Analysis of Artichoke Extract (Cynara cardunculus) by Micellar Electrokinetic Chromatography

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSIL. Analysis of Artichoke Extract (Cynara cardunculus) by Micellar Electrokinetic Chromatography. In Book of Abstract Chiranal. Olomouc: Česká společnost chemická, 1999, s. 57-58.

Základní údaje

Originální název

Analysis of Artichoke Extract (Cynara cardunculus) by Micellar Electrokinetic Chromatography

Autoři

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSIL

Vydání

Olomouc, Book of Abstract Chiranal, s. 57-58, 1999

Nakladatel

Česká společnost chemická

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Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

MEKC Artichoke

Štítky

MEKC Artichoke

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Anotace

V originále

Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative and hepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds present in the extract and to its caffeoylquinic derivatives. Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ion strength of the background electrolyte, SDS concentration and temperature. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic acid derivatives.