What Digital Immunoassays Can Learn from Ambient Analyte
Theory: A Perspective

J 2022

What Digital Immunoassays Can Learn from Ambient Analyte Theory: A Perspective

GORRIS, Hans-Heiner a Tero SOUKKA

Základní údaje

Originální název

What Digital Immunoassays Can Learn from Ambient Analyte Theory: A Perspective

Autoři

GORRIS, Hans-Heiner a Tero SOUKKA

Vydání

Analytical chemistry, American Chemical Society, 2022, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00125870

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Assays; Biopolymers; Immunoassays; Immunology; Peptides and proteins

Štítky

14313050, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 5. 2022 11:09, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Immunoassays are important tools for clinical diagnosis as well as environmental and food analysis because they enable highly sensitive and quantitative measurements of analyte concentrations. In the 1980s, Roger Ekins suggested to improve the sensitivity of immunoassays by employing microspot assays, which are carried out under ambient analyte conditions and do not change the bulk analyte concentration of a sample during a measurement. More recently, the measurement of single analyte molecules has additionally attracted wide research interest. Although the ability to detect a single analyte molecule is not synonymous with the highest analytical sensitivity, single-molecule detection makes new routes accessible to avoiding background noise. This perspective follows the development of solid-phase immunoassays from the design of label techniques to single-molecule (digital) assays against the backdrop of Ekins’s fundamental work on immunoassay theory. The essential aspects of both ambient analyte and digital assay approaches are presented as a guideline to finding a balance between the speed, sensitivity, and precision of immunoassays.

Citovat

GORRIS, Hans-Heiner a Tero SOUKKA. What Digital Immunoassays Can Learn from Ambient Analyte Theory: A Perspective. Analytical chemistry. American Chemical Society, 2022, roč. 94, č. 16, s. 6073-6083. ISSN 0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.1c05591.

@article{1855526,
   author = {Gorris, HansandHeiner and Soukka, Tero},
   article_number = {16},
   doi = {https://doi.org/10.1021/acs.analchem.1c05591},
   keywords = {Assays; Biopolymers; Immunoassays; Immunology; Peptides and proteins},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical chemistry},
   title = {What Digital Immunoassays Can Learn from Ambient Analyte Theory: A Perspective},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.1c05591},
   volume = {94},
   year = {2022}
}

TY  - JOUR
ID  - 1855526
AU  - Gorris, Hans-Heiner - Soukka, Tero
PY  - 2022
TI  - What Digital Immunoassays Can Learn from Ambient Analyte Theory: A Perspective
JF  - Analytical chemistry
VL  - 94
IS  - 16
SP  - 6073-6083
EP  - 6073-6083
PB  - American Chemical Society
SN  - 00032700
KW  - Assays
KW  - Biopolymers
KW  - Immunoassays
KW  - Immunology
KW  - Peptides and proteins
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.1c05591
N2  - Immunoassays are important tools for clinical diagnosis as well as environmental and food analysis because they enable highly sensitive and quantitative measurements of analyte concentrations. In the 1980s, Roger Ekins suggested to improve the sensitivity of immunoassays by employing microspot assays, which are carried out under ambient analyte conditions and do not change the bulk analyte concentration of a sample during a measurement. More recently, the measurement of single analyte molecules has additionally attracted wide research interest. Although the ability to detect a single analyte molecule is not synonymous with the highest analytical sensitivity, single-molecule detection makes new routes accessible to avoiding background noise. This perspective follows the development of solid-phase immunoassays from the design of label techniques to single-molecule (digital) assays against the backdrop of Ekins’s fundamental work on immunoassay theory. The essential aspects of both ambient analyte and digital assay approaches are presented as a guideline to finding a balance between the speed, sensitivity, and precision of immunoassays.
ER  -

GORRIS, Hans-Heiner a Tero SOUKKA. What Digital Immunoassays Can Learn from Ambient Analyte Theory: A Perspective. \textit{Analytical chemistry}. American Chemical Society, 2022, roč.~94, č.~16, s.~6073-6083. ISSN~0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.1c05591.

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