Conservation and over-representation of G-quadruplex sequences
in regulatory regions of mitochondrial DNA across distinct
taxonomic sub-groups

J 2022

Conservation and over-representation of G-quadruplex sequences in regulatory regions of mitochondrial DNA across distinct taxonomic sub-groups

BOHÁLOVÁ, Natália; Michaela DOBROVOLNÁ; Václav BRÁZDA a Stefan BIDULA

Základní údaje

Originální název

Conservation and over-representation of G-quadruplex sequences in regulatory regions of mitochondrial DNA across distinct taxonomic sub-groups

Autoři

BOHÁLOVÁ, Natália; Michaela DOBROVOLNÁ; Václav BRÁZDA a Stefan BIDULA

Vydání

Biochimie, Elsevier, 2022, 0300-9084

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00125545

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Mitochondria; Genome; G-quadruplex; Evolution; D-loop

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 5. 2022 13:27, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

G-quadruplexes have important regulatory roles in the nuclear genome but their distribution and potential roles in mitochondrial DNA (mtDNA) are poorly understood. We analysed 11883 mtDNA sequences from 18 taxonomic sub-groups and identified their frequency and location within mtDNA. Large differences in both the frequency and number of putative quadruplex-forming sequences (PQS) were observed amongst all the organisms and PQS frequency was negatively correlated with an increase in evolutionary age. PQS were over-represented in the 3′UTRs, D-loops, replication origins, and stem loops, indicating regulatory roles for quadruplexes in mtDNA. Variations of the G-quadruplex-forming sequence in the conserved sequence block II (CSBII) region of the human D-loop were conserved amongst other mammals, amphibians, birds, reptiles, and fishes. This D-loop PQS was conserved in the duplicated control regions of some birds and reptiles, indicating its importance to mitochondrial function. The guanine tracts in these PQS also displayed significant length heterogeneity and the length of these guanine tracts were generally longest in bird mtDNA. This information provides further insights into how G4s may contribute to the regulation and function of mtDNA and acts as a database of information for future studies investigating mitochondrial G4s in organisms other than humans.

Citovat

BOHÁLOVÁ, Natália; Michaela DOBROVOLNÁ; Václav BRÁZDA a Stefan BIDULA. Conservation and over-representation of G-quadruplex sequences in regulatory regions of mitochondrial DNA across distinct taxonomic sub-groups. Biochimie. Elsevier, 2022, roč. 194, March, s. 28-34. ISSN 0300-9084. Dostupné z: https://doi.org/10.1016/j.biochi.2021.12.006.

@article{1842099,
   author = {Bohálová, Natália and Dobrovolná, Michaela and Brázda, Václav and Bidula, Stefan},
   article_number = {March},
   doi = {https://doi.org/10.1016/j.biochi.2021.12.006},
   keywords = {Mitochondria; Genome; G-quadruplex; Evolution; D-loop},
   language = {eng},
   issn = {0300-9084},
   journal = {Biochimie},
   title = {Conservation and over-representation of G-quadruplex sequences in regulatory regions of mitochondrial DNA across distinct taxonomic sub-groups},
   url = {https://www.sciencedirect.com/science/article/pii/S0300908421002947},
   volume = {194},
   year = {2022}
}

TY  - JOUR
ID  - 1842099
AU  - Bohálová, Natália - Dobrovolná, Michaela - Brázda, Václav - Bidula, Stefan
PY  - 2022
TI  - Conservation and over-representation of G-quadruplex sequences in regulatory regions of mitochondrial DNA across distinct taxonomic sub-groups
JF  - Biochimie
VL  - 194
IS  - March
SP  - 28-34
EP  - 28-34
PB  - Elsevier
SN  - 03009084
KW  - Mitochondria
KW  - Genome
KW  - G-quadruplex
KW  - Evolution
KW  - D-loop
UR  - https://www.sciencedirect.com/science/article/pii/S0300908421002947
N2  - G-quadruplexes have important regulatory roles in the nuclear genome but their distribution and potential roles in mitochondrial DNA (mtDNA) are poorly understood. We analysed 11883 mtDNA sequences from 18 taxonomic sub-groups and identified their frequency and location within mtDNA. Large differences in both the frequency and number of putative quadruplex-forming sequences (PQS) were observed amongst all the organisms and PQS frequency was negatively correlated with an increase in evolutionary age. PQS were over-represented in the 3′UTRs, D-loops, replication origins, and stem loops, indicating regulatory roles for quadruplexes in mtDNA. Variations of the G-quadruplex-forming sequence in the conserved sequence block II (CSBII) region of the human D-loop were conserved amongst other mammals, amphibians, birds, reptiles, and fishes. This D-loop PQS was conserved in the duplicated control regions of some birds and reptiles, indicating its importance to mitochondrial function. The guanine tracts in these PQS also displayed significant length heterogeneity and the length of these guanine tracts were generally longest in bird mtDNA. This information provides further insights into how G4s may contribute to the regulation and function of mtDNA and acts as a database of information for future studies investigating mitochondrial G4s in organisms other than humans.
ER  -

BOHÁLOVÁ, Natália; Michaela DOBROVOLNÁ; Václav BRÁZDA a Stefan BIDULA. Conservation and over-representation of G-quadruplex sequences in regulatory regions of mitochondrial DNA across distinct taxonomic sub-groups. \textit{Biochimie}. Elsevier, 2022, roč.~194, March, s.~28-34. ISSN~0300-9084. Dostupné z: https://doi.org/10.1016/j.biochi.2021.12.006.

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