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@inproceedings{213991,
author = {Glatz, Zdeněk and Mašlaňová, Hana},
address = {Bratislava},
booktitle = {Proceeding of 13th International Conference},
keywords = {Glutathione; MEKC; 2;2-dipyridyldisulphide},
language = {eng},
location = {Bratislava},
isbn = {1335-5236},
pages = {25-26},
publisher = {Slovenská chemická společnosť pri SAV, Bratislava},
title = {Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography},
year = {1999}
}
TY - JOUR
ID - 213991
AU - Glatz, Zdeněk - Mašlaňová, Hana
PY - 1999
TI - Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography
PB - Slovenská chemická společnosť pri SAV, Bratislava
CY - Bratislava
SN - 13355236
KW - Glutathione
KW - MEKC
KW - 2;2-dipyridyldisulphide
N2 - The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
ER -
GLATZ, Zdeněk a Hana MAŠLAŇOVÁ. Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography. In \textit{Proceeding of 13th International Conference}. Bratislava: Slovenská chemická společnosť pri SAV, Bratislava, 1999, s.~25-26. ISBN~1335-5236.
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