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@article{1845339,
author = {Shapoval, Oleksandr and Brandmeier, Julian and Nahorniak, Mykhailo and Oleksa, Viktoriia and Makhneva, Ekaterina and Gorris, HansandHeiner and Farka, Zdeněk and Horák, Daniel},
article_number = {123400},
doi = {http://dx.doi.org/10.1016/j.talanta.2022.123400},
keywords = {Photon-upconversion nanoparticle; Poly(methyl vinyl ether‐alt‐maleic acid);Bioconjugation; Immunoassay; Upconversion-linked immunosorbent assay; Cardiac troponin I},
language = {eng},
issn = {0039-9140},
journal = {Talanta},
title = {PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin},
url = {https://www.sciencedirect.com/science/article/pii/S0039914022001965},
volume = {244},
year = {2022}
}
TY - JOUR
ID - 1845339
AU - Shapoval, Oleksandr - Brandmeier, Julian - Nahorniak, Mykhailo - Oleksa, Viktoriia - Makhneva, Ekaterina - Gorris, Hans-Heiner - Farka, Zdeněk - Horák, Daniel
PY - 2022
TI - PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin
JF - Talanta
VL - 244
IS - 123400
SP - 1-8
EP - 1-8
PB - Elsevier
SN - 00399140
KW - Photon-upconversion nanoparticle
KW - Poly(methyl vinyl ether‐alt‐maleic acid);Bioconjugation
KW - Immunoassay
KW - Upconversion-linked immunosorbent assay
KW - Cardiac troponin I
UR - https://www.sciencedirect.com/science/article/pii/S0039914022001965
N2 - Surface engineering of upconverting nanoparticles (UCNPs) is crucial for their bioanalytical applications. Here, an antibody specific to cardiac troponin I (cTnI), an important biomarker for acute myocardial infection, was covalently immobilized on the surface of UCNPs to prepare a label for the detection of cTnI biomarker in an upconversion-linked immunoassay (ULISA). Core-shell UCNPs (NaYF4:Yb,Tm@NaYF4) were first coated with poly(methyl vinyl ether‐alt‐maleic acid) (PMVEMA) and then conjugated to antibodies. The morphology (size and uniformity), hydrodynamic diameter, chemical composition, and amount of coating on the of UCNPs, as well as their upconversion luminescence, colloidal stability, and leaching of Y3+ ions into the surrounding media, were determined. The developed ULISA allowed reaching a limit of detection (LOD) of 0.13 ng/ml and 0.25 ng/ml of cTnI in plasma and serum, respectively, which represents 12- and 2-fold improvement to conventional enzyme-linked immunosorbent based on the same immunoreagents.
ER -
SHAPOVAL, Oleksandr, Julian BRANDMEIER, Mykhailo NAHORNIAK, Viktoriia OLEKSA, Ekaterina MAKHNEVA, Hans-Heiner GORRIS, Zdeněk FARKA a Daniel HORÁK. PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin. \textit{Talanta}. Elsevier, 2022, roč.~244, č.~123400, s.~1-8. ISSN~0039-9140. Dostupné z: https://dx.doi.org/10.1016/j.talanta.2022.123400.
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