Limited light intensity and low temperature: Can plants survive
freezing in light conditions that more accurately replicate the
cold season in temperate regions?

J 2021

Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions?

NOVAK, J.; M. CERNY; J. ROIGNANT; Jan SKALÁK; I. SAIZ-FERNANDEZ et al.

Základní údaje

Originální název

Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions?

Autoři

NOVAK, J.; M. CERNY; J. ROIGNANT; Jan SKALÁK; I. SAIZ-FERNANDEZ; M. LUKLOVA; P. SKALAKOVA; V. ONDRISKOVA; O. NOVAK; A. PENCIK; D. TARKOWSKA; M. KAMENIAROVA; M. KARADY; R. VANKOVA a B. BRZOBOHATY

Vydání

Environmental and Experimental Botany, OXFORD, Elsevier, 2021, 0098-8472

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.028

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/21:00124281

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Arabidopsis thaliana; Cold; Acclimation; Freezing stress; Light; Proteome; Metabolome; Cytokinin; Low PPFD

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 2. 2022 16:16, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Plants in temperate regions have evolved mechanisms that enable them to survive sudden temperature drops. Experiments with plants grown in long-day conditions, in which they are most sensitive to freezing stress, indicate that the cold acclimation mechanism is light-dependent and does not fully operate under low light intensity. However, winter annuals like Arabidopsis thaliana Col-0 germinate in the fall, overwinter as rosettes, and thus must acclimate under short photoperiods and low irradiance. Thus, we have analysed effects of variations in light intensity in plants grown under short-day photoperiod at the 1.14 growth stage (14 rosette leaves). Plants were acclimated at 4 degrees C for seven days under control and limited-light conditions: 100 and 20 mu mol m-2s-1 photosynthetic photon flux density (PPFD), respectively. All cold-acclimated plants accumulated molecular markers reportedly associated with acquired freezing tolerance, including proline, sucrose, cold-responsive gene transcripts, dehydrins and low temperature-induced proteins. Observed changes (and similarity of freezing stress survival rates of plants in both light conditions) indicate that low PPFD did not inhibit the cold acclimation process. The molecular analysis identified distinct PPFD-specific adaptation mechanisms manifested in contrasting contents of anthocyanins, cytokinin conjugates, photosystem proteins, and enzymes involved in protein, energy, and reactive oxygen species metabolism. Finally, the results identify putative proteins and metabolite markers correlating with susceptibility to freezing stress of non-acclimated plants grown under low PPFD. Our data show that Arabidopsis plants grown under short-day photoperiods can be fully cold-acclimated under limited light conditions, employing standard and PPFD-specific pathways.

Návaznosti

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

NOVAK, J.; M. CERNY; J. ROIGNANT; Jan SKALÁK; I. SAIZ-FERNANDEZ; M. LUKLOVA; P. SKALAKOVA; V. ONDRISKOVA; O. NOVAK; A. PENCIK; D. TARKOWSKA; M. KAMENIAROVA; M. KARADY; R. VANKOVA a B. BRZOBOHATY. Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions? Environmental and Experimental Botany. OXFORD: Elsevier, 2021, roč. 190, OCT, s. 104581-104593. ISSN 0098-8472. Dostupné z: https://doi.org/10.1016/j.envexpbot.2021.104581.

@article{1836938,
   author = {Novak, J. and Cerny, M. and Roignant, J. and Skalák, Jan and SaizandFernandez, I. and Luklova, M. and Skalakova, P. and Ondriskova, V. and Novak, O. and Pencik, A. and Tarkowska, D. and Kameniarova, M. and Karady, M. and Vankova, R. and Brzobohaty, B.},
   article_location = {OXFORD},
   article_number = {OCT},
   doi = {https://doi.org/10.1016/j.envexpbot.2021.104581},
   keywords = {Arabidopsis thaliana; Cold; Acclimation; Freezing stress; Light; Proteome; Metabolome; Cytokinin; Low PPFD},
   language = {eng},
   issn = {0098-8472},
   journal = {Environmental and Experimental Botany},
   title = {Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions?},
   url = {https://www.sciencedirect.com/science/article/pii/S0098847221002112?via%3Dihub},
   volume = {190},
   year = {2021}
}

TY  - JOUR
ID  - 1836938
AU  - Novak, J. - Cerny, M. - Roignant, J. - Skalák, Jan - Saiz-Fernandez, I. - Luklova, M. - Skalakova, P. - Ondriskova, V. - Novak, O. - Pencik, A. - Tarkowska, D. - Kameniarova, M. - Karady, M. - Vankova, R. - Brzobohaty, B.
PY  - 2021
TI  - Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions?
JF  - Environmental and Experimental Botany
VL  - 190
IS  - OCT
SP  - 104581
EP  - 104581
PB  - Elsevier
SN  - 00988472
KW  - Arabidopsis thaliana
KW  - Cold
KW  - Acclimation
KW  - Freezing stress
KW  - Light
KW  - Proteome
KW  - Metabolome
KW  - Cytokinin
KW  - Low PPFD
UR  - https://www.sciencedirect.com/science/article/pii/S0098847221002112?via%3Dihub
N2  - Plants in temperate regions have evolved mechanisms that enable them to survive sudden temperature drops. Experiments with plants grown in long-day conditions, in which they are most sensitive to freezing stress, indicate that the cold acclimation mechanism is light-dependent and does not fully operate under low light intensity. However, winter annuals like Arabidopsis thaliana Col-0 germinate in the fall, overwinter as rosettes, and thus must acclimate under short photoperiods and low irradiance. Thus, we have analysed effects of variations in light intensity in plants grown under short-day photoperiod at the 1.14 growth stage (14 rosette leaves). Plants were acclimated at 4 degrees C for seven days under control and limited-light conditions: 100 and 20 mu mol m-2s-1 photosynthetic photon flux density (PPFD), respectively. All cold-acclimated plants accumulated molecular markers reportedly associated with acquired freezing tolerance, including proline, sucrose, cold-responsive gene transcripts, dehydrins and low temperature-induced proteins. Observed changes (and similarity of freezing stress survival rates of plants in both light conditions) indicate that low PPFD did not inhibit the cold acclimation process. The molecular analysis identified distinct PPFD-specific adaptation mechanisms manifested in contrasting contents of anthocyanins, cytokinin conjugates, photosystem proteins, and enzymes involved in protein, energy, and reactive oxygen species metabolism. Finally, the results identify putative proteins and metabolite markers correlating with susceptibility to freezing stress of non-acclimated plants grown under low PPFD. Our data show that Arabidopsis plants grown under short-day photoperiods can be fully cold-acclimated under limited light conditions, employing standard and PPFD-specific pathways.
ER  -

NOVAK, J.; M. CERNY; J. ROIGNANT; Jan SKALÁK; I. SAIZ-FERNANDEZ; M. LUKLOVA; P. SKALAKOVA; V. ONDRISKOVA; O. NOVAK; A. PENCIK; D. TARKOWSKA; M. KAMENIAROVA; M. KARADY; R. VANKOVA a B. BRZOBOHATY. Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions? \textit{Environmental and Experimental Botany}. OXFORD: Elsevier, 2021, roč.~190, OCT, s.~104581-104593. ISSN~0098-8472. Dostupné z: https://doi.org/10.1016/j.envexpbot.2021.104581.

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