Confocal imaging of protein kinase C isozymes and pHi changes
in cardiac cells.

D 1998

Confocal imaging of protein kinase C isozymes and pHi changes in cardiac cells.

NOVÁKOVÁ, Marie; Michael YEDOVITZKY; R. NESHER a Yael EILAM

Základní údaje

Originální název

Confocal imaging of protein kinase C isozymes and pHi changes in cardiac cells.

Název česky

Zobrazení izoenzymů proteinkinázy C a změn intracelulárního pH u srdečních buněk pomocí konfokálního mikroskopu

Autoři

NOVÁKOVÁ, Marie; Michael YEDOVITZKY; R. NESHER a Yael EILAM

Vydání

14. vyd. Brno, Analysis of Biomedical Signals and Images, Biosignal 98, s. 249-251, 1998

Nakladatel

Vutium Press, Technical University Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

ISBN

80-214-1169-4

Klíčová slova anglicky

proteinkinase C;intracellular pH;confocal microscopy

Štítky

confocal microscopy, intracellular pH, proteinkinase C

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 6. 2009 20:04, prof. MUDr. Marie Nováková, Ph.D.

Anotace

ORIG CZ

V originále

In our experiments on isolated rat cardiac cells we studied the intracellular activation and translocation of protein kinase C (PKC) isozymes and pH changes using confocal microscopy. We detected the delta, kappa and mu isozymes of PKC in cardiac cells stimulated with phorbol ester, alpha-adrenergic agonist phenylephrine and different sigma receptor ligands. The histochemical imaging was performed on a PhioBos 1000 confocal microscope (Sarastro Inc., Ypsilanti, MI) equipped with Zeiss Universal Optics argon laser illumination. The changes of intracellular pH (pHi) following the incubation of the cells with the sigma receptor ligands were studied in the cells preincubated with pH-sensitive fluorescent indicator BCECF and then examined by a confocal scanning fluorescence microscope (ACAS 470 Interactive Laser Cytometer, Meridian). Confocal microscopy was proved superior and irreplaceable in studying the dynamics of intracellular signal transduction.

Česky

Práce se zabývá využitím konfokální mikroskopie pro detekci intracelulárních změn pH a exprese různých izoforem proteinkinázy C pod vlivem ligandů sigma receptorů u izolovaných kardiomyocytů potkana.

Citovat

NOVÁKOVÁ, Marie; Michael YEDOVITZKY; R. NESHER a Yael EILAM. Confocal imaging of protein kinase C isozymes and pHi changes in cardiac cells. In Analysis of Biomedical Signals and Images, Biosignal 98. 14. vyd. Brno: Vutium Press, Technical University Brno, 1998, s. 249-251. ISBN 80-214-1169-4.

@inproceedings{201194,
   author = {Nováková, Marie and Yedovitzky, Michael and Nesher, R. and Eilam, Yael},
   address = {Brno},
   booktitle = {Analysis of Biomedical Signals and Images, Biosignal 98},
   edition = {14},
   keywords = {proteinkinase C;intracellular pH;confocal microscopy},
   language = {eng},
   location = {Brno},
   isbn = {80-214-1169-4},
   pages = {249-251},
   publisher = {Vutium Press, Technical University Brno},
   title = {Confocal imaging of protein kinase C isozymes and pHi changes in cardiac cells.},
   year = {1998}
}

TY  - CONF
ID  - 201194
AU  - Nováková, Marie - Yedovitzky, Michael - Nesher, R. - Eilam, Yael
PY  - 1998
TI  - Confocal imaging of protein kinase C isozymes and pHi changes in cardiac cells.
PB  - Vutium Press, Technical University Brno
CY  - Brno
SN  - 8021411694
KW  - proteinkinase C;intracellular pH;confocal microscopy
N2  - In our experiments on isolated rat cardiac cells we studied the intracellular activation and translocation of protein kinase C (PKC) isozymes and pH changes using confocal microscopy. We detected the delta, kappa and mu isozymes of PKC in cardiac cells stimulated with phorbol ester, alpha-adrenergic agonist phenylephrine and different sigma receptor ligands. The histochemical imaging was performed on a PhioBos 1000 confocal microscope (Sarastro Inc., Ypsilanti, MI) equipped with Zeiss Universal Optics argon laser illumination. The changes of intracellular pH (pHi) following the incubation of the cells with the sigma receptor ligands were studied in the cells preincubated with pH-sensitive fluorescent indicator BCECF and then examined by a confocal scanning fluorescence microscope (ACAS 470 Interactive Laser Cytometer, Meridian). Confocal microscopy was proved superior and irreplaceable in studying the dynamics of intracellular signal transduction.
ER  -

NOVÁKOVÁ, Marie; Michael YEDOVITZKY; R. NESHER a Yael EILAM. Confocal imaging of protein kinase C isozymes and pHi changes in cardiac cells. In \textit{Analysis of Biomedical Signals and Images, Biosignal 98}. 14. vyd. Brno: Vutium Press, Technical University Brno, 1998, s.~249-251. ISBN~80-214-1169-4.

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