Expandable Lung Epithelium Differentiated from Human Embryonic
Stem Cells

J 2022

Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

KOTASOVÁ, Hana; Michaela CAPANDOVÁ; Vendula PELKOVÁ; Jana DUMKOVÁ; Zuzana SUMBALOVÁ KOLEDOVÁ et. al.

Základní údaje

Originální název

Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

Autoři

Vydání

TISSUE ENGINEERING AND REGENERATIVE MEDICINE, SEOUL, KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC, 2022, 1738-2696

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20602 Medical laboratory technology

Stát vydavatele

Korejská republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.600

Kód RIV

RIV/00216224:14110/22:00126034

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1007/s13770-022-00458-0

UT WoS

000807342200001

EID Scopus

2-s2.0-85131534857

Klíčová slova anglicky

Lung; Epithelium; hESC; Differentiation; Foregut endoderm

Štítky

14110128, 14110517, 14313010, 14314010, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 3. 2023 07:13, Mgr. Tereza Miškechová

Anotace

V originále

Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.

Návaznosti

MUNI/A/1390/2020, interní kód MU

Název: Analytická a fyzikální chemie ve výzkumu biologických, geologických a syntetických materiálů (Akronym: BIOGEOSYNT)

Investor: Masarykova univerzita, Analytická a fyzikální chemie ve výzkumu biologických, geologických a syntetických materiálů

MUNI/A/1398/2021, interní kód MU

Název: Zdroje pro tkáňové inženýrství 12 (Akronym: TissueEng 12)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 12

MUNI/A/1689/2020, interní kód MU

Název: Zdroje pro tkáňové inženýrství 11 (Akronym: TissueEng 11)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 11

NV16-31501A, projekt VaV

Název: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

ROZV/28/LF23/2020, interní kód MU

Název: Objasněni vlivu ztráty TUSC3 na změnu fenotypových vlastnosti buněk ovariálního povrchového epitelu.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Objasněni vlivu ztráty TUSC3 na změnu fenotypových vlastností buněk ovariálního povrchového epitelu., Interní rozvojové projekty

Citovat

KOTASOVÁ, Hana; Michaela CAPANDOVÁ; Vendula PELKOVÁ; Jana DUMKOVÁ; Zuzana SUMBALOVÁ KOLEDOVÁ; Ján REMŠÍK; Karel SOUČEK; Zuzana GARLÍKOVÁ; Veronika SEDLÁKOVÁ; Anas RABATA; Petr VAŇHARA; Lukáš MORÁŇ; Lukáš PEČINKA; Volodymyr POROKH; Martin KUČÍREK; Libor STREIT; Josef HAVEL a Aleš HAMPL. Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells. TISSUE ENGINEERING AND REGENERATIVE MEDICINE. SEOUL: KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC, 2022, roč. 19, č. 5, s. 1033-1050. ISSN 1738-2696. Dostupné z: https://doi.org/10.1007/s13770-022-00458-0.

@article{1860588,
   author = {Kotasová, Hana and Capandová, Michaela and Pelková, Vendula and Dumková, Jana and Sumbalová Koledová, Zuzana and Remšík, Ján and Souček, Karel and Garlíková, Zuzana and Sedláková, Veronika and Rabata, Anas and Vaňhara, Petr and Moráň, Lukáš and Pečinka, Lukáš and Porokh, Volodymyr and Kučírek, Martin and Streit, Libor and Havel, Josef and Hampl, Aleš},
   article_location = {SEOUL},
   article_number = {5},
   doi = {https://doi.org/10.1007/s13770-022-00458-0},
   keywords = {Lung; Epithelium; hESC; Differentiation; Foregut endoderm},
   language = {eng},
   issn = {1738-2696},
   journal = {TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
   title = {Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells},
   url = {https://link.springer.com/article/10.1007/s13770-022-00458-0},
   volume = {19},
   year = {2022}
}

TY  - JOUR
ID  - 1860588
AU  - Kotasová, Hana - Capandová, Michaela - Pelková, Vendula - Dumková, Jana - Sumbalová Koledová, Zuzana - Remšík, Ján - Souček, Karel - Garlíková, Zuzana - Sedláková, Veronika - Rabata, Anas - Vaňhara, Petr - Moráň, Lukáš - Pečinka, Lukáš - Porokh, Volodymyr - Kučírek, Martin - Streit, Libor - Havel, Josef - Hampl, Aleš
PY  - 2022
TI  - Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
JF  - TISSUE ENGINEERING AND REGENERATIVE MEDICINE
VL  - 19
IS  - 5
SP  - 1033-1050
EP  - 1033-1050
PB  - KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
SN  - 17382696
KW  - Lung
KW  - Epithelium
KW  - hESC
KW  - Differentiation
KW  - Foregut endoderm
UR  - https://link.springer.com/article/10.1007/s13770-022-00458-0
N2  - Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.
ER  -

KOTASOVÁ, Hana; Michaela CAPANDOVÁ; Vendula PELKOVÁ; Jana DUMKOVÁ; Zuzana SUMBALOVÁ KOLEDOVÁ; Ján REMŠÍK; Karel SOUČEK; Zuzana GARLÍKOVÁ; Veronika SEDLÁKOVÁ; Anas RABATA; Petr VAŇHARA; Lukáš MORÁŇ; Lukáš PEČINKA; Volodymyr POROKH; Martin KUČÍREK; Libor STREIT; Josef HAVEL a Aleš HAMPL. Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells. \textit{TISSUE ENGINEERING AND REGENERATIVE MEDICINE}. SEOUL: KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC, 2022, roč.~19, č.~5, s.~1033-1050. ISSN~1738-2696. Dostupné z: https://doi.org/10.1007/s13770-022-00458-0.

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