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@article{1851997, author = {Švec, Pavel and Králová, Stanislava and Staňková, Eva and Holochová, Pavla and Sedlář, Karel and Koudelková, Sylva and Krsek, Daniel and Grzesiak, Jakub and Šedo, Ondrej and Váczi, Peter and Sah, Urvashi and Gupta, Vipin and Sood, Utkarsh and Lal, Rup and Korpole, Suresh and Sedláček, Ivo}, article_number = {4}, doi = {http://dx.doi.org/10.1099/ijsem.0.005309}, keywords = {Pedobacter fastidiosus sp. nov.; proglacial stream; glacier; Antarctica; taxonomy}, language = {eng}, issn = {1466-5026}, journal = {International Journal of Systematic and Evolutionary Microbiology}, title = {Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica}, url = {https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005309}, volume = {72}, year = {2022} }
TY - JOUR ID - 1851997 AU - Švec, Pavel - Králová, Stanislava - Staňková, Eva - Holochová, Pavla - Sedlář, Karel - Koudelková, Sylva - Krsek, Daniel - Grzesiak, Jakub - Šedo, Ondrej - Váczi, Peter - Sah, Urvashi - Gupta, Vipin - Sood, Utkarsh - Lal, Rup - Korpole, Suresh - Sedláček, Ivo PY - 2022 TI - Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica JF - International Journal of Systematic and Evolutionary Microbiology VL - 72 IS - 4 SP - 1-9 EP - 1-9 PB - Microbiology Society SN - 14665026 KW - Pedobacter fastidiosus sp. nov. KW - proglacial stream KW - glacier KW - Antarctica KW - taxonomy UR - https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005309 N2 - Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA–DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T). ER -
ŠVEC, Pavel, Stanislava KRÁLOVÁ, Eva STAŇKOVÁ, Pavla HOLOCHOVÁ, Karel SEDLÁŘ, Sylva KOUDELKOVÁ, Daniel KRSEK, Jakub GRZESIAK, Ondrej ŠEDO, Peter VÁCZI, Urvashi SAH, Vipin GUPTA, Utkarsh SOOD, Rup LAL, Suresh KORPOLE a Ivo SEDLÁČEK. Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica. \textit{International Journal of Systematic and Evolutionary Microbiology}. Microbiology Society, 2022, roč.~72, č.~4, s.~1-9. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.005309.
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