Systematic position of the Clicking Frog (Kassinula Laurent,
1940), the problem of chimeric sequences and the revised
classification of the family Hyperoliidae

J 2022

Systematic position of the Clicking Frog (Kassinula Laurent, 1940), the problem of chimeric sequences and the revised classification of the family Hyperoliidae

NEČAS, Tadeáš; Jos KIELGAST; Zoltán T. NAGY; Zacharie Kusamba CHIFUNDERA; Václav GVOŽDÍK et al.

Základní údaje

Originální název

Systematic position of the Clicking Frog (Kassinula Laurent, 1940), the problem of chimeric sequences and the revised classification of the family Hyperoliidae

Autoři

NEČAS, Tadeáš; Jos KIELGAST; Zoltán T. NAGY; Zacharie Kusamba CHIFUNDERA a Václav GVOŽDÍK

Vydání

Molecular Phylogenetics and Evolution, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2022, 1055-7903

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10602 Biology , Evolutionary biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00126211

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Afrotropics; Amphibians; Congo Basin; Suprageneric classification; Systematics

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 12. 2022 13:51, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The systematics of the African frog family Hyperoliidae has undergone turbulent changes in last decades. Representatives of several genera have not been genetically investigated or with only limited data, and their phylogenetic positions are thus still not reliably known. This is the case of the De Witte's Clicking Frog (Kassinula wittei) which belongs to a monotypic genus. This miniature frog occurs in a poorly studied region, southeastern Democratic Republic of the Congo, northern Zambia, Angola. So far it is not settled whether this genus belongs to the subfamily Kassininae as a relative of the genus Kassina, or to the subfamily Hyperoliinae as a relative of the genus Afrixalus. Here we present for the first time a multilocus phylogenetic reconstruction (using five nuclear and one mitochondrial marker) of the family Hyperoliidae, including Kassinula. We demonstrate with high confidence that Kassinula is a member of Hyperoliinae belonging to a clade also containing Afrixalus (subSaharan Africa), Heterixalus (Madagascar) and Tachycnemis (Seychelles). We find that Kassinula represents a divergent lineage (17-25 Mya), which supports its separate genus-level status, but its exact systematic position remains uncertain. We propose to name the clade to which the above four genera belong as the tribe Tachycnemini Channing, 1989. A new taxonomy of the family Hyperoliidae was recently proposed by Dubois et al. (2021: Megataxa 5, 1-738). We demonstrate here that the new taxonomy was based on a partially erroneous phylogenetic reconstruction resulting from a supermatrix analysis of chimeric DNA sequences combining data from two families, Hyperoliidae and Arthroleptidae (the case of Cryptothylax). We therefore correct the erroneous part and propose a new, revised suprageneric taxonomy of the family Hyperoliidae. We also emphasize the importance of inspecting individual genetic markers before their concatenation or coalescent-based tree reconstructions to avoid analyses of chimeric DNA sequences producing incorrect phylogenetic reconstructions. Especially when phylogenetic reconstructions are used to propose taxonomies and systematic classifications.

Návaznosti

MUNI/A/1401/2021, interní kód MU

Název: Ekologické a Evoluční Mechanismy v utváření současných Ekosystémů (Akronym: EEMOP)

Investor: Masarykova univerzita, Ekologické a Evoluční Mechanismy v utváření současných Ekosystémů

Citovat

NEČAS, Tadeáš; Jos KIELGAST; Zoltán T. NAGY; Zacharie Kusamba CHIFUNDERA a Václav GVOŽDÍK. Systematic position of the Clicking Frog (Kassinula Laurent, 1940), the problem of chimeric sequences and the revised classification of the family Hyperoliidae. Molecular Phylogenetics and Evolution. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2022, roč. 174, September, s. "107514", 11 s. ISSN 1055-7903. Dostupné z: https://doi.org/10.1016/j.ympev.2022.107514.

@article{1873879,
   author = {Nečas, Tadeáš and Kielgast, Jos and Nagy, Zoltán T. and Chifundera, Zacharie Kusamba and Gvoždík, Václav},
   article_number = {September},
   doi = {https://doi.org/10.1016/j.ympev.2022.107514},
   keywords = {Afrotropics; Amphibians; Congo Basin; Suprageneric classification; Systematics},
   language = {eng},
   issn = {1055-7903},
   journal = {Molecular Phylogenetics and Evolution},
   title = {Systematic position of the Clicking Frog (Kassinula Laurent, 1940), the problem of chimeric sequences and the revised classification of the family Hyperoliidae},
   url = {https://doi.org/10.1016/j.ympev.2022.107514},
   volume = {174},
   year = {2022}
}

TY  - JOUR
ID  - 1873879
AU  - Nečas, Tadeáš - Kielgast, Jos - Nagy, Zoltán T. - Chifundera, Zacharie Kusamba - Gvoždík, Václav
PY  - 2022
TI  - Systematic position of the Clicking Frog (Kassinula Laurent, 1940), the problem of chimeric sequences and the revised classification of the family Hyperoliidae
JF  - Molecular Phylogenetics and Evolution
VL  - 174
IS  - September
SP  - "107514"
EP  - "107514"
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 10557903
KW  - Afrotropics
KW  - Amphibians
KW  - Congo Basin
KW  - Suprageneric classification
KW  - Systematics
UR  - https://doi.org/10.1016/j.ympev.2022.107514
N2  - The systematics of the African frog family Hyperoliidae has undergone turbulent changes in last decades. Representatives of several genera have not been genetically investigated or with only limited data, and their phylogenetic positions are thus still not reliably known. This is the case of the De Witte's Clicking Frog (Kassinula wittei) which belongs to a monotypic genus. This miniature frog occurs in a poorly studied region, southeastern Democratic Republic of the Congo, northern Zambia, Angola. So far it is not settled whether this genus belongs to the subfamily Kassininae as a relative of the genus Kassina, or to the subfamily Hyperoliinae as a relative of the genus Afrixalus. Here we present for the first time a multilocus phylogenetic reconstruction (using five nuclear and one mitochondrial marker) of the family Hyperoliidae, including Kassinula. We demonstrate with high confidence that Kassinula is a member of Hyperoliinae belonging to a clade also containing Afrixalus (subSaharan Africa), Heterixalus (Madagascar) and Tachycnemis (Seychelles). We find that Kassinula represents a divergent lineage (17-25 Mya), which supports its separate genus-level status, but its exact systematic position remains uncertain. We propose to name the clade to which the above four genera belong as the tribe Tachycnemini Channing, 1989. A new taxonomy of the family Hyperoliidae was recently proposed by Dubois et al. (2021: Megataxa 5, 1-738). We demonstrate here that the new taxonomy was based on a partially erroneous phylogenetic reconstruction resulting from a supermatrix analysis of chimeric DNA sequences combining data from two families, Hyperoliidae and Arthroleptidae (the case of Cryptothylax). We therefore correct the erroneous part and propose a new, revised suprageneric taxonomy of the family Hyperoliidae. We also emphasize the importance of inspecting individual genetic markers before their concatenation or coalescent-based tree reconstructions to avoid analyses of chimeric DNA sequences producing incorrect phylogenetic reconstructions. Especially when phylogenetic reconstructions are used to propose taxonomies and systematic classifications.
ER  -

NEČAS, Tadeáš; Jos KIELGAST; Zoltán T. NAGY; Zacharie Kusamba CHIFUNDERA a Václav GVOŽDÍK. Systematic position of the Clicking Frog (Kassinula Laurent, 1940), the problem of chimeric sequences and the revised classification of the family Hyperoliidae. \textit{Molecular Phylogenetics and Evolution}. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2022, roč.~174, September, s.~''107514'', 11 s. ISSN~1055-7903. Dostupné z: https://doi.org/10.1016/j.ympev.2022.107514.

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