| PÍPAL, Marek, Jiří NOVÁK, Aneta RAFAJOVÁ, Marie SMUTNÁ a Klára HILSCHEROVÁ. Teratogenicity of retinoids detected in surface waters in zebrafish embryos and its predictability by in vitro assays. Aquatic toxicology. AMSTERDAM: ELSEVIER SCIENCE BV, 2022, roč. 246, May 2022, s. 1-11. ISSN 0166-445X. Dostupné z: https://dx.doi.org/10.1016/j.aquatox.2022.106151. |
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@article{1863610,
author = {Pípal, Marek and Novák, Jiří and Rafajová, Aneta and Smutná, Marie and Hilscherová, Klára},
article_location = {AMSTERDAM},
article_number = {May 2022},
doi = {http://dx.doi.org/10.1016/j.aquatox.2022.106151},
keywords = {Retinoids; Teratogenicity; RAR; In vitro; Zebrafish},
language = {eng},
issn = {0166-445X},
journal = {Aquatic toxicology},
title = {Teratogenicity of retinoids detected in surface waters in zebrafish embryos and its predictability by in vitro assays},
url = {https://www.sciencedirect.com/science/article/pii/S0166445X22000789?via%3Dihub},
volume = {246},
year = {2022}
}
TY - JOUR
ID - 1863610
AU - Pípal, Marek - Novák, Jiří - Rafajová, Aneta - Smutná, Marie - Hilscherová, Klára
PY - 2022
TI - Teratogenicity of retinoids detected in surface waters in zebrafish embryos and its predictability by in vitro assays
JF - Aquatic toxicology
VL - 246
IS - May 2022
SP - 1-11
EP - 1-11
PB - ELSEVIER SCIENCE BV
SN - 0166445X
KW - Retinoids
KW - Teratogenicity
KW - RAR
KW - In vitro
KW - Zebrafish
UR - https://www.sciencedirect.com/science/article/pii/S0166445X22000789?via%3Dihub
N2 - Retinoids are newly detected compounds in aquatic ecosystems associated with cyanobacterial water blooms. Their potential health risks are only scarcely described despite numerous detections of all-trans retinoic acid (ATRA) and its derivatives in the environment. Besides the known teratogen ATRA there is only little or no information about their potency and namely their effects in vivo. We characterize ATRA and 8 other retinoids reported to occur in the environment for their bioactivity and teratogenicity using four in vitro reporter gene assays and zebrafish (Danio rerio) embryotoxicity assay. Our results document the ability of these compounds to interfere with retinoid signalling and cause teratogenicity at environmentally relevant levels with EC50 values at nM (hundreds of ng/L) levels and teratogenic indexes ranging from 2.8 (9cis retinoic acid) to 15.8 (retinal). The relative potency of individual compounds for teratogenicity ranged from 0.059 (retinal) to 0.96 (5,6-epoxy ATRA) when compared to ATRA. An environmentally relevant mixture of retinoids was tested showing good predictability of teratogenicity from the in vitro activities and additive toxicity of the mixture. The high teratogenicity of the newly described compounds associated with cyanobacteria presents a concern for developmental stages due to high conservation of the retinoid signalling across vertebrates.
ER -
PÍPAL, Marek, Jiří NOVÁK, Aneta RAFAJOVÁ, Marie SMUTNÁ a Klára HILSCHEROVÁ. Teratogenicity of retinoids detected in surface waters in zebrafish embryos and its predictability by in vitro assays. \textit{Aquatic toxicology}. AMSTERDAM: ELSEVIER SCIENCE BV, 2022, roč.~246, May 2022, s.~1-11. ISSN~0166-445X. Dostupné z: https://dx.doi.org/10.1016/j.aquatox.2022.106151.
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