DNA cycle sequencing of a common restriction fragment of
Staphylococcus aureus bacteriophages by capillary
electrophoresis using replaceable linear polyacrylamide

J 1998

DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide

KLEPÁRNÍK, Karel; J. BERKA; F. FORET; Jiří DOŠKAŘ; Jana KAILEROVÁ et al.

Základní údaje

Originální název

DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide

Autoři

KLEPÁRNÍK, Karel; J. BERKA; F. FORET; Jiří DOŠKAŘ; Jana KAILEROVÁ; Stanislav ROSYPAL a Petr BOČEK

Vydání

Electrophoresis, Weinheim, VHC, 1998, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

[Abstract], [Full text]

Impakt faktor

Impact factor: 3.054

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/98:00000825

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000073692400015

Klíčová slova anglicky

Capillary electrophoresis; Staphylococcus aureus bacteriophage; DNA cycle sequencing; Linear polyacrylamide

Štítky

Capillary electrophoresis, DNA cycle sequencing, Linear polyacrylamide, Staphylococcus aureus bacteriophage

Změněno: 1. 12. 2005 21:35, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

The nucleotide sequence of a part of a 4.9 kbp common restriction fragment isolated from Staphylococcus aureus bacteriophage (bacterial virus) 3A has been determined by capillary electrophoresis (CE). The fast separation of sequencing fragments in linear polyacrylamide solution at a temperature of 55C allowed the reading of more than 650 bases of sequence in 60 min. The single strand (ss)DNA fragments were prepared by cycle sequencing with fluo-rescently labeled dideoxy-terminators on the cloned bacteriophage DNA template. With respect to analysis speed, sequence read-length, low sample consumption and automation, CE offers a simple, labor-saving and inexpensive procedure for DNA sequencing. Operating the CE columns at elevated temperature proved to be a rapid procedure capable of extending sequence read-length. The resulting sequence of the common restriction fragment can be used for the preparation of specific primers and oligonucleotide hybridization probes for identification of Staphylococcus aureus bacteriophages and/or prophages belonging to the bacteriophage species 3A

Návaznosti

GA301/97/1192, projekt VaV

Název: Molekulární identifikace a diagnostika stafylokoků způsobující nozokomiální infekce

Investor: Grantová agentura ČR, Molekulární identifikace a diagnostika stafylokoků způsobující nozokomiální infekce

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

KLEPÁRNÍK, Karel; J. BERKA; F. FORET; Jiří DOŠKAŘ; Jana KAILEROVÁ; Stanislav ROSYPAL a Petr BOČEK. DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide. Electrophoresis. Weinheim: VHC, 1998, roč. 19, č. 5, s. 695-700. ISSN 0173-0835.

@article{202187,
   author = {Klepárník, Karel and Berka, J. and Foret, F. and Doškař, Jiří and Kailerová, Jana and Rosypal, Stanislav and Boček, Petr},
   article_location = {Weinheim},
   article_number = {5},
   keywords = {Capillary electrophoresis; Staphylococcus aureus bacteriophage; DNA cycle sequencing; Linear polyacrylamide},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide},
   url = {http://www3.interscience.wiley.com/cgi-bin/abstract/110465404/ABSTRACT},
   volume = {19},
   year = {1998}
}

TY  - JOUR
ID  - 202187
AU  - Klepárník, Karel - Berka, J. - Foret, F. - Doškař, Jiří - Kailerová, Jana - Rosypal, Stanislav - Boček, Petr
PY  - 1998
TI  - DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide
JF  - Electrophoresis
VL  - 19
IS  - 5
SP  - 695
EP  - 695
PB  - VHC
SN  - 01730835
KW  - Capillary electrophoresis
KW  - Staphylococcus aureus bacteriophage
KW  - DNA cycle sequencing
KW  - Linear polyacrylamide
UR  - http://www3.interscience.wiley.com/cgi-bin/abstract/110465404/ABSTRACT
N2  - The nucleotide sequence of a part of a 4.9 kbp common restriction fragment isolated from Staphylococcus aureus bacteriophage (bacterial virus) 3A has been determined by capillary electrophoresis (CE). The fast separation of sequencing fragments in linear polyacrylamide solution at a temperature of 55C allowed the reading of more than 650 bases of sequence in 60 min. The single strand (ss)DNA fragments were prepared by cycle sequencing with fluo-rescently labeled dideoxy-terminators on the cloned bacteriophage DNA template. With respect to analysis speed, sequence read-length, low sample consumption and automation, CE offers a simple, labor-saving and inexpensive procedure for DNA sequencing. Operating the CE columns at elevated temperature proved to be a rapid procedure capable of extending sequence read-length. The resulting sequence of the common restriction fragment can be used for the preparation of specific primers and oligonucleotide hybridization probes for identification of Staphylococcus aureus bacteriophages and/or prophages belonging to the bacteriophage species 3A
ER  -

KLEPÁRNÍK, Karel; J. BERKA; F. FORET; Jiří DOŠKAŘ; Jana KAILEROVÁ; Stanislav ROSYPAL a Petr BOČEK. DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide. \textit{Electrophoresis}. Weinheim: VHC, 1998, roč.~19, č.~5, s.~695-700. ISSN~0173-0835.

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