Impact of Chromate and Dichromate on Lysozyme Stability: A
Spectroscopic and Molecular Docking Investigation

J 2022

Impact of Chromate and Dichromate on Lysozyme Stability: A Spectroscopic and Molecular Docking Investigation

SUBADINI, Suchismita; Ram Sankar PANIGRAHY; Nishesh Kumar GUPTA; Krishnendu BERA; Harekrushna SAHOO et al.

Základní údaje

Originální název

Impact of Chromate and Dichromate on Lysozyme Stability: A Spectroscopic and Molecular Docking Investigation

Autoři

SUBADINI, Suchismita; Ram Sankar PANIGRAHY; Nishesh Kumar GUPTA; Krishnendu BERA a Harekrushna SAHOO

Vydání

Luminescence, Wiley, 2022, 1522-7235

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/22:00125554

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Lyz; fluorescence quenching; anisotropy; chromate; dichromate

Štítky

143160, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 1. 2023 16:32, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

A comparative study of interaction between Lysozyme (Lyz) with two hexavalent chromate ions; chromate and dichromate; which are prevalently known for their toxicity, was investigated using different spectroscopic techniques along with molecular docking study. Both steady-state and time-resolved studies revealed that the addition of chromate/dichromate is responsible for strong quenching of intrinsic fluorescence in Lyz and the quenching is caused by both static and dynamic quenching mechanism. Different binding and thermodynamic parameters were also calculated at different temperatures from the intrinsic fluorescence of Lyz. The conformational change in Lyz and thermodynamic parameters obtained during the course of interaction with chromate/dichromate were well-supported by the molecular docking results.

Návaznosti

GF20-05789L, projekt VaV

Název: Charakterizace přirozeně neuspořádaných proteinů

Investor: Grantová agentura ČR, Characterization of intrinsically disordered proteins, Partnerská agentura (Rakousko)

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

Citovat

SUBADINI, Suchismita; Ram Sankar PANIGRAHY; Nishesh Kumar GUPTA; Krishnendu BERA a Harekrushna SAHOO. Impact of Chromate and Dichromate on Lysozyme Stability: A Spectroscopic and Molecular Docking Investigation. Luminescence. Wiley, 2022, roč. 37, č. 6, s. 876-882. ISSN 1522-7235. Dostupné z: https://doi.org/10.1002/bio.4231.

@article{1842397,
   author = {Subadini, Suchismita and Panigrahy, Ram Sankar and Gupta, Nishesh Kumar and Bera, Krishnendu and Sahoo, Harekrushna},
   article_number = {6},
   doi = {https://doi.org/10.1002/bio.4231},
   keywords = {Lyz; fluorescence quenching; anisotropy; chromate; dichromate},
   language = {eng},
   issn = {1522-7235},
   journal = {Luminescence},
   title = {Impact of Chromate and Dichromate on Lysozyme Stability: A Spectroscopic and Molecular Docking Investigation},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/bio.4231},
   volume = {37},
   year = {2022}
}

TY  - JOUR
ID  - 1842397
AU  - Subadini, Suchismita - Panigrahy, Ram Sankar - Gupta, Nishesh Kumar - Bera, Krishnendu - Sahoo, Harekrushna
PY  - 2022
TI  - Impact of Chromate and Dichromate on Lysozyme Stability: A Spectroscopic and Molecular Docking Investigation
JF  - Luminescence
VL  - 37
IS  - 6
SP  - 876-882
EP  - 876-882
PB  - Wiley
SN  - 15227235
KW  - Lyz
KW  - fluorescence quenching
KW  - anisotropy
KW  - chromate
KW  - dichromate
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/bio.4231
N2  - A comparative study of interaction between Lysozyme (Lyz) with two hexavalent chromate ions; chromate and dichromate; which are prevalently known for their toxicity, was investigated using different spectroscopic techniques along with molecular docking study. Both steady-state and time-resolved studies revealed that the addition of chromate/dichromate is responsible for strong quenching of intrinsic fluorescence in Lyz and the quenching is caused by both static and dynamic quenching mechanism. Different binding and thermodynamic parameters were also calculated at different temperatures from the intrinsic fluorescence of Lyz. The conformational change in Lyz and thermodynamic parameters obtained during the course of interaction with chromate/dichromate were well-supported by the molecular docking results.
ER  -

SUBADINI, Suchismita; Ram Sankar PANIGRAHY; Nishesh Kumar GUPTA; Krishnendu BERA a Harekrushna SAHOO. Impact of Chromate and Dichromate on Lysozyme Stability: A Spectroscopic and Molecular Docking Investigation. \textit{Luminescence}. Wiley, 2022, roč.~37, č.~6, s.~876-882. ISSN~1522-7235. Dostupné z: https://doi.org/10.1002/bio.4231.

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