Global Transcriptomic Analysis of Bacteriophage-Host
Interactions between a Kayvirus Therapeutic Phage and
Staphylococcus aureus

J 2022

Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus

FINSTRLOVÁ, Adéla; Ivana MAŠLAŇOVÁ; Bob G. BLASDEL REUTER; Jiří DOŠKAŘ; Friedrich GÖTZ et al.

Základní údaje

Originální název

Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus

Autoři

FINSTRLOVÁ, Adéla; Ivana MAŠLAŇOVÁ; Bob G. BLASDEL REUTER; Jiří DOŠKAŘ; Friedrich GÖTZ a Roman PANTŮČEK

Vydání

Microbiology Spectrum, Washington, DC, American Society for Microbiology, 2022, 2165-0497

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00125731

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

phage-host interactions; Staphylococcus phages; Kayvirus; RNA-Seq; viral transcription; noncoding RNA; prophages; bacteriophage therapy; Staphylococcus aureus; transcriptome

Štítky

14314010, CF GEN, NIVB_PřF, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2025 13:50, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Kayviruses are polyvalent broad host range staphylococcal phages with a potential to combat staphylococcal infections. However, the implementation of rational phage therapy in medicine requires a thorough understanding of the interactions between bacteriophages and pathogens at omics level. To evaluate the effect of a phage used in therapy on its host bacterium, we performed differential transcriptomic analysis by RNA-Seq from bacteriophage K of genus Kayvirus infecting two Staphylococcus aureus strains, prophage-less strain SH1000 and quadruple lysogenic strain Newman. The temporal transcriptional profile of phage K was comparable in both strains except for a few loci encoding hypothetical proteins. Stranded sequencing revealed transcription of phage noncoding RNAs that may play a role in the regulation of phage and host gene expression. The transcriptional response of S. aureus to phage K infection resembles a general stress response with differential expression of genes involved in a DNA damage response. The host transcriptional changes involved upregulation of nucleotide, amino acid and energy synthesis and transporter genes and downregulation of host transcription factors. The interaction of phage K with variable genetic elements of the host showed slight upregulation of gene expression of prophage integrases and antirepressors. The virulence genes involved in adhesion and immune evasion were only marginally affected, making phage K suitable for therapy.

Návaznosti

GA18-13064S, projekt VaV

Název: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

Investor: Grantová agentura ČR, Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

MUNI/A/1325/2021, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10 (Akronym: MBG10)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10

90132, velká výzkumná infrastruktura

Název: NCMG II

Přiložené soubory

spectrum_znptqvbv.00123-22.pdf

Citovat

FINSTRLOVÁ, Adéla; Ivana MAŠLAŇOVÁ; Bob G. BLASDEL REUTER; Jiří DOŠKAŘ; Friedrich GÖTZ a Roman PANTŮČEK. Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus. Microbiology Spectrum. Washington, DC: American Society for Microbiology, 2022, roč. 10, č. 3, s. 1-13, "e00123-22", 13 s. ISSN 2165-0497. Dostupné z: https://doi.org/10.1128/spectrum.00123-22.

@article{1849745,
   author = {Finstrlová, Adéla and Mašlaňová, Ivana and Blasdel Reuter, Bob G. and Doškař, Jiří and Götz, Friedrich and Pantůček, Roman},
   article_location = {Washington, DC},
   article_number = {3},
   doi = {https://doi.org/10.1128/spectrum.00123-22},
   keywords = {phage-host interactions; Staphylococcus phages; Kayvirus; RNA-Seq; viral transcription; noncoding RNA; prophages; bacteriophage therapy; Staphylococcus aureus; transcriptome},
   language = {eng},
   issn = {2165-0497},
   journal = {Microbiology Spectrum},
   title = {Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus},
   url = {https://journals.asm.org/doi/10.1128/spectrum.00123-22},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1849745
AU  - Finstrlová, Adéla - Mašlaňová, Ivana - Blasdel Reuter, Bob G. - Doškař, Jiří - Götz, Friedrich - Pantůček, Roman
PY  - 2022
TI  - Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus
JF  - Microbiology Spectrum
VL  - 10
IS  - 3
SP  - 1-13, "e00123-22"
EP  - 1-13, "e00123-22"
PB  - American Society for Microbiology
SN  - 21650497
KW  - phage-host interactions
KW  - Staphylococcus phages
KW  - Kayvirus
KW  - RNA-Seq
KW  - viral transcription
KW  - noncoding RNA
KW  - prophages
KW  - bacteriophage therapy
KW  - Staphylococcus aureus
KW  - transcriptome
UR  - https://journals.asm.org/doi/10.1128/spectrum.00123-22
N2  - Kayviruses are polyvalent broad host range staphylococcal phages with a potential to combat staphylococcal infections. However, the implementation of rational phage therapy in medicine requires a thorough understanding of the interactions between bacteriophages and pathogens at omics level. To evaluate the effect of a phage used in therapy on its host bacterium, we performed differential transcriptomic analysis by RNA-Seq from bacteriophage K of genus Kayvirus infecting two Staphylococcus aureus strains, prophage-less strain SH1000 and quadruple lysogenic strain Newman. The temporal transcriptional profile of phage K was comparable in both strains except for a few loci encoding hypothetical proteins. Stranded sequencing revealed transcription of phage noncoding RNAs that may play a role in the regulation of phage and host gene expression. The transcriptional response of S. aureus to phage K infection resembles a general stress response with differential expression of genes involved in a DNA damage response. The host transcriptional changes involved upregulation of nucleotide, amino acid and energy synthesis and transporter genes and downregulation of host transcription factors. The interaction of phage K with variable genetic elements of the host showed slight upregulation of gene expression of prophage integrases and antirepressors. The virulence genes involved in adhesion and immune evasion were only marginally affected, making phage K suitable for therapy.
ER  -

FINSTRLOVÁ, Adéla; Ivana MAŠLAŇOVÁ; Bob G. BLASDEL REUTER; Jiří DOŠKAŘ; Friedrich GÖTZ a Roman PANTŮČEK. Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus. \textit{Microbiology Spectrum}. Washington, DC: American Society for Microbiology, 2022, roč.~10, č.~3, s.~1-13, ''e00123-22'', 13 s. ISSN~2165-0497. Dostupné z: https://doi.org/10.1128/spectrum.00123-22.

Přehled o publikaci