On-Line MALDI-TOF MS Using Continuous Vacuum Deposition
Interface

J 1998

On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface

PREISLER, J., F. FORET a B. L. KARGER

Základní údaje

Originální název

On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface

Název česky

On-line MALDI-TOF MS pomocí rozhraní s kontinuálním vakuovým nanášením

Autoři

PREISLER, J. (203 Česká republika, garant), F. FORET (203 Česká republika) a B. L. KARGER (840 Spojené státy)

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 1998, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.580

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000077550100025

Klíčová slova anglicky

On-line; MALDI; TOFMS; Vacuum Deposition; Interface; Mass Spectrometry; Capillary Electrophoresis

Štítky

Capillary electrophoresis, interface, MALDI, mass spectrometry, On-line, TOFMS, vacuum deposition

Změněno: 28. 6. 2009 00:03, prof. Mgr. Jan Preisler, Ph.D.

Anotace

ORIG CZ

V originále

In this work, a new interface for continuous liquid on-line MALDI-TOF MS is presented. The sample, mixed with conventional matrix, is brought via a capillary inlet into the evacuated source chamber of the mass spectrometer at liquid flow rates of 200 to 400 nL/min. The liquid sample matrix is deposited on a rotating wheel, in the present study quartz, and transported to the repeller where laser desorption takes place. Rapid evaporation of the solvent (water or methanol) results in formation of a thin, ~ 50 um wide, sample trace. No crystals were observed in the trace using a scanning electron microscope, suggesting either very small sized crystals or an amorphous film. This sample uniformity resulted in excellent spot-to-spot reproducibility, with detection limits in the attomole range. Furthermore, and importantly, the interface permits the on-line coupling of high resolution microcolumn separation techniques with MALDI-MS, as demonstrated in the capillary electrophoresis - MALDI-TOF MS analysis of a 12 peptide mixture. The approach offers the potential of rapid, trace separation and analysis of complex mixtures.

Česky

On-line MALDI-TOF MS pomocí rozhraní s kontinuálním vakuovým nanášením, viz anglický popis.

Citovat

PREISLER, J., F. FORET a B. L. KARGER. On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 1998, roč. 70, č. 24, s. 5278-5287. ISSN 0003-2700.

@article{216631,
   author = {Preisler, J. and Foret, F. and Karger, B. L.},
   article_location = {Washington, D.C., USA},
   article_number = {24},
   keywords = {On-line; MALDI; TOFMS; Vacuum Deposition; Interface; Mass Spectrometry; Capillary Electrophoresis},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface},
   volume = {70},
   year = {1998}
}

TY  - JOUR
ID  - 216631
AU  - Preisler, J. - Foret, F. - Karger, B. L.
PY  - 1998
TI  - On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface
JF  - Analytical Chemistry
VL  - 70
IS  - 24
SP  - 5278
EP  - 5278
PB  - American Chemical Society
SN  - 00032700
KW  - On-line
KW  - MALDI
KW  - TOFMS
KW  - Vacuum Deposition
KW  - Interface
KW  - Mass Spectrometry
KW  - Capillary Electrophoresis
N2  - In this work, a new interface for continuous liquid on-line MALDI-TOF MS is presented. The sample, mixed with conventional matrix, is brought via a capillary inlet into the evacuated source chamber of the mass spectrometer at liquid flow rates of 200 to 400 nL/min. The liquid sample matrix is deposited on a rotating wheel, in the present study quartz, and transported to the repeller where laser desorption takes place. Rapid evaporation of the solvent (water or methanol) results in formation of a thin, ~ 50 um wide, sample trace. No crystals were observed in the trace using a scanning electron microscope, suggesting either very small sized crystals or an amorphous film. This sample uniformity resulted in excellent spot-to-spot reproducibility, with detection limits in the attomole range. Furthermore, and importantly, the interface permits the on-line coupling of high resolution microcolumn separation techniques with MALDI-MS, as demonstrated in the capillary electrophoresis - MALDI-TOF MS analysis of a 12 peptide mixture. The approach offers the potential of rapid, trace separation and analysis of complex mixtures.
ER  -

PREISLER, J., F. FORET a B. L. KARGER. On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 1998, roč.~70, č.~24, s.~5278-5287. ISSN~0003-2700.

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