Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

J 2022

Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

FUKUSHIMA, Hiroshi; Siddharth S. MATIKONDA; Syed Muhammad USAMA; Aki FURUSAWA; Takuya KATO et al.

Základní údaje

Originální název

Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

Autoři

FUKUSHIMA, Hiroshi; Siddharth S. MATIKONDA; Syed Muhammad USAMA; Aki FURUSAWA; Takuya KATO; Lenka ŠTACKOVÁ; Petr KLÁN; Hisataka KOBAYASHI a Martin J. SCHNERMANN

Vydání

Journal of the American Chemical Society, Washington, American Chemical Society, 2022, 0002-7863

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 15.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00126376

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP

Štítky

14313010, 143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 6. 2025 09:24, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Citovat

FUKUSHIMA, Hiroshi; Siddharth S. MATIKONDA; Syed Muhammad USAMA; Aki FURUSAWA; Takuya KATO; Lenka ŠTACKOVÁ; Petr KLÁN; Hisataka KOBAYASHI a Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. Journal of the American Chemical Society. Washington: American Chemical Society, 2022, roč. 144, č. 25, s. 11075-11080. ISSN 0002-7863. Dostupné z: https://doi.org/10.1021/jacs.2c02962.

@article{1883610,
   author = {Fukushima, Hiroshi and Matikonda, Siddharth S. and Usama, Syed Muhammad and Furusawa, Aki and Kato, Takuya and Štacková, Lenka and Klán, Petr and Kobayashi, Hisataka and Schnermann, Martin J.},
   article_location = {Washington},
   article_number = {25},
   doi = {https://doi.org/10.1021/jacs.2c02962},
   keywords = {LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP},
   language = {eng},
   issn = {0002-7863},
   journal = {Journal of the American Chemical Society},
   title = {Cyanine Phototruncation Enables Spatiotemporal Cell Labeling},
   url = {https://pubs.acs.org/doi/10.1021/jacs.2c02962},
   volume = {144},
   year = {2022}
}

TY  - JOUR
ID  - 1883610
AU  - Fukushima, Hiroshi - Matikonda, Siddharth S. - Usama, Syed Muhammad - Furusawa, Aki - Kato, Takuya - Štacková, Lenka - Klán, Petr - Kobayashi, Hisataka - Schnermann, Martin J.
PY  - 2022
TI  - Cyanine Phototruncation Enables Spatiotemporal Cell Labeling
JF  - Journal of the American Chemical Society
VL  - 144
IS  - 25
SP  - 11075-11080
EP  - 11075-11080
PB  - American Chemical Society
SN  - 00027863
KW  - LYMPH-NODE
KW  - IN-VIVO
KW  - IDENTIFICATION
KW  - TRACKING
KW  - EGFP
UR  - https://pubs.acs.org/doi/10.1021/jacs.2c02962
N2  - Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control.
ER  -

FUKUSHIMA, Hiroshi; Siddharth S. MATIKONDA; Syed Muhammad USAMA; Aki FURUSAWA; Takuya KATO; Lenka ŠTACKOVÁ; Petr KLÁN; Hisataka KOBAYASHI a Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2022, roč.~144, č.~25, s.~11075-11080. ISSN~0002-7863. Dostupné z: https://doi.org/10.1021/jacs.2c02962.

Přehled o publikaci