The Effect of Hormonal Treatment on Selected Sperm Quality
Parameters and Sex Steroids in Tropical Cyprinid Bala Shark
Balantiocheilos melanopterus

J 2022

The Effect of Hormonal Treatment on Selected Sperm Quality Parameters and Sex Steroids in Tropical Cyprinid Bala Shark Balantiocheilos melanopterus

PODHOREC, P.; J. KNOWLES; Jakub VYSLOUŽIL; S. BORYSHPOLETS; A. SOTNIKOV et al.

Základní údaje

Originální název

The Effect of Hormonal Treatment on Selected Sperm Quality Parameters and Sex Steroids in Tropical Cyprinid Bala Shark Balantiocheilos melanopterus

Autoři

PODHOREC, P.; J. KNOWLES; Jakub VYSLOUŽIL ; S. BORYSHPOLETS; A. SOTNIKOV; Martina HOLICKÁ; J. KOURIL a B. DZYUBA

Vydání

Fishes, BASEL, MDPI, 2022, 2410-3888

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.300

Kód RIV

RIV/00216224:14160/22:00129441

Organizační jednotka

Farmaceutická fakulta

DOI

https://doi.org/10.3390/fishes7030122

UT WoS

000818344000001

EID Scopus

2-s2.0-85134414631

Klíčová slova anglicky

sperm; cyprinid; sustained release; reproduction; luteinizing hormone

Štítky

rivok, ÚFT

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 7. 2022 14:06, JUDr. Sabina Gajdzioková

Anotace

V originále

Simple Summary The bala shark Balantiocheilos melanopterus does not reproduce naturally under culture conditions, and hormone stimulation is routinely administered to male broodstock to ensure production of adequate quantities of high-quality sperm. Poly(lactic-co-glycolic acid) (PLGA) microparticles with slow release of 10 mu g/kg fish body weight GnRHa were injected to induce spermiation in bala sharks and to compare them with standard treatments in tropical cyprinid culture: recombinant hCG and GnRHa with metoclopramide. The PLGA microparticle treatment led to significantly higher plasma T and 11-KT concentrations at 24 h post-injection than was detected in the control group. Sperm motility parameters were evaluated during the 10-60 s after motility activation at 2 s intervals. Starting from 28 s post-activation, the motility percentage was significantly higher in the PLGA group compared to the control group. The PLGA microparticle treatment was also found to significantly increase sperm volume and total sperm count compared to control (0.9% NaCl). The PLGA microparticle system with continuous release of GnRHa was identified as optimal for inducing spermiation in cyprinid bala sharks. Hormone treatments are routinely employed with bala shark Balantiocheilos melanopterus male broodstock to stimulate the production of high-quality sperm. In the current trial, three spermiation-inducing treatments were evaluated: 20 mu g/kg body weight (BW) recombinant hCG; 20 mu g/kg BW [D-Ala(6), Pro(9), NEt]- gonadotropin-releasing hormone (GnRHa) with 20 mg/kg BW metoclopramide; and poly(lactic-co-glycolic acid) (PLGA) microparticles with slow release of 10 mu g/kg BW GnRHa. A 0.9% saline AS negative control was also included. Administration of the GnRHa through the form of slow release of PLGA microparticles 24 h after treatment resulted in a significantly higher sperm volume, motility percentage, and total sperm count compared to the control. Injection of GnRHa with metoclopramide induced sperm parameters that did not differ from the control, with the exception of motility percentage. The lowest potency to induce spermiation in bala sharks was in the treatment with recombinant hCG. Both PLGA microparticles and GnRHa with metoclopramide significantly increased blood plasma concentrations of testosterone and 11-ketotestosterone compared to the control. The PLGA microparticle system with continuous release of 10 mu g/kg BW GnRHa was the most effective treatment in inducing spermiation in bala sharks.

Návaznosti

QK1810221, projekt VaV

Název: Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Investor: Ministerstvo zemědělství ČR, Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Citovat

PODHOREC, P.; J. KNOWLES; Jakub VYSLOUŽIL; S. BORYSHPOLETS; A. SOTNIKOV; Martina HOLICKÁ; J. KOURIL a B. DZYUBA. The Effect of Hormonal Treatment on Selected Sperm Quality Parameters and Sex Steroids in Tropical Cyprinid Bala Shark Balantiocheilos melanopterus. Fishes. BASEL: MDPI, 2022, roč. 7, č. 3, s. 1-11. ISSN 2410-3888. Dostupné z: https://doi.org/10.3390/fishes7030122.

@article{1875879,
   author = {Podhorec, P. and Knowles, J. and Vysloužil, Jakub and Boryshpolets, S. and Sotnikov, A. and Holická, Martina and Kouril, J. and Dzyuba, B.},
   article_location = {BASEL},
   article_number = {3},
   doi = {https://doi.org/10.3390/fishes7030122},
   keywords = {sperm; cyprinid; sustained release; reproduction; luteinizing hormone},
   language = {eng},
   issn = {2410-3888},
   journal = {Fishes},
   title = {The Effect of Hormonal Treatment on Selected Sperm Quality Parameters and Sex Steroids in Tropical Cyprinid Bala Shark Balantiocheilos melanopterus},
   url = {https://www.mdpi.com/2410-3888/7/3/122},
   volume = {7},
   year = {2022}
}

TY  - JOUR
ID  - 1875879
AU  - Podhorec, P. - Knowles, J. - Vysloužil, Jakub - Boryshpolets, S. - Sotnikov, A. - Holická, Martina - Kouril, J. - Dzyuba, B.
PY  - 2022
TI  - The Effect of Hormonal Treatment on Selected Sperm Quality Parameters and Sex Steroids in Tropical Cyprinid Bala Shark Balantiocheilos melanopterus
JF  - Fishes
VL  - 7
IS  - 3
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 24103888
KW  - sperm
KW  - cyprinid
KW  - sustained release
KW  - reproduction
KW  - luteinizing hormone
UR  - https://www.mdpi.com/2410-3888/7/3/122
N2  - Simple Summary The bala shark Balantiocheilos melanopterus does not reproduce naturally under culture conditions, and hormone stimulation is routinely administered to male broodstock to ensure production of adequate quantities of high-quality sperm. Poly(lactic-co-glycolic acid) (PLGA) microparticles with slow release of 10 mu g/kg fish body weight GnRHa were injected to induce spermiation in bala sharks and to compare them with standard treatments in tropical cyprinid culture: recombinant hCG and GnRHa with metoclopramide. The PLGA microparticle treatment led to significantly higher plasma T and 11-KT concentrations at 24 h post-injection than was detected in the control group. Sperm motility parameters were evaluated during the 10-60 s after motility activation at 2 s intervals. Starting from 28 s post-activation, the motility percentage was significantly higher in the PLGA group compared to the control group. The PLGA microparticle treatment was also found to significantly increase sperm volume and total sperm count compared to control (0.9% NaCl). The PLGA microparticle system with continuous release of GnRHa was identified as optimal for inducing spermiation in cyprinid bala sharks. Hormone treatments are routinely employed with bala shark Balantiocheilos melanopterus male broodstock to stimulate the production of high-quality sperm. In the current trial, three spermiation-inducing treatments were evaluated: 20 mu g/kg body weight (BW) recombinant hCG; 20 mu g/kg BW [D-Ala(6), Pro(9), NEt]- gonadotropin-releasing hormone (GnRHa) with 20 mg/kg BW metoclopramide; and poly(lactic-co-glycolic acid) (PLGA) microparticles with slow release of 10 mu g/kg BW GnRHa. A 0.9% saline AS negative control was also included. Administration of the GnRHa through the form of slow release of PLGA microparticles 24 h after treatment resulted in a significantly higher sperm volume, motility percentage, and total sperm count compared to the control. Injection of GnRHa with metoclopramide induced sperm parameters that did not differ from the control, with the exception of motility percentage. The lowest potency to induce spermiation in bala sharks was in the treatment with recombinant hCG. Both PLGA microparticles and GnRHa with metoclopramide significantly increased blood plasma concentrations of testosterone and 11-ketotestosterone compared to the control. The PLGA microparticle system with continuous release of 10 mu g/kg BW GnRHa was the most effective treatment in inducing spermiation in bala sharks.
ER  -

PODHOREC, P.; J. KNOWLES; Jakub VYSLOUŽIL; S. BORYSHPOLETS; A. SOTNIKOV; Martina HOLICKÁ; J. KOURIL a B. DZYUBA. The Effect of Hormonal Treatment on Selected Sperm Quality Parameters and Sex Steroids in Tropical Cyprinid Bala Shark Balantiocheilos melanopterus. \textit{Fishes}. BASEL: MDPI, 2022, roč.~7, č.~3, s.~1-11. ISSN~2410-3888. Dostupné z: https://doi.org/10.3390/fishes7030122.

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