ESEM Methodology for the Study of Ice Samples at
Environmentally Relevant Subzero Temperatures:

J 2022

ESEM Methodology for the Study of Ice Samples at Environmentally Relevant Subzero Temperatures: "Subzero ESEM"

ZÁVACKÁ, Kamila; Vilém NEDĚLA; Eva TIHLAŘÍKOVÁ; Pavla ŠABACKÁ; Jiří MAXA et al.

Základní údaje

Originální název

ESEM Methodology for the Study of Ice Samples at Environmentally Relevant Subzero Temperatures: "Subzero ESEM"

Autoři

ZÁVACKÁ, Kamila; Vilém NEDĚLA; Eva TIHLAŘÍKOVÁ; Pavla ŠABACKÁ; Jiří MAXA a Dominik HEGER

Vydání

Microscopy and Microanalysis, Cambridge University Press, 2022, 1431-9276

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00119702

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

morphological study; static observation; "subzero ESEM"; temperature simulation; thermodynamic equilibrium

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 3. 2022 16:16, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Frozen aqueous solutions are an important subject of study in numerous scientific branches including the pharmaceutical and food industry, atmospheric chemistry, biology, and medicine. Here, we present an advanced environmental scanning electron microscope methodology for research of ice samples at environmentally relevant subzero temperatures, thus under conditions in which it is extremely challenging to maintain the thermodynamic equilibrium of the specimen. The methodology opens possibilities to observe intact ice samples at close to natural conditions. Based on the results of ANSYS software simulations of the surface temperature of a frozen sample, and knowledge of the partial pressure of water vapor in the gas mixture near the sample, we monitored static ice samples over several minutes. We also discuss possible artifacts that can arise from unwanted surface ice formation on, or ice sublimation from, the sample, as a consequence of shifting conditions away from thermodynamic equilibrium in the specimen chamber. To demonstrate the applicability of the methodology, we characterized how the true morphology of ice spheres containing salt changed upon aging and the morphology of ice spheres containing bovine serum albumin. After combining static observations with the dynamic process of ice sublimation from the sample, we can attain images with nanometer resolution.

Návaznosti

GA19-08239S, projekt VaV

Název: Spektroskopie a mikroskopie chemických látek v ledu pro environmentální a farmaceutické účely

Investor: Grantová agentura ČR, Spektroskopie a mikroskopie chemických látek v ledu pro environmentální a farmaceutické účely

Citovat

ZÁVACKÁ, Kamila; Vilém NEDĚLA; Eva TIHLAŘÍKOVÁ; Pavla ŠABACKÁ; Jiří MAXA a Dominik HEGER. ESEM Methodology for the Study of Ice Samples at Environmentally Relevant Subzero Temperatures: "Subzero ESEM". Microscopy and Microanalysis. Cambridge University Press, 2022, roč. 28, č. 1, s. 196-209. ISSN 1431-9276. Dostupné z: https://doi.org/10.1017/S1431927621013854.

@article{1840485,
   author = {Závacká, Kamila and Neděla, Vilém and Tihlaříková, Eva and Šabacká, Pavla and Maxa, Jiří and Heger, Dominik},
   article_number = {1},
   doi = {https://doi.org/10.1017/S1431927621013854},
   keywords = {morphological study; static observation; "subzero ESEM"; temperature simulation; thermodynamic equilibrium},
   language = {eng},
   issn = {1431-9276},
   journal = {Microscopy and Microanalysis},
   title = {ESEM Methodology for the Study of Ice Samples at Environmentally Relevant Subzero Temperatures: "Subzero ESEM"},
   url = {https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/esem-methodology-for-the-study-of-ice-samples-at-environmentally-relevant-subzero-temperatures-subzero-esem/0145A6ED83A07396E5FA3E7655DEF66D},
   volume = {28},
   year = {2022}
}

TY  - JOUR
ID  - 1840485
AU  - Závacká, Kamila - Neděla, Vilém - Tihlaříková, Eva - Šabacká, Pavla - Maxa, Jiří - Heger, Dominik
PY  - 2022
TI  - ESEM Methodology for the Study of Ice Samples at Environmentally Relevant Subzero Temperatures: "Subzero ESEM"
JF  - Microscopy and Microanalysis
VL  - 28
IS  - 1
SP  - 196-209
EP  - 196-209
PB  - Cambridge University Press
SN  - 14319276
KW  - morphological study
KW  - static observation
KW  - "subzero ESEM"
KW  - temperature simulation
KW  - thermodynamic equilibrium
UR  - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/esem-methodology-for-the-study-of-ice-samples-at-environmentally-relevant-subzero-temperatures-subzero-esem/0145A6ED83A07396E5FA3E7655DEF66D
N2  - Frozen aqueous solutions are an important subject of study in numerous scientific branches including the pharmaceutical and food industry, atmospheric chemistry, biology, and medicine. Here, we present an advanced environmental scanning electron microscope methodology for research of ice samples at environmentally relevant subzero temperatures, thus under conditions in which it is extremely challenging to maintain the thermodynamic equilibrium of the specimen. The methodology opens possibilities to observe intact ice samples at close to natural conditions. Based on the results of ANSYS software simulations of the surface temperature of a frozen sample, and knowledge of the partial pressure of water vapor in the gas mixture near the sample, we monitored static ice samples over several minutes. We also discuss possible artifacts that can arise from unwanted surface ice formation on, or ice sublimation from, the sample, as a consequence of shifting conditions away from thermodynamic equilibrium in the specimen chamber. To demonstrate the applicability of the methodology, we characterized how the true morphology of ice spheres containing salt changed upon aging and the morphology of ice spheres containing bovine serum albumin. After combining static observations with the dynamic process of ice sublimation from the sample, we can attain images with nanometer resolution.
ER  -

ZÁVACKÁ, Kamila; Vilém NEDĚLA; Eva TIHLAŘÍKOVÁ; Pavla ŠABACKÁ; Jiří MAXA a Dominik HEGER. ESEM Methodology for the Study of Ice Samples at Environmentally Relevant Subzero Temperatures: ''Subzero ESEM''. \textit{Microscopy and Microanalysis}. Cambridge University Press, 2022, roč.~28, č.~1, s.~196-209. ISSN~1431-9276. Dostupné z: https://doi.org/10.1017/S1431927621013854.

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