A CENH3 mutation promotes meiotic exit and restores fertility
in SMG7-deficient Arabidopsis

J 2021

A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis

CAPITAO, C., Sorin TANASA, Jaroslav FULNEČEK, Vivek Kumar RAXWAL, S. AKIMCHEVA et. al.

Basic information

Original name

A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis

Authors

CAPITAO, C., Sorin TANASA (642 Romania, belonging to the institution), Jaroslav FULNEČEK (203 Czech Republic, belonging to the institution), Vivek Kumar RAXWAL (356 India, belonging to the institution), S. AKIMCHEVA, P. BULANKOVA, Pavlína MIKULKOVÁ (203 Czech Republic, belonging to the institution), Lucie CRHÁK KHAITOVÁ (203 Czech Republic, belonging to the institution), M. KALIDASS, I. LERMONTOVA, M. SCHEIDID and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLoS Genetics, San Francisco, Public Library of Science, 2021, 1553-7404

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.020

RIV identification code

RIV/00216224:14740/21:00124389

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pgen.1009779

UT WoS

000757101300002

Keywords in English

CHROMOSOME SEGREGATION; MITOTIC EXIT; MEIOSIS-I; GENE; PROGRESSION; HOMOLOG; SMG7; SPOROGENESIS; INITIATION; EVOLUTION

Abstract

V originále

Meiosis in angiosperm plants is followed by mitotic divisions to form multicellular haploid gametophytes. Termination of meiosis and transition to gametophytic development is, in Arabidopsis, governed by a dedicated mechanism that involves SMG7 and TDM1 proteins. Mutants carrying the smg7-6 allele are semi-fertile due to reduced pollen production. We found that instead of forming tetrads, smg7-6 pollen mother cells undergo multiple rounds of chromosome condensation and spindle assembly at the end of meiosis, resembling aberrant attempts to undergo additional meiotic divisions. A suppressor screen uncovered a mutation in centromeric histone H3 (CENH3) that increased fertility and promoted meiotic exit in smg7-6 plants. The mutation led to inefficient splicing of the CENH3 mRNA and a substantial decrease of CENH3, resulting in smaller centromeres. The reduced level of CENH3 delayed formation of the mitotic spindle but did not have an apparent effect on plant growth and development. We suggest that impaired spindle re-assembly at the end of meiosis limits aberrant divisions in smg7-6 plants and promotes formation of tetrads and viable pollen. Furthermore, the mutant with reduced level of CENH3 was very inefficient haploid inducer indicating that differences in centromere size is not the key determinant of centromere-mediated genome elimination.

Links

EF15_003/0000479, research and development project

Name: Regulace rostlinné meiózy

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

CAPITAO, C., Sorin TANASA, Jaroslav FULNEČEK, Vivek Kumar RAXWAL, S. AKIMCHEVA, P. BULANKOVA, Pavlína MIKULKOVÁ, Lucie CRHÁK KHAITOVÁ, M. KALIDASS, I. LERMONTOVA, M. SCHEIDID and Karel ŘÍHA. A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. PLoS Genetics. San Francisco: Public Library of Science, 2021, vol. 17, No 9, p. „e1009779“, 26 pp. ISSN 1553-7404. Available from: https://dx.doi.org/10.1371/journal.pgen.1009779.

@article{1840457,
   author = {Capitao, C. and Tanasa, Sorin and Fulneček, Jaroslav and Raxwal, Vivek Kumar and Akimcheva, S. and Bulankova, P. and Mikulková, Pavlína and Crhák Khaitová, Lucie and Kalidass, M. and Lermontova, I. and Scheidid, M. and Říha, Karel},
   article_location = {San Francisco},
   article_number = {9},
   doi = {http://dx.doi.org/10.1371/journal.pgen.1009779},
   keywords = {CHROMOSOME SEGREGATION; MITOTIC EXIT; MEIOSIS-I; GENE; PROGRESSION; HOMOLOG; SMG7; SPOROGENESIS; INITIATION; EVOLUTION},
   language = {eng},
   issn = {1553-7404},
   journal = {PLoS Genetics},
   title = {A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis},
   url = {https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1009779},
   volume = {17},
   year = {2021}
}

TY  - JOUR
ID  - 1840457
AU  - Capitao, C. - Tanasa, Sorin - Fulneček, Jaroslav - Raxwal, Vivek Kumar - Akimcheva, S. - Bulankova, P. - Mikulková, Pavlína - Crhák Khaitová, Lucie - Kalidass, M. - Lermontova, I. - Scheidid, M. - Říha, Karel
PY  - 2021
TI  - A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis
JF  - PLoS Genetics
VL  - 17
IS  - 9
SP  - „e1009779“
EP  - „e1009779“
PB  - Public Library of Science
SN  - 15537404
KW  - CHROMOSOME SEGREGATION
KW  - MITOTIC EXIT
KW  - MEIOSIS-I
KW  - GENE
KW  - PROGRESSION
KW  - HOMOLOG
KW  - SMG7
KW  - SPOROGENESIS
KW  - INITIATION
KW  - EVOLUTION
UR  - https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1009779
N2  - Meiosis in angiosperm plants is followed by mitotic divisions to form multicellular haploid gametophytes. Termination of meiosis and transition to gametophytic development is, in Arabidopsis, governed by a dedicated mechanism that involves SMG7 and TDM1 proteins. Mutants carrying the smg7-6 allele are semi-fertile due to reduced pollen production. We found that instead of forming tetrads, smg7-6 pollen mother cells undergo multiple rounds of chromosome condensation and spindle assembly at the end of meiosis, resembling aberrant attempts to undergo additional meiotic divisions. A suppressor screen uncovered a mutation in centromeric histone H3 (CENH3) that increased fertility and promoted meiotic exit in smg7-6 plants. The mutation led to inefficient splicing of the CENH3 mRNA and a substantial decrease of CENH3, resulting in smaller centromeres. The reduced level of CENH3 delayed formation of the mitotic spindle but did not have an apparent effect on plant growth and development. We suggest that impaired spindle re-assembly at the end of meiosis limits aberrant divisions in smg7-6 plants and promotes formation of tetrads and viable pollen. Furthermore, the mutant with reduced level of CENH3 was very inefficient haploid inducer indicating that differences in centromere size is not the key determinant of centromere-mediated genome elimination.
ER  -

CAPITAO, C., Sorin TANASA, Jaroslav FULNEČEK, Vivek Kumar RAXWAL, S. AKIMCHEVA, P. BULANKOVA, Pavlína MIKULKOVÁ, Lucie CRHÁK KHAITOVÁ, M. KALIDASS, I. LERMONTOVA, M. SCHEIDID and Karel ŘÍHA. A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. \textit{PLoS Genetics}. San Francisco: Public Library of Science, 2021, vol.~17, No~9, p.~„e1009779“, 26 pp. ISSN~1553-7404. Available from: https://dx.doi.org/10.1371/journal.pgen.1009779.

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