Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG
Response in Mice Immunized with Two Commercial Tick-Borne
Encephalitis Vaccines.

J 2022

Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines.

SALÁT, Jiří, Petra STRAKOVÁ and Daniel RŮŽEK

Basic information

Original name

Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines.

Authors

SALÁT, Jiří (203 Czech Republic), Petra STRAKOVÁ (203 Czech Republic) and Daniel RŮŽEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Vaccines, MDPI, 2022, 2076-393X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.800

RIV identification code

RIV/00216224:14310/22:00126325

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/vaccines10071001

UT WoS

000833195100001

Keywords in English

tick-borne encephalitis virus; vaccine; non-structural protein 1

Abstract

V originále

The presence of a non-structural protein 1 (NS1) in tick-borne encephalitis (TBE) vaccines and the possible induction of an NS1-specific immune response in vaccinated individuals remains a somewhat controversial topic. Previously, we detected the presence of NS1 in the Encepur TBE vaccine by mass spectrometry and found the induction of NS1-specific IgG antibodies in mice vaccinated with the FSME-Immun TBE vaccine. Here, in this follow-up study, we examined the dynamics and extent of the NS1-specific IgG response in mice vaccinated with these two vaccines in more detail and compared it with the IgG response to the whole virus (WV). Mice were vaccinated at two-week intervals with a total of six doses of each vaccine, and levels of IgG antibodies to TBE virus WV and NS1 were measured by ELISA after each dose. Both vaccines elicited a robust anti-WV IgG response after two doses. The Encepur vaccine did not elicit NS1-specific IgG even after all six doses. In contrast, the FSME-Immun vaccine triggered the production of NS1-specific IgG after four doses. The results indicate that FSME-Immun is the only vaccine that elicits an NS1-specific antibody response in mice. However, compared to WV-specific IgG, the NS1-specific response is weaker, and a higher number of doses is required to induce detectable levels of NS1-specific IgG antibodies.

Citovat

SALÁT, Jiří, Petra STRAKOVÁ and Daniel RŮŽEK. Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines. Vaccines. MDPI, 2022, vol. 10, No 7, p. 1-6. ISSN 2076-393X. Available from: https://dx.doi.org/10.3390/vaccines10071001.

@article{1876276,
   author = {Salát, Jiří and Straková, Petra and Růžek, Daniel},
   article_number = {7},
   doi = {http://dx.doi.org/10.3390/vaccines10071001},
   keywords = {tick-borne encephalitis virus; vaccine; non-structural protein 1},
   language = {eng},
   issn = {2076-393X},
   journal = {Vaccines},
   title = {Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines.},
   url = {https://www.mdpi.com/2076-393X/10/7/1001},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1876276
AU  - Salát, Jiří - Straková, Petra - Růžek, Daniel
PY  - 2022
TI  - Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines.
JF  - Vaccines
VL  - 10
IS  - 7
SP  - 1-6
EP  - 1-6
PB  - MDPI
SN  - 2076393X
KW  - tick-borne encephalitis virus
KW  - vaccine
KW  - non-structural protein 1
UR  - https://www.mdpi.com/2076-393X/10/7/1001
N2  - The presence of a non-structural protein 1 (NS1) in tick-borne encephalitis (TBE) vaccines and the possible induction of an NS1-specific immune response in vaccinated individuals remains a somewhat controversial topic. Previously, we detected the presence of NS1 in the Encepur TBE vaccine by mass spectrometry and found the induction of NS1-specific IgG antibodies in mice vaccinated with the FSME-Immun TBE vaccine. Here, in this follow-up study, we examined the dynamics and extent of the NS1-specific IgG response in mice vaccinated with these two vaccines in more detail and compared it with the IgG response to the whole virus (WV). Mice were vaccinated at two-week intervals with a total of six doses of each vaccine, and levels of IgG antibodies to TBE virus WV and NS1 were measured by ELISA after each dose. Both vaccines elicited a robust anti-WV IgG response after two doses. The Encepur vaccine did not elicit NS1-specific IgG even after all six doses. In contrast, the FSME-Immun vaccine triggered the production of NS1-specific IgG after four doses. The results indicate that FSME-Immun is the only vaccine that elicits an NS1-specific antibody response in mice. However, compared to WV-specific IgG, the NS1-specific response is weaker, and a higher number of doses is required to induce detectable levels of NS1-specific IgG antibodies.
ER  -

SALÁT, Jiří, Petra STRAKOVÁ and Daniel RŮŽEK. Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines. \textit{Vaccines}. MDPI, 2022, vol.~10, No~7, p.~1-6. ISSN~2076-393X. Available from: https://dx.doi.org/10.3390/vaccines10071001.

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