WALDBIESER; Julio C. GARCÍA; Fernanda DE ALEXANDRE SEBASTIÃO; Esteban SOTO; Thomas P. LOCH; Mark R. LILES a Kevin R. SNEKVIK. The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare. Systematic and Applied Microbiology. Elsevier GmbH, 2022, roč. 45, č. 2, s. 1-16. ISSN 0723-2020. Dostupné z: https://doi.org/10.1016/j.syapm.2021.126293.

@article{1835967,
   author = {LaFrentz, Benjamin R. and Králová, Stanislava and Burbick, Claire R. and Alexander, Trevor L. and Phillips, Conner W. and Griffin, Matt J. and Waldbieser, Geoffrey C. and García, Julio C. and de Alexandre Sebastião, Fernanda and Soto, Esteban and Loch, Thomas P. and Liles, Mark R. and Snekvik, Kevin R.},
   article_number = {2},
   doi = {https://doi.org/10.1016/j.syapm.2021.126293},
   keywords = {Columnaris disease; Genetic groups; Polyphasic; Taxonomy; MALDI-TOF},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare},
   url = {https://www.sciencedirect.com/science/article/pii/S0723202021001168},
   volume = {45},
   year = {2022}
}

TY  - JOUR
ID  - 1835967
AU  - LaFrentz, Benjamin R. - Králová, Stanislava - Burbick, Claire R. - Alexander, Trevor L. - Phillips, Conner W. - Griffin, Matt J. - Waldbieser, Geoffrey C. - García, Julio C. - de Alexandre Sebastião, Fernanda - Soto, Esteban - Loch, Thomas P. - Liles, Mark R. - Snekvik, Kevin R.
PY  - 2022
TI  - The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare
JF  - Systematic and Applied Microbiology
VL  - 45
IS  - 2
SP  - 1-16
EP  - 1-16
PB  - Elsevier GmbH
SN  - 07232020
KW  - Columnaris disease
KW  - Genetic groups
KW  - Polyphasic
KW  - Taxonomy
KW  - MALDI-TOF
UR  - https://www.sciencedirect.com/science/article/pii/S0723202021001168
N2  - Flavobacterium columnare is the causative agent of columnaris disease in freshwater fish and four discrete genetic groups exist within the species, suggesting that the species designation requires revision. The present study determined the taxonomic status of the four genetic groups of F. columnare using polyphasic and phylogenomic approaches and included five representative isolates from each genetic group (including type strain ATCC 23463T; genetic group 1). 16S rRNA gene sequence analysis revealed genetic group 2 isolate AL-02-36T, genetic group 3 isolate 90-106T, and genetic group 4 isolate Costa Rica 04-02-TNT shared less than <98.8 % sequence identity to F. columnare ATCC 23463T. Phylogenetic analyses of 16S rRNA and gyrB genes using different methodologies demonstrated the four genetic groups formed well-supported and distinct clades within the genus Flavobacterium. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (GGDC) values between F. columnare ATCC 23463T, genetic group 2 isolate AL-02-36T, genetic group 3 isolate 90-106T, and genetic group 4 isolate Costa Rica 04-02-TNT were less than 90.84% and 42.7%, respectively. Biochemical and physiological characteristics were similar among the four genetic groups; however, quantitative differences in fatty acid profiles were detected and MALDI-TOF analyses demonstrated numerous distinguishing peaks unique to each genetic group. Chemotaxonomic, MALDI-TOF characterization and ANI/GGDC calculations afforded differentiation between the genetic groups, indicating each group is a discrete species. Herein, the names F. covae sp. nov. (AL-02-36T), F. davisii sp. nov. (90-106T), and F. oreochromis sp. nov. (Costa Rica 04-02-TNT) are proposed to represent genetic groups 2, 3, and 4, respectively.
ER  -

LAFRENTZ, Benjamin R.; Stanislava KRÁLOVÁ; Claire R. BURBICK; Trevor L. ALEXANDER; Conner W. PHILLIPS; Matt J. GRIFFIN; Geoffrey C. WALDBIESER; Julio C. GARCÍA; Fernanda DE ALEXANDRE SEBASTIÃO; Esteban SOTO; Thomas P. LOCH; Mark R. LILES a Kevin R. SNEKVIK. The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare. \textit{Systematic and Applied Microbiology}. Elsevier GmbH, 2022, roč.~45, č.~2, s.~1-16. ISSN~0723-2020. Dostupné z: https://doi.org/10.1016/j.syapm.2021.126293.

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