Characterisation of Waterborne Psychrophilic Massilia Isolates
with Violacein Production and Description of Massilia
antarctica sp. nov.

J 2022

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Hans-Jürgen BUSSE, Vendula KOUBLOVÁ, Stanislava KRÁLOVÁ et. al.

Základní údaje

Originální název

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

Autoři

SEDLÁČEK, Ivo (203 Česká republika, garant, domácí), Pavla HOLOCHOVÁ (203 Česká republika, domácí), Hans-Jürgen BUSSE, Vendula KOUBLOVÁ (203 Česká republika, domácí), Stanislava KRÁLOVÁ (703 Slovensko, domácí), Pavel ŠVEC (203 Česká republika, domácí), Roman SOBOTKA, Eva STAŇKOVÁ (203 Česká republika, domácí), Jan PILNÝ, Ondrej ŠEDO (203 Česká republika, domácí), Jana SMOLÍKOVÁ a Karel SEDLÁŘ

Vydání

Microorganisms, MDPI, 2022, 2076-2607

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.500

Kód RIV

RIV/00216224:14310/22:00125595

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/microorganisms10040704

UT WoS

000786017300001

Klíčová slova anglicky

Massilia; violacein; psychrophilic; description; whole-genome sequencing; Antarctica

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 15:01, Ing. Martina Blahová

Anotace

V originále

A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398T were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398T (= CCM 8941T = LMG 32108T).

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Hans-Jürgen BUSSE, Vendula KOUBLOVÁ, Stanislava KRÁLOVÁ, Pavel ŠVEC, Roman SOBOTKA, Eva STAŇKOVÁ, Jan PILNÝ, Ondrej ŠEDO, Jana SMOLÍKOVÁ a Karel SEDLÁŘ. Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. Microorganisms. MDPI, 2022, roč. 10, č. 4, s. 1-14. ISSN 2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms10040704.

@article{1843919,
   author = {Sedláček, Ivo and Holochová, Pavla and Busse, HansandJürgen and Koublová, Vendula and Králová, Stanislava and Švec, Pavel and Sobotka, Roman and Staňková, Eva and Pilný, Jan and Šedo, Ondrej and Smolíková, Jana and Sedlář, Karel},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/microorganisms10040704},
   keywords = {Massilia; violacein; psychrophilic; description; whole-genome sequencing; Antarctica},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.},
   url = {https://www.mdpi.com/2076-2607/10/4/704},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1843919
AU  - Sedláček, Ivo - Holochová, Pavla - Busse, Hans-Jürgen - Koublová, Vendula - Králová, Stanislava - Švec, Pavel - Sobotka, Roman - Staňková, Eva - Pilný, Jan - Šedo, Ondrej - Smolíková, Jana - Sedlář, Karel
PY  - 2022
TI  - Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.
JF  - Microorganisms
VL  - 10
IS  - 4
SP  - 1-14
EP  - 1-14
PB  - MDPI
SN  - 20762607
KW  - Massilia
KW  - violacein
KW  - psychrophilic
KW  - description
KW  - whole-genome sequencing
KW  - Antarctica
UR  - https://www.mdpi.com/2076-2607/10/4/704
N2  - A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398T were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398T (= CCM 8941T = LMG 32108T).
ER  -

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Hans-Jürgen BUSSE, Vendula KOUBLOVÁ, Stanislava KRÁLOVÁ, Pavel ŠVEC, Roman SOBOTKA, Eva STAŇKOVÁ, Jan PILNÝ, Ondrej ŠEDO, Jana SMOLÍKOVÁ a Karel SEDLÁŘ. Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. \textit{Microorganisms}. MDPI, 2022, roč.~10, č.~4, s.~1-14. ISSN~2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms10040704.

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