Insights into the Resistome and Phylogenomics of a ST195
Multidrug-Resistant Acinetobacter baumannii Clinical Isolate
from the Czech Republic

J 2021

Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic

MLYNARČÍK, Patrik; Monika DOLEJSKÁ; Iva VAGNEROVA; Jana PETRZELOVA; Iva SUKKAR et al.

Základní údaje

Originální název

Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic

Autoři

MLYNARČÍK, Patrik; Monika DOLEJSKÁ; Iva VAGNEROVA; Jana PETRZELOVA; Iva SUKKAR; Veronika ZDARSKA a Milan KOLAR

Vydání

Life, 2021, 2075-1729

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

text článku

Impakt faktor

Impact factor: 3.253

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.3390/life11101079

UT WoS

34685451

Klíčová slova anglicky

beta-lactamse, antiibotic resistance, PCR, Acinetobacter baumannii, bacteria

Změněno: 25. 2. 2022 10:09, Mgr. Tereza Miškechová

Anotace

V originále

Increasing antimicrobial resistance in nosocomial pathogens, such as Acinetobacter bauman- nii, is becoming a serious threat to public health. It is necessary to detect β-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of β-lactamases in a selected clinical isolate of A. baumannii of ST2P/ST195Ox and to characterize possible enzymes, as well as its β-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing conﬁrmed that the isolate harbored ﬁve bla gene alleles, namely, blaADC-73, blaTEM-1, blaOXA-23, blaOXA-58 and blaOXA-66, as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian A. baumannii AC30 genome and 76 regions with high homology. This study suggests that resistance to β-lactams in this A. baumannii isolate is mainly due to an overproduction of β-lactamases in combination with other resistance mechanism (efﬂux pump system).

Citovat

MLYNARČÍK, Patrik; Monika DOLEJSKÁ; Iva VAGNEROVA; Jana PETRZELOVA; Iva SUKKAR; Veronika ZDARSKA a Milan KOLAR. Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic. Life. 2021, roč. 11, č. 10, s. 1-14. ISSN 2075-1729. Dostupné z: https://doi.org/10.3390/life11101079.

@article{1836385,
   author = {Mlynarčík, Patrik and Dolejská, Monika and Vagnerova, Iva and Petrzelova, Jana and Sukkar, Iva and Zdarska, Veronika and Kolar, Milan},
   article_number = {10},
   doi = {https://doi.org/10.3390/life11101079},
   keywords = {beta-lactamse, antiibotic resistance, PCR, Acinetobacter baumannii, bacteria},
   language = {eng},
   issn = {2075-1729},
   journal = {Life},
   note = {Bez afiliace k MU.},
   title = {Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic},
   url = {https://www.mdpi.com/2075-1729/11/10/1079},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1836385
AU  - Mlynarčík, Patrik - Dolejská, Monika - Vagnerova, Iva - Petrzelova, Jana - Sukkar, Iva - Zdarska, Veronika - Kolar, Milan
PY  - 2021
TI  - Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic
JF  - Life
VL  - 11
IS  - 10
SP  - 1-14
EP  - 1-14
SN  - 20751729
N1  - Bez afiliace k MU.
KW  - beta-lactamse, antiibotic resistance, PCR, Acinetobacter baumannii, bacteria
UR  - https://www.mdpi.com/2075-1729/11/10/1079
N2  - Increasing antimicrobial resistance in nosocomial pathogens, such as Acinetobacter bauman- nii, is becoming a serious threat to public health. It is necessary to detect β-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of β-lactamases in a selected clinical isolate of A. baumannii of ST2P/ST195Ox and to characterize possible enzymes, as well as its β-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing conﬁrmed that the isolate harbored ﬁve bla gene alleles, namely, blaADC-73, blaTEM-1, blaOXA-23, blaOXA-58 and blaOXA-66, as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian A. baumannii AC30 genome and 76 regions with high homology. This study suggests that resistance to β-lactams in this A. baumannii isolate is mainly due to an overproduction of β-lactamases in combination with other resistance mechanism (efﬂux pump system).
ER  -

MLYNARČÍK, Patrik; Monika DOLEJSKÁ; Iva VAGNEROVA; Jana PETRZELOVA; Iva SUKKAR; Veronika ZDARSKA a Milan KOLAR. Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic. \textit{Life}. 2021, roč.~11, č.~10, s.~1-14. ISSN~2075-1729. Dostupné z: https://doi.org/10.3390/life11101079.

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