MACHÁT, Radek, Lubomir POJEZDAL, Jan GEBAUER, Ján MATIAŠOVIC, Radek TESAŘÍK, Hana MINAROVA, Nikola HODKOVICOVA a Martin FALDYNA. Early immune response of two common carp breeds to koi herpesvirus infection. Fish and Shellfish Immunology. Elsevier, 2022, roč. 128, September, s. 206-215. ISSN 1050-4648. Dostupné z: https://dx.doi.org/10.1016/j.fsi.2022.07.064. |
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@article{2211630, author = {Machát, Radek and Pojezdal, Lubomir and Gebauer, Jan and Matiašovic, Ján and Tesařík, Radek and Minarova, Hana and Hodkovicova, Nikola and Faldyna, Martin}, article_number = {September}, doi = {http://dx.doi.org/10.1016/j.fsi.2022.07.064}, keywords = {qRT-PCR; Mass spectrometry; Koi carp; Amur wild carp; Complement cascade; Interferon class I; Cell-mediated cytotoxicity}, language = {eng}, issn = {1050-4648}, journal = {Fish and Shellfish Immunology}, title = {Early immune response of two common carp breeds to koi herpesvirus infection}, url = {https://doi.org/10.1016/j.fsi.2022.07.064}, volume = {128}, year = {2022} }
TY - JOUR ID - 2211630 AU - Machát, Radek - Pojezdal, Lubomir - Gebauer, Jan - Matiašovic, Ján - Tesařík, Radek - Minarova, Hana - Hodkovicova, Nikola - Faldyna, Martin PY - 2022 TI - Early immune response of two common carp breeds to koi herpesvirus infection JF - Fish and Shellfish Immunology VL - 128 IS - September SP - 206-215 EP - 206-215 PB - Elsevier SN - 10504648 KW - qRT-PCR KW - Mass spectrometry KW - Koi carp KW - Amur wild carp KW - Complement cascade KW - Interferon class I KW - Cell-mediated cytotoxicity UR - https://doi.org/10.1016/j.fsi.2022.07.064 N2 - Economic importance of common carp (Cyprinus carpio L.) increases every year. Viral diseases are major threat for carp aquaculture and cause significant economic losses. Koi herpesvirus (KHV) is one of the most serious carp diseases. Current study is focused on confirmation of possible differences in early immune response to KHV depending on level of resistance. Class I interferon signalling, complement cascade and cell-mediated cytotoxicity are hypothesized as major mechanisms of early innate immune response against KHV. Different breeds of common carp show distinct level of resistance to KHV. Two breeds of common carp with completely different susceptibility to KHV were chosen for current research: amur wild carp (AS) as highly resistant and koi carp (KOI) as very susceptible breed. KHV infection caused no mortalities, but the viral load in selected tissues increased during infection. Levels of expressions of chosen genes was examined using qRT-PCR and overall change in protein expression profiles was analysed by mass spectrometry. Significant differences in immune response between AS and KOI were detected mostly at the level of protein expression. Although cell-mediated cytotoxicity showed minimal influence during KHV infection, many immune response parameters related to class I interferon signalling pathway and complement cascade were increased earlier during KHV infection in AS comparing to KOI. ER -
MACHÁT, Radek, Lubomir POJEZDAL, Jan GEBAUER, Ján MATIAŠOVIC, Radek TESAŘÍK, Hana MINAROVA, Nikola HODKOVICOVA a Martin FALDYNA. Early immune response of two common carp breeds to koi herpesvirus infection. \textit{Fish and Shellfish Immunology}. Elsevier, 2022, roč.~128, September, s.~206-215. ISSN~1050-4648. Dostupné z: https://dx.doi.org/10.1016/j.fsi.2022.07.064.
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