In vitro testicular toxicity of environmentally relevant
endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal
Leydig TM3 cells

J 2022

In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells

SYCHROVÁ, Eliška; Affiefa YAWER; Petra LABOHÁ; Amrita BASU; Aneta DYDOWICZOVÁ et al.

Základní údaje

Originální název

In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells

Autoři

SYCHROVÁ, Eliška ; Affiefa YAWER; Petra LABOHÁ; Amrita BASU; Aneta DYDOWICZOVÁ; Ishita VIRMANI; Pavel BABICA a Iva SOVADINOVÁ

Vydání

Environmental Toxicology and Pharmacology, Amsterdam, Elsevier Science B.V. 2022, 1382-6689

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00126192

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

3D in vitro models; Endocrine-disrupting chemicals; Leydig cells; Lipotoxicity; Reproductive toxicity; Spheroids

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 6. 2025 10:53, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

The testis is a priority organ for developing alternative models to assess male reproductive health hazards of chemicals. This study characterized a 3D in vitro model of murine prepubertal Leydig TM3 cells with improved expression of steroidogenesis markers suitable for image-based screening of testicular toxicity. This 3D scaffold free spheroid model was applied to explore the impact of prototypical endocrine-disrupting chemicals (EDCs) and environmental reprotoxicants (benzo[a]pyrene, 2-and 9-methylanthracenes, fluoranthene, triclosan, triclocarban, methoxychlor) on male reproductive health. The results were compared to the male reprotoxicity potential of EDCs assessed in a traditional monolayer (2D) culture. The testicular toxicity was dependent not only on the type of culture (2D vs. 3D models) but also on the duration of exposure. Benzo[a]pyrene and triclocarban were the most active compounds, eliciting cytotoxic effects in prepubertal Leydig cells at low micromolar concentrations, which might be a mechanism contributing to their male reprotoxicity.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

GA22-30004S, projekt VaV

Název: Alternativní přístupy v hodnocení mužské reprodukční toxicity chemických směsí bez využití zvířat

Investor: Grantová agentura ČR, Alternativní přístupy v hodnocení mužské reprodukční toxicity chemických směsí bez využití zvířat

GJ16-10775Y, projekt VaV

Název: Mezibuněčná komunikace zprostředkovaná mezerovými spoji jako cíl endokrinních disruptorů v testikulárních buňkách

Investor: Grantová agentura ČR, Mezibuněčná komunikace zprostředkovaná mezerovými spoji jako cíl endokrinních disruptorů v testikulárních buňkách

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Citovat

SYCHROVÁ, Eliška; Affiefa YAWER; Petra LABOHÁ; Amrita BASU; Aneta DYDOWICZOVÁ; Ishita VIRMANI; Pavel BABICA a Iva SOVADINOVÁ. In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells. Environmental Toxicology and Pharmacology. Amsterdam: Elsevier Science B.V., 2022, roč. 93, July 2022, s. 1-16. ISSN 1382-6689. Dostupné z: https://doi.org/10.1016/j.etap.2022.103869.

@article{1863806,
   author = {Sychrová, Eliška and Yawer, Affiefa and Labohá, Petra and Basu, Amrita and Dydowiczová, Aneta and Virmani, Ishita and Babica, Pavel and Sovadinová, Iva},
   article_location = {Amsterdam},
   article_number = {July 2022},
   doi = {https://doi.org/10.1016/j.etap.2022.103869},
   keywords = {3D in vitro models; Endocrine-disrupting chemicals; Leydig cells; Lipotoxicity; Reproductive toxicity; Spheroids},
   language = {eng},
   issn = {1382-6689},
   journal = {Environmental Toxicology and Pharmacology},
   title = {In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells},
   url = {https://www.sciencedirect.com/science/article/pii/S138266892200062X?via%3Dihub},
   volume = {93},
   year = {2022}
}

TY  - JOUR
ID  - 1863806
AU  - Sychrová, Eliška - Yawer, Affiefa - Labohá, Petra - Basu, Amrita - Dydowiczová, Aneta - Virmani, Ishita - Babica, Pavel - Sovadinová, Iva
PY  - 2022
TI  - In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells
JF  - Environmental Toxicology and Pharmacology
VL  - 93
IS  - July 2022
SP  - 1-16
EP  - 1-16
PB  - Elsevier Science B.V.
SN  - 13826689
KW  - 3D in vitro models
KW  - Endocrine-disrupting chemicals
KW  - Leydig cells
KW  - Lipotoxicity
KW  - Reproductive toxicity
KW  - Spheroids
UR  - https://www.sciencedirect.com/science/article/pii/S138266892200062X?via%3Dihub
N2  - The testis is a priority organ for developing alternative models to assess male reproductive health hazards of chemicals. This study characterized a 3D in vitro model of murine prepubertal Leydig TM3 cells with improved expression of steroidogenesis markers suitable for image-based screening of testicular toxicity. This 3D scaffold free spheroid model was applied to explore the impact of prototypical endocrine-disrupting chemicals (EDCs) and environmental reprotoxicants (benzo[a]pyrene, 2-and 9-methylanthracenes, fluoranthene, triclosan, triclocarban, methoxychlor) on male reproductive health. The results were compared to the male reprotoxicity potential of EDCs assessed in a traditional monolayer (2D) culture. The testicular toxicity was dependent not only on the type of culture (2D vs. 3D models) but also on the duration of exposure. Benzo[a]pyrene and triclocarban were the most active compounds, eliciting cytotoxic effects in prepubertal Leydig cells at low micromolar concentrations, which might be a mechanism contributing to their male reprotoxicity.
ER  -

SYCHROVÁ, Eliška; Affiefa YAWER; Petra LABOHÁ; Amrita BASU; Aneta DYDOWICZOVÁ; Ishita VIRMANI; Pavel BABICA a Iva SOVADINOVÁ. In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells. \textit{Environmental Toxicology and Pharmacology}. Amsterdam: Elsevier Science B.V., 2022, roč.~93, July 2022, s.~1-16. ISSN~1382-6689. Dostupné z: https://doi.org/10.1016/j.etap.2022.103869.

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