FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI and Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. Journal of the American Chemical Society. Washington: American Chemical Society, 2022, vol. 144, No 25, p. 11075-11080. ISSN 0002-7863. Available from: https://dx.doi.org/10.1021/jacs.2c02962. |
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@article{1883610, author = {Fukushima, Hiroshi and Matikonda, Siddharth S. and Usama, Syed Muhammad and Furusawa, Aki and Kato, Takuya and Štacková, Lenka and Klán, Petr and Kobayashi, Hisataka and Schnermann, Martin J.}, article_location = {Washington}, article_number = {25}, doi = {http://dx.doi.org/10.1021/jacs.2c02962}, keywords = {LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP}, language = {eng}, issn = {0002-7863}, journal = {Journal of the American Chemical Society}, title = {Cyanine Phototruncation Enables Spatiotemporal Cell Labeling}, url = {https://pubs.acs.org/doi/10.1021/jacs.2c02962}, volume = {144}, year = {2022} }
TY - JOUR ID - 1883610 AU - Fukushima, Hiroshi - Matikonda, Siddharth S. - Usama, Syed Muhammad - Furusawa, Aki - Kato, Takuya - Štacková, Lenka - Klán, Petr - Kobayashi, Hisataka - Schnermann, Martin J. PY - 2022 TI - Cyanine Phototruncation Enables Spatiotemporal Cell Labeling JF - Journal of the American Chemical Society VL - 144 IS - 25 SP - 11075-11080 EP - 11075-11080 PB - American Chemical Society SN - 00027863 KW - LYMPH-NODE KW - IN-VIVO KW - IDENTIFICATION KW - TRACKING KW - EGFP UR - https://pubs.acs.org/doi/10.1021/jacs.2c02962 N2 - Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control. ER -
FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI and Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2022, vol.~144, No~25, p.~11075-11080. ISSN~0002-7863. Available from: https://dx.doi.org/10.1021/jacs.2c02962.
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