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@article{199155, author = {Pantůček, Roman and Rosypalová, Alena and Doškař, Jiří and Kailerová, Jana and Růžičková, Vladislava and Borecká, Pavla and Snopková, Šárka and Horváth, Radek and Götz, Friedrich and Rosypal, Stanislav}, article_location = {USA}, article_number = {2}, language = {eng}, issn = {0042-6822}, journal = {Virology}, title = {The polyvalent staphylococcal phage 812: its host-range mutants and related phages}, url = {http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6WXR-45HR2X6-5M&_origin=EMFR&_version=1&md5=7e8df1f81817beede549ebfb91fc2f16}, volume = {246}, year = {1998} }
TY - JOUR ID - 199155 AU - Pantůček, Roman - Rosypalová, Alena - Doškař, Jiří - Kailerová, Jana - Růžičková, Vladislava - Borecká, Pavla - Snopková, Šárka - Horváth, Radek - Götz, Friedrich - Rosypal, Stanislav PY - 1998 TI - The polyvalent staphylococcal phage 812: its host-range mutants and related phages JF - Virology VL - 246 IS - 2 SP - 241 EP - 241 PB - Academic Press SN - 00426822 UR - http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6WXR-45HR2X6-5M&_origin=EMFR&_version=1&md5=7e8df1f81817beede549ebfb91fc2f16 N2 - Ninety-five percent of 782 culture collection strains, as well as hospital strains of Staphylococcus aureus subsp. aureus of different provenance and 43% of 89 culture collection strains of different coagulase-negative species of the genus Staphylococcus,were found to be sensitive to the polyvalent phage 812 or to at least one of its host-range mutants or to the polyvalent phages SK311, 131,and U16.Thus sensitivity to the polyvalent staphylococcal phages seems to be one of the common features of S. aureus subsp. aureus strains. The adsorption kinetics and one-step growth characteristics of the phages 812 and SK311 were estimated. Restriction genomic maps of the phages 812 (146.5 kb) and SK311 (141.1 kb) were constructed by use of the restriction endonucleases Ava II, Pst I, Kpn I, Sac I, Sma I, and Xho I. The host-range mutations of the phage 812 were localized on this map. Comparison of restriction patterns of the phages 812 and SK311 with those of the polyvalent phages U16 and 131 suggests that all these phages are closely related. Their genomes differ from each other mostly by some deletions, insertions (1-3 kb), or inversions. Evidence was given that the phage 812 together with SK311,131,and U16 belongs in the phage species Twort, the description of which is substantially supplemented with the data on the phage 812 reported in this paper. Copyright 1998 Academic Press. ER -
PANTŮČEK, Roman, Alena ROSYPALOVÁ, Jiří DOŠKAŘ, Jana KAILEROVÁ, Vladislava RŮŽIČKOVÁ, Pavla BORECKÁ, Šárka SNOPKOVÁ, Radek HORVÁTH, Friedrich GÖTZ a Stanislav ROSYPAL. The polyvalent staphylococcal phage 812: its host-range mutants and related phages. \textit{Virology}. USA: Academic Press, 1998, roč.~246, č.~2, s.~241-252. ISSN~0042-6822.
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