Use of sample preconcentration in CE-FA analyses

a 2022

Use of sample preconcentration in CE-FA analyses

BRŽEZICKÁ, Taťána, Lenka MICHALCOVÁ a Zdeněk GLATZ

Základní údaje

Originální název

Use of sample preconcentration in CE-FA analyses

Autoři

BRŽEZICKÁ, Taťána, Lenka MICHALCOVÁ a Zdeněk GLATZ

Vydání

Advances in chromatography and electrophoresis, Chiranal 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Prekoncentrace, CE-FA, afinitní interakce, BSA

Klíčová slova anglicky

Preconcentration, CE-FA, affinity interaction, BSA

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 6. 2024 11:55, Mgr. Taťána Bržezická

Anotace

V originále

Capillary electrophoresis (CE) technique is attractive for its rapid analysis, high efficiency, possible automation and nearness to physiological conditions. Despite of mentioned advantages of CE there is certain limitation of the technique, i.e. the low concentration sensitivity associated with the conventional ultraviolet visible (UV-VIS) detection. There are possibilities of increase the concentration sensititvity. Besides of exchanging the detection device with a more sensitive one, there are more elegant methods such as preconcentration techniques that allow the use of universal and less expensive UV-VIS detection. On-line preconcentration techniques allow automated analysis during separation while analytes are concentrated in the capillary [1]. As part of the miniaturization process for pharmacokinteic studies of plasma proteins-drug interactions, new more sensitive methods based on combination of the CE method with on line preconcentration techniques were optimized in this study. The established CE mode for affinity interaction studies is capillary electrophoresis-frontal analysis (CE-FA). The aim of this study was to optimize the new more sensitive CE-FA method in combination with on line preconcentration technique and the classical CE-FA method with similar conditions for data comparison. Another task was to consider evaluation of the binding properties of the drug plasma protein interaction by newly optimized method.

Návaznosti

GA19-08358S, projekt VaV

Název: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Grantová agentura ČR, Nove přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Citovat

BRŽEZICKÁ, Taťána, Lenka MICHALCOVÁ a Zdeněk GLATZ. Use of sample preconcentration in CE-FA analyses. In Advances in chromatography and electrophoresis, Chiranal 2022. 2022.

@proceedings{1875188,
   author = {Bržezická, Taťána and Michalcová, Lenka and Glatz, Zdeněk},
   booktitle = {Advances in chromatography and electrophoresis, Chiranal 2022},
   keywords = {Preconcentration, CE-FA, affinity interaction, BSA},
   language = {eng},
   title = {Use of sample preconcentration in CE-FA analyses},
   url = {https://gcms.cz/events/137},
   year = {2022}
}

TY  - CONF
ID  - 1875188
AU  - Bržezická, Taťána - Michalcová, Lenka - Glatz, Zdeněk
PY  - 2022
TI  - Use of sample preconcentration in CE-FA analyses
KW  - Preconcentration, CE-FA, affinity interaction, BSA
UR  - https://gcms.cz/events/137
N2  - Capillary electrophoresis (CE) technique is attractive for its rapid analysis, high efficiency, possible automation and nearness to physiological conditions. Despite of mentioned advantages of CE there is certain limitation of the technique, i.e. the low concentration sensitivity associated with the conventional ultraviolet visible (UV-VIS) detection. There are possibilities of increase the concentration sensititvity. Besides of exchanging the detection device with a more sensitive one, there are more elegant methods such as preconcentration techniques that allow the use of universal and less expensive UV-VIS detection. On-line preconcentration techniques allow automated analysis during separation while analytes are concentrated in the capillary [1]. As part of the miniaturization process for pharmacokinteic studies of plasma proteins-drug interactions, new more sensitive methods based on combination of the CE method with on line preconcentration techniques were optimized in this study. The established CE mode for affinity interaction studies is capillary electrophoresis-frontal analysis (CE-FA). The aim of this study was to optimize the new more sensitive CE-FA method in combination with on line preconcentration technique and the classical CE-FA method with similar conditions for data comparison. Another task was to consider evaluation of the binding properties of the drug plasma protein interaction by newly optimized method.
ER  -

BRŽEZICKÁ, Taťána, Lenka MICHALCOVÁ a Zdeněk GLATZ. Use of sample preconcentration in CE-FA analyses. In \textit{Advances in chromatography and electrophoresis, Chiranal 2022}. 2022.

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