Characterizing toxicity pathways of fluoxetine to predict
adverse outcomes in adult fathead minnows (Pimephales promelas)

J 2022

Characterizing toxicity pathways of fluoxetine to predict adverse outcomes in adult fathead minnows (Pimephales promelas)

COLVILLE, Carly; Alper James ALCARAZ; Derek GREEN; Bradley PARK; Jianguo XIA et. al.

Základní údaje

Originální název

Characterizing toxicity pathways of fluoxetine to predict adverse outcomes in adult fathead minnows (Pimephales promelas)

Autoři

COLVILLE, Carly; Alper James ALCARAZ; Derek GREEN; Bradley PARK; Jianguo XIA; Othman SOUFAN; Pavel HRUŠKA; David POTĚŠIL; Zbyněk ZDRÁHAL; Doug CRUMP; Niladri BASU; Natacha HOGAN a Markus HECKER

Vydání

SCIENCE OF THE TOTAL ENVIRONMENT, NETHERLANDS, ELSEVIER, 2022, 0048-9697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 9.800

Kód RIV

RIV/00216224:14310/22:00125949

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.scitotenv.2021.152747

UT WoS

000766818100009

EID Scopus

2-s2.0-85122801092

Klíčová slova anglicky

Fathead minnow Fluoxetine Transcriptomics Proteomics Fecundity Toxicity pathways

Štítky

143160, 143180, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:59, Ing. Martina Blahová

Anotace

V originále

Current ecotoxicity testing programs are impeded as they predominantly rely on slow and expensive animal tests measuring adverse outcomes. Therefore, new approach methodologies (NAMs) increasingly involve short-term mechanistic assays that employ molecular endpoints to predict adverse outcomes of regulatory relevance. This study aimed to elucidate the application of NAMs in adult fathead minnows using fluoxetine (FLX) as a model compound. Fish were exposed to three FLX concentrations (measured: 2.42, 10.7, and 56.7 mu gL(-1)) and a control. After 96 h, molecular toxicity signatures were characterized using proteomics and transcriptomics analyses in livers and brains of a sub-set of fish. The remaining fish were sampled at 21 days and assessed for liver histopathology and morphometric measurements. Fecundity was monitored throughout the study. In the livers, 56.7 mu gL(-1) FLX caused enrichment of PPAR signaling in the proteome and fatty acid-related pathways in the transcriptome, potential upstream responses that led to lipid-type vacuolation of hepatocytes, observed via histopathology. Upregulated genes in the brain suggested alterations in serotonin-related signaling processes and reproductive behaviour, which may explain the observed signifi-cant decrease in fecundity. While the relationships between molecular responses and adverse outcomes remain complex, this research provided important insights into the mechanistic toxicity of FLX.

Návaznosti

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

COLVILLE, Carly; Alper James ALCARAZ; Derek GREEN; Bradley PARK; Jianguo XIA; Othman SOUFAN; Pavel HRUŠKA; David POTĚŠIL; Zbyněk ZDRÁHAL; Doug CRUMP; Niladri BASU; Natacha HOGAN a Markus HECKER. Characterizing toxicity pathways of fluoxetine to predict adverse outcomes in adult fathead minnows (Pimephales promelas). SCIENCE OF THE TOTAL ENVIRONMENT. NETHERLANDS: ELSEVIER, 2022, roč. 817, APR, s. "152747", 10 s. ISSN 0048-9697. Dostupné z: https://doi.org/10.1016/j.scitotenv.2021.152747.

@article{1858732,
   author = {Colville, Carly and Alcaraz, Alper James and Green, Derek and Park, Bradley and Xia, Jianguo and Soufan, Othman and Hruška, Pavel and Potěšil, David and Zdráhal, Zbyněk and Crump, Doug and Basu, Niladri and Hogan, Natacha and Hecker, Markus},
   article_location = {NETHERLANDS},
   article_number = {APR},
   doi = {https://doi.org/10.1016/j.scitotenv.2021.152747},
   keywords = {Fathead minnow Fluoxetine Transcriptomics Proteomics Fecundity Toxicity pathways},
   language = {eng},
   issn = {0048-9697},
   journal = {SCIENCE OF THE TOTAL ENVIRONMENT},
   title = {Characterizing toxicity pathways of fluoxetine to predict adverse outcomes in adult fathead minnows (Pimephales promelas)},
   url = {http://www.sciencedirect.com/science/article/pii/S0048969721078268},
   volume = {817},
   year = {2022}
}

TY  - JOUR
ID  - 1858732
AU  - Colville, Carly - Alcaraz, Alper James - Green, Derek - Park, Bradley - Xia, Jianguo - Soufan, Othman - Hruška, Pavel - Potěšil, David - Zdráhal, Zbyněk - Crump, Doug - Basu, Niladri - Hogan, Natacha - Hecker, Markus
PY  - 2022
TI  - Characterizing toxicity pathways of fluoxetine to predict adverse outcomes in adult fathead minnows (Pimephales promelas)
JF  - SCIENCE OF THE TOTAL ENVIRONMENT
VL  - 817
IS  - APR
SP  - "152747"
EP  - "152747"
PB  - ELSEVIER
SN  - 00489697
KW  - Fathead minnow Fluoxetine Transcriptomics Proteomics Fecundity Toxicity pathways
UR  - http://www.sciencedirect.com/science/article/pii/S0048969721078268
N2  - Current ecotoxicity testing programs are impeded as they predominantly rely on slow and expensive animal tests measuring adverse outcomes. Therefore, new approach methodologies (NAMs) increasingly involve short-term mechanistic assays that employ molecular endpoints to predict adverse outcomes of regulatory relevance. This study aimed to elucidate the application of NAMs in adult fathead minnows using fluoxetine (FLX) as a model compound. Fish were exposed to three FLX concentrations (measured: 2.42, 10.7, and 56.7 mu gL(-1)) and a control. After 96 h, molecular toxicity signatures were characterized using proteomics and transcriptomics analyses in livers and brains of a sub-set of fish. The remaining fish were sampled at 21 days and assessed for liver histopathology and morphometric measurements. Fecundity was monitored throughout the study. In the livers, 56.7 mu gL(-1) FLX caused enrichment of PPAR signaling in the proteome and fatty acid-related pathways in the transcriptome, potential upstream responses that led to lipid-type vacuolation of hepatocytes, observed via histopathology. Upregulated genes in the brain suggested alterations in serotonin-related signaling processes and reproductive behaviour, which may explain the observed signifi-cant decrease in fecundity. While the relationships between molecular responses and adverse outcomes remain complex, this research provided important insights into the mechanistic toxicity of FLX.
ER  -

COLVILLE, Carly; Alper James ALCARAZ; Derek GREEN; Bradley PARK; Jianguo XIA; Othman SOUFAN; Pavel HRUŠKA; David POTĚŠIL; Zbyněk ZDRÁHAL; Doug CRUMP; Niladri BASU; Natacha HOGAN a Markus HECKER. Characterizing toxicity pathways of fluoxetine to predict adverse outcomes in adult fathead minnows (Pimephales promelas). \textit{SCIENCE OF THE TOTAL ENVIRONMENT}. NETHERLANDS: ELSEVIER, 2022, roč.~817, APR, s.~''152747'', 10 s. ISSN~0048-9697. Dostupné z: https://doi.org/10.1016/j.scitotenv.2021.152747.

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