Revisiting boundaries of ancient DNA study

a 2022

Revisiting boundaries of ancient DNA study

NOVOTNÁ, Kateřina; Eva DROZDOVÁ; Dana FIALOVÁ; Eva CHOCHOLOVÁ; Kristýna BRZOBOHATÁ et al.

Základní údaje

Originální název

Revisiting boundaries of ancient DNA study

Autoři

NOVOTNÁ, Kateřina; Eva DROZDOVÁ; Dana FIALOVÁ; Eva CHOCHOLOVÁ a Kristýna BRZOBOHATÁ

Vydání

The Biomania Student Scientific Meeting 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00125913

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0040-0

Klíčová slova česky

starobylá DNA; Velkomoravská říše; chemická konzervace; zubní kámen

Klíčová slova anglicky

ancient DNA; Great Moravian Empire; chemical treatment; dental calculus

Změněno: 1. 6. 2022 09:29, Mgr. Kateřina Novotná

Anotace

V originále

Genetical studies of ancient populations have a lot of limitations and challenges ranging from the degradation of DNA over time to rarely optimal conditions for the preservation of remains both before and after excavation. A lot of anthropologists and archaeologists tried to preserve as much skeletal material as possible via treatment of chemical agents, which is still used to this day in museum collections. Aside from this approach posing danger to the scientist, the chemical treatment was believed to interact with DNA and thus excluding such treated remains from the genetical and molecular analyses. Our study has shown that we are able to gain information from chemically treated samples and that it’s even possible to analyze DNA from them. This would make research on treated remains from museum archives possible as well, of which medical fields could also benefit. In our research, we have focused on the archaeological site Pohansko – Kostel dated to the early Middle ages, times of the Great Moravian Empire. This site has been excavated in the 50s and 60s and at this time it was common to treat skeletal remains with celluloid dissolved in amyl acetate for better preservation. In our study, we have managed to isolate and sequence aDNA from the dental calculus of said samples thus proving similar kinds of studies possible.

Návaznosti

MUNI/A/1325/2021, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10 (Akronym: MBG10)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10

MUNI/C/1703/2018, interní kód MU

Název: Analýza historického zubního kamene ošetřeného chemickou konzervací

Investor: Masarykova univerzita, Analýza historického zubního kamene ošetřeného chemickou konzervací, DO R. 2020 - Program rektora

Citovat

NOVOTNÁ, Kateřina; Eva DROZDOVÁ; Dana FIALOVÁ; Eva CHOCHOLOVÁ a Kristýna BRZOBOHATÁ. Revisiting boundaries of ancient DNA study. In The Biomania Student Scientific Meeting 2022. 2022. ISBN 978-80-280-0040-0.

@proceedings{1857422,
   author = {Novotná, Kateřina and Drozdová, Eva and Fialová, Dana and Chocholová, Eva and Brzobohatá, Kristýna},
   booktitle = {The Biomania Student Scientific Meeting 2022},
   keywords = {ancient DNA; Great Moravian Empire; chemical treatment; dental calculus},
   language = {eng},
   isbn = {978-80-280-0040-0},
   title = {Revisiting boundaries of ancient DNA study},
   url = {http://www.biomania.cz/udalosti-a-akce/biomania-student-scientific-meeting-2022/},
   year = {2022}
}

TY  - CONF
ID  - 1857422
AU  - Novotná, Kateřina - Drozdová, Eva - Fialová, Dana - Chocholová, Eva - Brzobohatá, Kristýna
PY  - 2022
TI  - Revisiting boundaries of ancient DNA study
SN  - 9788028000400
KW  - ancient DNA
KW  - Great Moravian Empire
KW  - chemical treatment
KW  - dental calculus
UR  - http://www.biomania.cz/udalosti-a-akce/biomania-student-scientific-meeting-2022/
N2  - Genetical studies of ancient populations have a lot of limitations and challenges ranging from the degradation of DNA over time to rarely optimal conditions for the preservation of remains both before and after excavation. A lot of anthropologists and archaeologists tried to preserve as much skeletal material as possible via treatment of chemical agents, which is still used to this day in museum collections. Aside from this approach posing danger to the scientist, the chemical treatment was believed to interact with DNA and thus excluding such treated remains from the genetical and molecular analyses. Our study has shown that we are able to gain information from chemically treated samples and that it’s even possible to analyze DNA from them. This would make research on treated remains from museum archives possible as well, of which medical fields could also benefit. In our research, we have focused on the archaeological site Pohansko – Kostel dated to the early Middle ages, times of the Great Moravian Empire. This site has been excavated in the 50s and 60s and at this time it was common to treat skeletal remains with celluloid dissolved in amyl acetate for better preservation. In our study, we have managed to isolate and sequence aDNA from the dental calculus of said samples thus proving similar kinds of studies possible.
ER  -

NOVOTNÁ, Kateřina; Eva DROZDOVÁ; Dana FIALOVÁ; Eva CHOCHOLOVÁ a Kristýna BRZOBOHATÁ. Revisiting boundaries of ancient DNA study. In \textit{The Biomania Student Scientific Meeting 2022}. 2022. ISBN~978-80-280-0040-0.

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