| KLEPÁRNÍK, Karel, J. BERKA, F. FORET, Jiří DOŠKAŘ, Jana KAILEROVÁ, Stanislav ROSYPAL a Petr BOČEK. DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide. Electrophoresis. Weinheim: VHC, 1998, roč. 19, č. 5, s. 695-700. ISSN 0173-0835. |
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@article{202187,
author = {Klepárník, Karel and Berka, J. and Foret, F. and Doškař, Jiří and Kailerová, Jana and Rosypal, Stanislav and Boček, Petr},
article_location = {Weinheim},
article_number = {5},
keywords = {Capillary electrophoresis; Staphylococcus aureus bacteriophage; DNA cycle sequencing; Linear polyacrylamide},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide},
url = {http://www3.interscience.wiley.com/cgi-bin/abstract/110465404/ABSTRACT},
volume = {19},
year = {1998}
}
TY - JOUR
ID - 202187
AU - Klepárník, Karel - Berka, J. - Foret, F. - Doškař, Jiří - Kailerová, Jana - Rosypal, Stanislav - Boček, Petr
PY - 1998
TI - DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide
JF - Electrophoresis
VL - 19
IS - 5
SP - 695
EP - 695
PB - VHC
SN - 01730835
KW - Capillary electrophoresis
KW - Staphylococcus aureus bacteriophage
KW - DNA cycle sequencing
KW - Linear polyacrylamide
UR - http://www3.interscience.wiley.com/cgi-bin/abstract/110465404/ABSTRACT
N2 - The nucleotide sequence of a part of a 4.9 kbp common restriction fragment isolated from Staphylococcus aureus bacteriophage (bacterial virus) 3A has been determined by capillary electrophoresis (CE). The fast separation of sequencing fragments in linear polyacrylamide solution at a temperature of 55C allowed the reading of more than 650 bases of sequence in 60 min. The single strand (ss)DNA fragments were prepared by cycle sequencing with fluo-rescently labeled dideoxy-terminators on the cloned bacteriophage DNA template. With respect to analysis speed, sequence read-length, low sample consumption and automation, CE offers a simple, labor-saving and inexpensive procedure for DNA sequencing. Operating the CE columns at elevated temperature proved to be a rapid procedure capable of extending sequence read-length. The resulting sequence of the common restriction fragment can be used for the preparation of specific primers and oligonucleotide hybridization probes for identification of Staphylococcus aureus bacteriophages and/or prophages belonging to the bacteriophage species 3A
ER -
KLEPÁRNÍK, Karel, J. BERKA, F. FORET, Jiří DOŠKAŘ, Jana KAILEROVÁ, Stanislav ROSYPAL a Petr BOČEK. DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide. \textit{Electrophoresis}. Weinheim: VHC, 1998, roč.~19, č.~5, s.~695-700. ISSN~0173-0835.
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