Chimeric Cellobiose Dehydrogenases Reveal the Function of
Cytochrome Domain Mobility for the Electron Transfer to Lytic
Polysaccharide Monooxygenase

J 2021

Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase

FELICE, A.K.G., C. SCHUSTER, A. KADEK, F. FILANDR, C.V. F. P. LAURENT et. al.

Základní údaje

Originální název

Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase

Autoři

FELICE, A.K.G., C. SCHUSTER, A. KADEK, F. FILANDR, C.V. F. P. LAURENT, S. SCHEIBLBRANDNER, L. SCHWAIGER, F. SCHACHINGER, D. KRACHER, C. SYGMUND, P. MAN, P. HALADA, C. OOSTENBRINK a R. LUDWIG

Vydání

ACS CATALYSIS, 2021, 2155-5435

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 13.700

Kód RIV

RIV/00216224:14740/21:00124506

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/acscatal.0c05294

UT WoS

000611450000005

Klíčová slova anglicky

cellobiose dehydrogenasechimeric enzymedomain swapping electron transferlytic polysaccharide monooxygenase

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 5. 2022 15:04, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The natural function of cellobiose dehydrogenase (CDH) to donate electrons from its catalytic flavodehydrogenase (DH) domain via its cytochrome (CYT) domain to lytic polysaccharide monooxygenase (LPMO) is an example of a highly efficient extracellular electron transfer chain. To investigate the function of the CYT domain movement in the two occurring electron transfer steps, two CDHs from the ascomycete Neurospora crassa (NcCDHIIA and NcCDHIIB) and five chimeric CDH enzymes created by domain swapping were studied in combination with the fungus' own LPMOs (NcLPMO9C and NcLPMO9F). Kinetic and electrochemical methods and hydrogen/deuterium exchange mass spectrometry were used to study the domain movement, interaction, and electron transfer kinetics. Molecular docking provided insights into the protein-protein interface, the orientation of domains, and binding energies. We find that the first, interdomain electron transfer step from the catalytic site in the DH domain to the CYT domain depends on steric and electrostatic interface complementarity and the length of the protein linker between both domains but not on the redox potential difference between the FAD and heme b cofactors. After CYT reduction, a conformational change of CDH from its closed state to an open state allows the second, interprotein electron transfer (IPET) step from CYT to LPMO to occur by direct interaction of the b-type heme and the type-2 copper center. Chimeric CDH enzymes favor the open state and achieve higher IPET rates by exposing the heme b cofactor to LPMO. The IPET, which is influenced by interface complementarity and the heme b redox potential, is very efficient with bimolecular rates between 2.9 x 10(5) and 1.1 x 10(6) M-1 s(-1).

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

FELICE, A.K.G., C. SCHUSTER, A. KADEK, F. FILANDR, C.V. F. P. LAURENT, S. SCHEIBLBRANDNER, L. SCHWAIGER, F. SCHACHINGER, D. KRACHER, C. SYGMUND, P. MAN, P. HALADA, C. OOSTENBRINK a R. LUDWIG. Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase. ACS CATALYSIS. 2021, roč. 11, č. 2, s. 517-532. ISSN 2155-5435. Dostupné z: https://dx.doi.org/10.1021/acscatal.0c05294.

@article{1845733,
   author = {Felice, A.K.G. and Schuster, C. and Kadek, A. and Filandr, F. and Laurent, C.V. F. P. and Scheiblbrandner, S. and Schwaiger, L. and Schachinger, F. and Kracher, D. and Sygmund, C. and Man, P. and Halada, P. and Oostenbrink, C. and Ludwig, R.},
   article_number = {2},
   doi = {http://dx.doi.org/10.1021/acscatal.0c05294},
   keywords = {cellobiose dehydrogenasechimeric enzymedomain swapping electron transferlytic polysaccharide monooxygenase},
   language = {eng},
   issn = {2155-5435},
   journal = {ACS CATALYSIS},
   title = {Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase},
   url = {https://pubs.acs.org/doi/10.1021/acscatal.0c05294},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1845733
AU  - Felice, A.K.G. - Schuster, C. - Kadek, A. - Filandr, F. - Laurent, C.V. F. P. - Scheiblbrandner, S. - Schwaiger, L. - Schachinger, F. - Kracher, D. - Sygmund, C. - Man, P. - Halada, P. - Oostenbrink, C. - Ludwig, R.
PY  - 2021
TI  - Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase
JF  - ACS CATALYSIS
VL  - 11
IS  - 2
SP  - 517-532
EP  - 517-532
SN  - 21555435
KW  - cellobiose dehydrogenasechimeric enzymedomain swapping electron transferlytic polysaccharide monooxygenase
UR  - https://pubs.acs.org/doi/10.1021/acscatal.0c05294
N2  - The natural function of cellobiose dehydrogenase (CDH) to donate electrons from its catalytic flavodehydrogenase (DH) domain via its cytochrome (CYT) domain to lytic polysaccharide monooxygenase (LPMO) is an example of a highly efficient extracellular electron transfer chain. To investigate the function of the CYT domain movement in the two occurring electron transfer steps, two CDHs from the ascomycete Neurospora crassa (NcCDHIIA and NcCDHIIB) and five chimeric CDH enzymes created by domain swapping were studied in combination with the fungus' own LPMOs (NcLPMO9C and NcLPMO9F). Kinetic and electrochemical methods and hydrogen/deuterium exchange mass spectrometry were used to study the domain movement, interaction, and electron transfer kinetics. Molecular docking provided insights into the protein-protein interface, the orientation of domains, and binding energies. We find that the first, interdomain electron transfer step from the catalytic site in the DH domain to the CYT domain depends on steric and electrostatic interface complementarity and the length of the protein linker between both domains but not on the redox potential difference between the FAD and heme b cofactors. After CYT reduction, a conformational change of CDH from its closed state to an open state allows the second, interprotein electron transfer (IPET) step from CYT to LPMO to occur by direct interaction of the b-type heme and the type-2 copper center. Chimeric CDH enzymes favor the open state and achieve higher IPET rates by exposing the heme b cofactor to LPMO. The IPET, which is influenced by interface complementarity and the heme b redox potential, is very efficient with bimolecular rates between 2.9 x 10(5) and 1.1 x 10(6) M-1 s(-1).
ER  -

FELICE, A.K.G., C. SCHUSTER, A. KADEK, F. FILANDR, C.V. F. P. LAURENT, S. SCHEIBLBRANDNER, L. SCHWAIGER, F. SCHACHINGER, D. KRACHER, C. SYGMUND, P. MAN, P. HALADA, C. OOSTENBRINK a R. LUDWIG. Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase. \textit{ACS CATALYSIS}. 2021, roč.~11, č.~2, s.~517-532. ISSN~2155-5435. Dostupné z: https://dx.doi.org/10.1021/acscatal.0c05294.

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