Další formáty:
BibTeX
LaTeX
RIS
@article{1838040,
author = {Fajkus, Petr and Kilar, Agata Magdalena and Nelson, A.D.L. and Hola, M. and Peska, V. and Goffová, Ivana and Fojtová, Miloslava and Zachová, Dagmar and Fulnečková, Jana and Fajkus, Jiří},
article_location = {Oxford},
article_number = {13},
doi = {http://dx.doi.org/10.1093/nar/gkab545},
keywords = {TEMPLATE-BOUNDARY DEFINITION; SECONDARY STRUCTURE; POLYMERASE-III; SNRNA GENES; SEQUENCE; IDENTIFICATION; PROMOTER; PSEUDOKNOT; ELEMENT; ARABIDOPSIS},
language = {eng},
issn = {0305-1048},
journal = {Nucleic acids research},
title = {Evolution of plant telomerase RNAs: farther to the past, deeper to the roots},
url = {https://watermark.silverchair.com/gkab545.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAtYwggLSBgkqhkiG9w0BBwagggLDMIICvwIBADCCArgGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMJH7i4repl5kssFDwAgEQgIICic2a0sBtSCxRB0q2AJY0_-lH8kpqQpRwLwLA4PxPLpLSwC},
volume = {49},
year = {2021}
}
TY - JOUR
ID - 1838040
AU - Fajkus, Petr - Kilar, Agata Magdalena - Nelson, A.D.L. - Hola, M. - Peska, V. - Goffová, Ivana - Fojtová, Miloslava - Zachová, Dagmar - Fulnečková, Jana - Fajkus, Jiří
PY - 2021
TI - Evolution of plant telomerase RNAs: farther to the past, deeper to the roots
JF - Nucleic acids research
VL - 49
IS - 13
SP - 7680-7694
EP - 7680-7694
PB - Oxford University Press
SN - 03051048
KW - TEMPLATE-BOUNDARY DEFINITION
KW - SECONDARY STRUCTURE
KW - POLYMERASE-III
KW - SNRNA GENES
KW - SEQUENCE
KW - IDENTIFICATION
KW - PROMOTER
KW - PSEUDOKNOT
KW - ELEMENT
KW - ARABIDOPSIS
UR - https://watermark.silverchair.com/gkab545.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAtYwggLSBgkqhkiG9w0BBwagggLDMIICvwIBADCCArgGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMJH7i4repl5kssFDwAgEQgIICic2a0sBtSCxRB0q2AJY0_-lH8kpqQpRwLwLA4PxPLpLSwC
N2 - The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Homologous TRs were then used to build covariance models to identify TRs in more distant species. Transcripts of the identified TRs were confirmed by transcriptomic data, RT-PCR and Northern hybridization. A templating role for one of our candidates was validated in Physcomitrium patens. Analysis of secondary structure demonstrated a deep conservation of motifs (pseudoknot and template boundary element) observed in all published TRs. These results elucidate the evolution of the earliest eukaryotic TRs. linking the common origin of TRs across Diaphoretickes, and underlying evolutionary transitions in telomere repeats. [GRAPHICS] .
ER -
FAJKUS, Petr, Agata Magdalena KILAR, A.D.L. NELSON, M. HOLA, V. PESKA, Ivana GOFFOVÁ, Miloslava FOJTOVÁ, Dagmar ZACHOVÁ, Jana FULNEČKOVÁ a Jiří FAJKUS. Evolution of plant telomerase RNAs: farther to the past, deeper to the roots. Online. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2021, roč.~49, č.~13, s.~7680-7694. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkab545. [citováno 2024-04-23]
|
