An immunocytochemical analysis of growing axons in a silicone
chamber prefilled with artificial sponge matrix

J 1996

An immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix

DUBOVÝ, Petr a Jana BEDNÁŘOVÁ

Základní údaje

Originální název

An immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix

Název česky

Imunohistochemická analýza rostoucích axonů v silikonové cisterně

Autoři

DUBOVÝ, Petr a Jana BEDNÁŘOVÁ

Vydání

Acta Histochemica, Germany, Fischer Verlag, 1996, 0065-1281

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/96:00024506

Organizační jednotka

Lékařská fakulta

UT WoS

A1996UK55300002

Klíčová slova anglicky

GAP-43; neurofilaments; peripheral nerve; silicone chamber; substance P; transection

Štítky

GAP-43, neurofilaments, Peripheral nerve, silicone chamber, substance P, transection

Změněno: 19. 6. 2009 13:57, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

We have used antibodies against growth associated protein (GAP-43), phosphorylated neurofilament protein of 200 kDa molecular weight (RT-97) and substance P (SP) to analyze regrowing axons and their features in a silicone chamber filled with resorbable sponge matrix within the first two weeks after sciatic nerve transection in the rat. Growing axons identified with the GAP-43 antibody extended over a distance of about 7 mm from the proximal stump at 7 days and grew over a 10 mm gap within 14 days. This is a markedly longer distance than in the case of the standard chamber model without artificial sponge matrix. The regrowing axons were labelled with RT-97 already on the 7th day up to a distance of 5 mm and they made up about 75% of all axons in the first segments. The number of RT-97-positive axons did not increase significantly over the next 7 days, although they could be identified over a longer distance. Some of the growing axons expressed SP-like immunoreactivity (LI) 14 days, but not 7 days after chamber application and constituted about 30% of all growing axons in the first segment. The SP-LI fibres also appeared to grow from the distal stump since they were found in larger numbers in the distal segments than in central ones. Those fibres accompanying blood vessels are probably sympathetic. Our findings demonstrate that axons are able to bridge a 10 mm gap within 14 days under appropriate substrate conditions, which are provided by the resorbable fibrin sponge.

Česky

Rychlost reinervace tubulizovaného nervu byla studována imunohistochemickou detekcí GAP43 a RT97.

Návaznosti

GA309/93/0134, projekt VaV

Název: Migrace a diferenciace Schwannových buněk v dospělém organizmu.

Investor: Grantová agentura ČR, Migrace a diferenciace Schwannových buněk v dospělém organizmu

Citovat

DUBOVÝ, Petr a Jana BEDNÁŘOVÁ. An immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix. Acta Histochemica. Germany: Fischer Verlag, 1996, roč. 98, č. 2, s. 123-130. ISSN 0065-1281.

@article{193373,
   author = {Dubový, Petr and Bednářová, Jana},
   article_location = {Germany},
   article_number = {2},
   keywords = {GAP-43; neurofilaments; peripheral nerve; silicone chamber; substance P; transection},
   language = {eng},
   issn = {0065-1281},
   journal = {Acta Histochemica},
   title = {An immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix},
   volume = {98},
   year = {1996}
}

TY  - JOUR
ID  - 193373
AU  - Dubový, Petr - Bednářová, Jana
PY  - 1996
TI  - An immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix
JF  - Acta Histochemica
VL  - 98
IS  - 2
SP  - 123
EP  - 123
PB  - Fischer Verlag
SN  - 00651281
KW  - GAP-43
KW  - neurofilaments
KW  - peripheral nerve
KW  - silicone chamber
KW  - substance P
KW  - transection
N2  - We have used antibodies against growth associated protein (GAP-43), phosphorylated neurofilament protein of 200 kDa molecular weight (RT-97) and substance P (SP) to analyze regrowing axons and their features in a silicone chamber filled with resorbable sponge matrix within the first two weeks after sciatic nerve transection in the rat. Growing axons identified with the GAP-43 antibody extended over a distance of about 7 mm from the proximal stump at 7 days and grew over a 10 mm gap within 14 days. This is a markedly longer distance than in the case of the standard chamber model without artificial sponge matrix. The regrowing axons were labelled with RT-97 already on the 7th day up to a distance of 5 mm and they made up about 75% of all axons in the first segments. The number of RT-97-positive axons did not increase significantly over the next 7 days, although they could be identified over a longer distance. Some of the growing axons expressed SP-like immunoreactivity (LI) 14 days, but not 7 days after chamber application and constituted about 30% of all growing axons in the first segment. The SP-LI fibres also appeared to grow from the distal stump since they were found in larger numbers in the distal segments than in central ones. Those fibres accompanying blood vessels are probably sympathetic. Our findings demonstrate that axons are able to bridge a 10 mm gap within 14 days under appropriate substrate conditions, which are provided by the resorbable fibrin sponge.
ER  -

DUBOVÝ, Petr a Jana BEDNÁŘOVÁ. An immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix. \textit{Acta Histochemica}. Germany: Fischer Verlag, 1996, roč.~98, č.~2, s.~123-130. ISSN~0065-1281.

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