Endocrine-disrupting chemicals affect Sertoli TM4 cell
functionality through dysregulation of gap junctional
intercellular communication in vitro

J 2022

Endocrine-disrupting chemicals affect Sertoli TM4 cell functionality through dysregulation of gap junctional intercellular communication in vitro

YAWER, Affiefa; Eliška SYCHROVÁ; Jan RAŠKA; Pavel BABICA; Iva SOVADINOVÁ et al.

Základní údaje

Originální název

Endocrine-disrupting chemicals affect Sertoli TM4 cell functionality through dysregulation of gap junctional intercellular communication in vitro

Autoři

YAWER, Affiefa; Eliška SYCHROVÁ ; Jan RAŠKA; Pavel BABICA a Iva SOVADINOVÁ

Vydání

Food and Chemical Toxicology, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2022, 0278-6915

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00126426

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Connexins; Endocrine-disrupting chemicals; Gap junctional intercellular communication; Reproductive toxicity; Sertoli cells; Testicular tumors

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 8. 2025 15:23, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The frequencies of adverse outcomes associated with male reproductive health, including infertility and testicular cancer, are increasing. These adverse trends are partially attributed to increased exposure to environmental agents such as endocrine-disrupting chemicals (EDCs). This study addresses effects on EDCs on adjacent prepubertal Sertoli TM4 cells, specifically on 1) testicular gap junctional intercellular communication (GJIC), one of the hallmarks of non-genotoxic carcinogenicity, 2) GJIC building blocks connexins (Cx), and 3) mitogen-activated protein kinases MAPKs. We selected eight representatives of EDCs: organochlorine chemicals such as pesticides dichlorodiphenyltrichlomethane, lindane, methoxychlor, and vinclozolin, industrial chemicals bisphenol A and 2,2',4,4',5,5'-hexachlorobiphenyl, and components of personal care products, triclocarban and triclosan. EDCs rapidly dysregulated GJIC in Sertoli TM4 cells mainly via MAPK p38 and/or Erk1/2 pathways by the intermediate hyper- or de-phosphorylation of Cx43 (Ser368, Ser282) and translocation of Cx43 from the plasma membrane, suggesting disturbed intracellular trafficking of Cx43 protein. Surprisingly, EDCs did not rapidly activate MAPK Erk1/2 or p38; on the contrary, TCC and TCS decreased their activity (phosphorylation). Our results indicate that EDCs might disrupt testicular homeostasis and development via testicular GJIC, junctional and non-junctional functions of Cx43 and MAPK-signaling pathways in Sertoli cells.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GA22-30004S, projekt VaV

Název: Alternativní přístupy v hodnocení mužské reprodukční toxicity chemických směsí bez využití zvířat

Investor: Grantová agentura ČR, Alternativní přístupy v hodnocení mužské reprodukční toxicity chemických směsí bez využití zvířat

GJ16-10775Y, projekt VaV

Název: Mezibuněčná komunikace zprostředkovaná mezerovými spoji jako cíl endokrinních disruptorů v testikulárních buňkách

Investor: Grantová agentura ČR, Mezibuněčná komunikace zprostředkovaná mezerovými spoji jako cíl endokrinních disruptorů v testikulárních buňkách

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Citovat

YAWER, Affiefa; Eliška SYCHROVÁ; Jan RAŠKA; Pavel BABICA a Iva SOVADINOVÁ. Endocrine-disrupting chemicals affect Sertoli TM4 cell functionality through dysregulation of gap junctional intercellular communication in vitro. Food and Chemical Toxicology. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2022, roč. 164, June 2022, s. 1-13. ISSN 0278-6915. Dostupné z: https://doi.org/10.1016/j.fct.2022.113004.

@article{2211082,
   author = {Yawer, Affiefa and Sychrová, Eliška and Raška, Jan and Babica, Pavel and Sovadinová, Iva},
   article_location = {OXFORD},
   article_number = {June 2022},
   doi = {https://doi.org/10.1016/j.fct.2022.113004},
   keywords = {Connexins; Endocrine-disrupting chemicals; Gap junctional intercellular communication; Reproductive toxicity; Sertoli cells; Testicular tumors},
   language = {eng},
   issn = {0278-6915},
   journal = {Food and Chemical Toxicology},
   title = {Endocrine-disrupting chemicals affect Sertoli TM4 cell functionality through dysregulation of gap junctional intercellular communication in vitro},
   url = {https://www.sciencedirect.com/science/article/pii/S0278691522002022?via%3Dihub},
   volume = {164},
   year = {2022}
}

TY  - JOUR
ID  - 2211082
AU  - Yawer, Affiefa - Sychrová, Eliška - Raška, Jan - Babica, Pavel - Sovadinová, Iva
PY  - 2022
TI  - Endocrine-disrupting chemicals affect Sertoli TM4 cell functionality through dysregulation of gap junctional intercellular communication in vitro
JF  - Food and Chemical Toxicology
VL  - 164
IS  - June 2022
SP  - 1-13
EP  - 1-13
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 02786915
KW  - Connexins
KW  - Endocrine-disrupting chemicals
KW  - Gap junctional intercellular communication
KW  - Reproductive toxicity
KW  - Sertoli cells
KW  - Testicular tumors
UR  - https://www.sciencedirect.com/science/article/pii/S0278691522002022?via%3Dihub
N2  - The frequencies of adverse outcomes associated with male reproductive health, including infertility and testicular cancer, are increasing. These adverse trends are partially attributed to increased exposure to environmental agents such as endocrine-disrupting chemicals (EDCs). This study addresses effects on EDCs on adjacent prepubertal Sertoli TM4 cells, specifically on 1) testicular gap junctional intercellular communication (GJIC), one of the hallmarks of non-genotoxic carcinogenicity, 2) GJIC building blocks connexins (Cx), and 3) mitogen-activated protein kinases MAPKs. We selected eight representatives of EDCs: organochlorine chemicals such as pesticides dichlorodiphenyltrichlomethane, lindane, methoxychlor, and vinclozolin, industrial chemicals bisphenol A and 2,2',4,4',5,5'-hexachlorobiphenyl, and components of personal care products, triclocarban and triclosan. EDCs rapidly dysregulated GJIC in Sertoli TM4 cells mainly via MAPK p38 and/or Erk1/2 pathways by the intermediate hyper- or de-phosphorylation of Cx43 (Ser368, Ser282) and translocation of Cx43 from the plasma membrane, suggesting disturbed intracellular trafficking of Cx43 protein. Surprisingly, EDCs did not rapidly activate MAPK Erk1/2 or p38; on the contrary, TCC and TCS decreased their activity (phosphorylation). Our results indicate that EDCs might disrupt testicular homeostasis and development via testicular GJIC, junctional and non-junctional functions of Cx43 and MAPK-signaling pathways in Sertoli cells.
ER  -

YAWER, Affiefa; Eliška SYCHROVÁ; Jan RAŠKA; Pavel BABICA a Iva SOVADINOVÁ. Endocrine-disrupting chemicals affect Sertoli TM4 cell functionality through dysregulation of gap junctional intercellular communication in vitro. \textit{Food and Chemical Toxicology}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2022, roč.~164, June 2022, s.~1-13. ISSN~0278-6915. Dostupné z: https://doi.org/10.1016/j.fct.2022.113004.

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