Selective Enhancement of the Cell-Permeabilizing Activity of
Adenylate Cyclase Toxin Does Not Increase Virulence of
Bordetella pertussis

J 2021

Selective Enhancement of the Cell-Permeabilizing Activity of Adenylate Cyclase Toxin Does Not Increase Virulence of Bordetella pertussis

HOLUBOVA, J., A. JUHASZ, J. MASIN, O. STANEK, D. JURNECKA et. al.

Základní údaje

Originální název

Selective Enhancement of the Cell-Permeabilizing Activity of Adenylate Cyclase Toxin Does Not Increase Virulence of Bordetella pertussis

Autoři

HOLUBOVA, J., A. JUHASZ, J. MASIN, O. STANEK, D. JURNECKA, A. OSICKOVA, P. SEBO a R. OSICKA

Vydání

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.208

Kód RIV

RIV/00216224:14740/21:00124529

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/ijms222111655

UT WoS

000726625300001

Klíčová slova anglicky

adenylate cyclase toxinBordetella pertussiscAMP intoxicationlung colonizationlung inflammationpore-forming activityRTX toxinvirulence

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 5. 2022 15:06, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The whooping cough agent, Bordetella pertussis, secretes an adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) that catalyzes the conversion of intracellular ATP to cAMP and through its signaling annihilates the bactericidal activities of host sentinel phagocytes. In parallel, CyaA permeabilizes host cells by the formation of cation-selective membrane pores that account for the hemolytic activity of CyaA. The pore-forming activity contributes to the overall cytotoxic effect of CyaA in vitro, and it has previously been proposed to synergize with the cAMP-elevating activity in conferring full virulence on B. pertussis in the mouse model of pneumonic infection. CyaA primarily targets myeloid phagocytes through binding of their complement receptor 3 (CR3, integrin alpha(M)beta(2), or CD11b/CD18). However, with a reduced efficacy, the toxin can promiscuously penetrate and permeabilize the cell membrane of a variety of non-myeloid cells that lack CR3 on the cell surface, including airway epithelial cells or erythrocytes, and detectably intoxicates them by cAMP. Here, we used CyaA variants with strongly and selectively enhanced or reduced pore-forming activity that, at the same time, exhibited a full capacity to elevate cAMP concentrations in both CR3-expressing and CR3-non-expressing target cells. Using B. pertussis mutants secreting such CyaA variants, we show that a selective enhancement of the cell-permeabilizing activity of CyaA does not increase the overall virulence and lethality of pneumonic B. pertussis infection of mice any further. In turn, a reduction of the cell-permeabilizing activity of CyaA did not reduce B. pertussis virulence any importantly. These results suggest that the phagocyte-paralyzing cAMP-elevating capacity of CyaA prevails over the cell-permeabilizing activity of CyaA that appears to play an auxiliary role in the biological activity of the CyaA toxin in the course of B. pertussis infections in vivo.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

HOLUBOVA, J., A. JUHASZ, J. MASIN, O. STANEK, D. JURNECKA, A. OSICKOVA, P. SEBO a R. OSICKA. Selective Enhancement of the Cell-Permeabilizing Activity of Adenylate Cyclase Toxin Does Not Increase Virulence of Bordetella pertussis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2021, roč. 22, č. 21, s. 11655-11672. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms222111655.

@article{1846839,
   author = {Holubova, J. and Juhasz, A. and Masin, J. and Stanek, O. and Jurnecka, D. and Osickova, A. and Sebo, P. and Osicka, R.},
   article_number = {21},
   doi = {http://dx.doi.org/10.3390/ijms222111655},
   keywords = {adenylate cyclase toxinBordetella pertussiscAMP intoxicationlung colonizationlung inflammationpore-forming activityRTX toxinvirulence},
   language = {eng},
   issn = {1422-0067},
   journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
   title = {Selective Enhancement of the Cell-Permeabilizing Activity of Adenylate Cyclase Toxin Does Not Increase Virulence of Bordetella pertussis},
   url = {https://www.mdpi.com/1422-0067/22/21/11655},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1846839
AU  - Holubova, J. - Juhasz, A. - Masin, J. - Stanek, O. - Jurnecka, D. - Osickova, A. - Sebo, P. - Osicka, R.
PY  - 2021
TI  - Selective Enhancement of the Cell-Permeabilizing Activity of Adenylate Cyclase Toxin Does Not Increase Virulence of Bordetella pertussis
JF  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
VL  - 22
IS  - 21
SP  - 11655
EP  - 11655
SN  - 14220067
KW  - adenylate cyclase toxinBordetella pertussiscAMP intoxicationlung colonizationlung inflammationpore-forming activityRTX toxinvirulence
UR  - https://www.mdpi.com/1422-0067/22/21/11655
N2  - The whooping cough agent, Bordetella pertussis, secretes an adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) that catalyzes the conversion of intracellular ATP to cAMP and through its signaling annihilates the bactericidal activities of host sentinel phagocytes. In parallel, CyaA permeabilizes host cells by the formation of cation-selective membrane pores that account for the hemolytic activity of CyaA. The pore-forming activity contributes to the overall cytotoxic effect of CyaA in vitro, and it has previously been proposed to synergize with the cAMP-elevating activity in conferring full virulence on B. pertussis in the mouse model of pneumonic infection. CyaA primarily targets myeloid phagocytes through binding of their complement receptor 3 (CR3, integrin alpha(M)beta(2), or CD11b/CD18). However, with a reduced efficacy, the toxin can promiscuously penetrate and permeabilize the cell membrane of a variety of non-myeloid cells that lack CR3 on the cell surface, including airway epithelial cells or erythrocytes, and detectably intoxicates them by cAMP. Here, we used CyaA variants with strongly and selectively enhanced or reduced pore-forming activity that, at the same time, exhibited a full capacity to elevate cAMP concentrations in both CR3-expressing and CR3-non-expressing target cells. Using B. pertussis mutants secreting such CyaA variants, we show that a selective enhancement of the cell-permeabilizing activity of CyaA does not increase the overall virulence and lethality of pneumonic B. pertussis infection of mice any further. In turn, a reduction of the cell-permeabilizing activity of CyaA did not reduce B. pertussis virulence any importantly. These results suggest that the phagocyte-paralyzing cAMP-elevating capacity of CyaA prevails over the cell-permeabilizing activity of CyaA that appears to play an auxiliary role in the biological activity of the CyaA toxin in the course of B. pertussis infections in vivo.
ER  -

HOLUBOVA, J., A. JUHASZ, J. MASIN, O. STANEK, D. JURNECKA, A. OSICKOVA, P. SEBO a R. OSICKA. Selective Enhancement of the Cell-Permeabilizing Activity of Adenylate Cyclase Toxin Does Not Increase Virulence of Bordetella pertussis. \textit{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}. 2021, roč.~22, č.~21, s.~11655-11672. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms222111655.

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