"Bone Development'' Is an Ontology Group Upregulated in Porcine Oocytes Before In Vitro Maturation: A Microarray Approach

BUDNA, Joanna, Artur BRYJA, Piotr CELICHOWSKI, Wiesława KRANC, Sylwia CIESIÓŁKA, Sylwia BORYS, Marta RYBSKA, Agata KOLECKA-BEDNARCZYK, Michal JEŠETA, Dorota BUKOWSKA, Paweł ANTOSIK, Klaus P BRÜSSOW, Małgorzata BRUSKA, Michał NOWICKI, Maciej ZABEL a Bartosz KEMPISTY. "Bone Development'' Is an Ontology Group Upregulated in Porcine Oocytes Before In Vitro Maturation: A Microarray Approach. DNA and Cell Biology. New York: Mary Ann Liebert Inc., 2017, roč. 36, č. 8, s. 638-646. ISSN 1044-5498. Dostupné z: https://dx.doi.org/10.1089/dna.2017.3677.

Základní údaje

• Originální název: "Bone Development'' Is an Ontology Group Upregulated in Porcine Oocytes Before In Vitro Maturation: A Microarray Approach
• Autoři: BUDNA, Joanna, Artur BRYJA, Piotr CELICHOWSKI, Wiesława KRANC, Sylwia CIESIÓŁKA, Sylwia BORYS, Marta RYBSKA, Agata KOLECKA-BEDNARCZYK, Michal JEŠETA (203 Česká republika, domácí), Dorota BUKOWSKA, Paweł ANTOSIK, Klaus P BRÜSSOW, Małgorzata BRUSKA, Michał NOWICKI, Maciej ZABEL a Bartosz KEMPISTY (garant).
• Vydání: DNA and Cell Biology, New York, Mary Ann Liebert Inc. 2017, 1044-5498.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30214 Obstetrics and gynaecology
• Stát vydavatele: Spojené státy
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Impakt faktor: Impact factor: 2.634
• Kód RIV: RIV/00216224:14110/17:00124599
• Organizační jednotka: Lékařská fakulta
• Doi: http://dx.doi.org/10.1089/dna.2017.3677
• UT WoS: 000407208600004
• Klíčová slova anglicky: pig; oocytes; microarray; in vitro maturation
• Štítky: rivok
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Mammalian cumulus-oocyte complexes (COCs) reach full developmental capability during folliculogenesis and oogenesis. It is well recognized that only gametes achieving MII stage after in vivo or in vitro maturation (IVM) are successfully fertilized by a single spermatozoon. Although the process of oocyte nuclear and/ or cytoplasmic maturation in pigs is well determined, there exist many differences that promote these processes in vivo and in vitro. Therefore, this study aimed to investigate the differences in RNA expression profiles between porcine oocytes before and after IVM using microarray and real-time quantitative polymerase chain reaction (RT-qPCR) assays. Experiments were performed on oocytes isolated from 55 pubertal crossbred Landrace gilts. The oocytes were analyzed both before and after IVM and only Brilliant Cresyl Blue (BCB)positive gametes were used for subsequent microarray analysis (Affymetrix) and RT-qPCR analysis. The microarray assay, which measures expression of 12,258 transcripts, revealed 419 differentially expressed transcripts in porcine oocytes, from which 379 were downregulated and 40 were upregulated before IVM compared to those analyzed after IVM. After DAVID analysis, we found eight different transcripts, including IHH, BMP1, WWTR1, CHRDL1, KLF10, EIF2AK3, MMP14, and STC1. Their expression is related to the "bone development'' ontology group and was further subjected to hierarchical clusterization. Using RT-qPCR analysis, we confirmed the results of the microarray assay, showing increased expression of the eight genes in oocytes before IVM compared to oocytes after maturation in vitro. It has been suggested that "bone development'' belongs to one ontological group involving genes substantially upregulated in porcine oocytes before IVM. We suggest that the gamete mRNA expression profile before IVM may comprise stored transcripts, which are templates for protein biosynthesis following fertilization. We also hypothesize that these mRNAs may be a specific "fingerprint'' of folliculogenesis and oogenesis in pigs.