Specific Determination of Glutathione using Micellar
Elektrokinetic Chromatography and On-Column Reaction with
2,2-dipyridyldisulphide

D 1999

Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide

GLATZ, Zdeněk a Hana MAŠLAŇOVÁ

Základní údaje

Originální název

Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide

Autoři

GLATZ, Zdeněk a Hana MAŠLAŇOVÁ

Vydání

Amsterdam, Netherlands, Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands, s. 10-10, 1999

Nakladatel

Elsevier, Netherlands

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/99:00003210

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Micellar Elektrokinetic Chromatography; On-Column Derivatization; Thiols; Glutathione

Štítky

Glutathione, Micellar Elektrokinetic Chromatography, On-Column Derivatization, Thiols

Změněno: 28. 2. 2001 09:58, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.

Návaznosti

GA525/97/0340, projekt VaV

Název: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů

Investor: Grantová agentura ČR, Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů

Citovat

GLATZ, Zdeněk a Hana MAŠLAŇOVÁ. Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide. In Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands. Amsterdam, Netherlands: Elsevier, Netherlands, 1999, s. 10.

@inproceedings{205215,
   author = {Glatz, Zdeněk and Mašlaňová, Hana},
   address = {Amsterdam, Netherlands},
   booktitle = {Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands},
   keywords = {Micellar Elektrokinetic Chromatography; On-Column Derivatization; Thiols; Glutathione},
   language = {eng},
   location = {Amsterdam, Netherlands},
   pages = {10-10},
   publisher = {Elsevier, Netherlands},
   title = {Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide},
   year = {1999}
}

TY  - CONF
ID  - 205215
AU  - Glatz, Zdeněk - Mašlaňová, Hana
PY  - 1999
TI  - Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide
PB  - Elsevier, Netherlands
CY  - Amsterdam, Netherlands
KW  - Micellar Elektrokinetic Chromatography
KW  - On-Column Derivatization
KW  - Thiols
KW  - Glutathione
N2  - The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
ER  -

GLATZ, Zdeněk a Hana MAŠLAŇOVÁ. Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide. In \textit{Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands}. Amsterdam, Netherlands: Elsevier, Netherlands, 1999, s.~10.

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