No Correlation between Biofilm-Forming Capacity and Antibiotic Resistance in Environmental Staphylococcus spp.: In Vitro Results

DONADU, Matthew Gavino, Marco FERRARI, Vittorio MAZZARELLO, Stefania ZANETTI, Ivan KUSHKEVYCH, Simon K.-M. R. RITTMANN, Anette STÁJER, Zoltán BARÁTH, Dóra SZABÓ, Edit URBÁN a Márió GAJDÁCS. No Correlation between Biofilm-Forming Capacity and Antibiotic Resistance in Environmental Staphylococcus spp.: In Vitro Results. Pathogens. MDPI, 2022, roč. 11, č. 4, s. 1-14. ISSN 2076-0817. Dostupné z: https://dx.doi.org/10.3390/pathogens11040471.

Základní údaje

• Originální název: No Correlation between Biofilm-Forming Capacity and Antibiotic Resistance in Environmental Staphylococcus spp.: In Vitro Results
• Autoři: DONADU, Matthew Gavino, Marco FERRARI, Vittorio MAZZARELLO, Stefania ZANETTI, Ivan KUSHKEVYCH (203 Česká republika, domácí), Simon K.-M. R. RITTMANN, Anette STÁJER, Zoltán BARÁTH, Dóra SZABÓ, Edit URBÁN a Márió GAJDÁCS.
• Vydání: Pathogens, MDPI, 2022, 2076-0817.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 10606 Microbiology
• Stát vydavatele: Švýcarsko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Impakt faktor: Impact factor: 3.700
• Kód RIV: RIV/00216224:14310/22:00125702
• Organizační jednotka: Přírodovědecká fakulta
• Doi: http://dx.doi.org/10.3390/pathogens11040471
• UT WoS: 000785317100001
• Klíčová slova anglicky: Congo red agar; crystal violet; microtiter plate assay; biofilm formation; Staphylococcus aureus; non-aureus staphylococci; methicillin resistance; multidrug resistance; MDR; phenotypic assay
• Štítky: rivok
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

The production of biofilms is a critical factor in facilitating the survival of Staphylococcus spp. in vivo and in protecting against various environmental noxa. The possible relationship between the antibiotic-resistant phenotype and biofilm-forming capacity has raised considerable interest. The purpose of the study was to assess the interdependence between biofilm-forming capacity and the antibiotic-resistant phenotype in 299 Staphylococcus spp. (S. aureus n = 143, non-aureus staphylococci [NAS] n = 156) of environmental origin. Antimicrobial susceptibility testing and detection of methicillin resistance (MR) was performed. The capacity of isolates to produce biofilms was assessed using Congo red agar (CRA) plates and a crystal violet microtiter-plate-based (CV-MTP) method. MR was identified in 46.9% of S. aureus and 53.8% of NAS isolates (p > 0.05), with resistance to most commonly used drugs being significantly higher in MR isolates compared to methicillin-susceptible isolates. Resistance rates were highest for clindamycin (57.9%), erythromycin (52.2%) and trimethoprim-sulfamethoxazole (51.1%), while susceptibility was retained for most last-resort drugs. Based on the CRA plates, biofilm was produced by 30.8% of S. aureus and 44.9% of NAS (p = 0.014), while based on the CV-MTP method, 51.7% of S. aureus and 62.8% of NAS were identified as strong biofilm producers, respectively (mean OD570 values: S. aureus: 0.779±0.471 vs. NAS: 1.053±0.551; p < 0.001). No significant differences in biofilm formation were observed based on MR (susceptible: 0.824 ± 0.325 vs. resistant: 0.896 ± 0.367; p = 0.101). However, pronounced differences in biofilm formation were identified based on rifampicin susceptibility (S: 0.784 ± 0.281 vs. R: 1.239 ± 0.286; p = 0.011). The mechanistic understanding of the mechanisms Staphylococcus spp. use to withstand harsh environmental and in vivo conditions is crucial to appropriately address the therapy and eradication of these pathogens.